Toward multidimensional information: A derivatization-free UHPLC-QqQ MS/MS method for amino acid components of fingerprint

The analysis of fingerprint chemical composition is a meaningful way to excavate the multidimensional information of fingerprint, including the donor profiling information and the age of a fingerprint, which broadens the evidential values of fingerprint, especially for the partial and distorted fingerprint. But the research remains still in the pilot phases or is ongoing. Amino acids are the dominant organic substances in latent sweat fingerprint and influenced by many donor factors. Hence, their content reflects personal information of donors. Forensic science will be revolutionized if suspects can be individualized by their amino acid content. The diverse nature, distinct physicochemical properties, and ultra-micro levels of amino acids present in fingerprints make it hard to detect. A high sensitivity method for detecting and quantifying multiple amino acid components is required. UHPLC-QqQ MS/MS offers high sensitivity, high separation, simultaneous multicomponents detection, and no derivatization, making it an ideal method for detecting and analyzing amino acids in fingerprints. Therefore, in this study, we propose and validate an efficient UHPLC-QqQ MS/MS method for the extraction and analysis of 13 amino acids from fingerprint. We compared the results of amino acids of 10 different substrates and found that the inherent amino acids in most porous substrates would have been extracted along with the fingerprint amino acids, making them unsuitable for quantitative amino acid analysis. Instead, plastic sheets are ideal substrates for laboratory studies. Then, extensive experiments were conducted among 30 donors for multidimensional information analysis. The type of samples analyzed were eccrine-rich fingerprints. A Binary Logistic Regression (BLR) model was developed, and the female and male donors were successfully differentiated by amino acids in fingerprints. Two other mathematical models were also developed to verify the accuracy, and all three different mathematical models were able to identify donors of different genders with over 90% accuracy. This demonstrates that amino acids have the potential to provide more information for donors as metabolic markers. In the future, we will conduct a series of experiments to analyze more multidimensional information for individual identification by amino acid content in the fingerprint.     

Wang Xiaoling and the Winglaiyuen Brand

正SINCE its establishment in 1947, the Winglaiyuen restaurant has become a well-known Hong Kong brand with an international reputation. Its development and expansion is largely due to one woman’s single-minded devotion.She is Wang Xiaoling."Running a successful restaurant is lucrative, but also hard work, because it demands whole-hearted devotion. Many brands build     

The Wonders of Dance

正SEVERAL months ago,Chen Wei and former university classmate Hu Yulin got married.Looking back on their lives together in university,Hu had a crush on her,but she had no feelings for him–yet.Born into an impoverished rural family,Hu Yulin was quite small when he became entranced by the melodies of a flute vender,so he bought a flute for two yuan.He recalls,"From then on,I taught myself,for there was no one to teach me."At the age of 17,Hu went to Beijing to be mentored.With Chen e oking their t feelings mpoverished rural famquite small when he d f o e n on,or there was e."7,Hu went to ntored.With     

Lu Xun at Home

正LU Xun(1881-1936)is widely considered as China’s greatest modern writer.Although he was a prolific essayist and translator,it is for his intense and often disturbing short stories that he is best known.In criticizing repressive social norms,he aimed for nothing less than the eradication of the nation’s spiritual ills.In the decades since his death editions of his works,translations and critical studies have surged,making Lu Xun an accessible writer in most major languages.But he can also be remembered through the homes in which he lived and worked.     

Oversight Effectiveness and Political Will: Some Lessons from West Africa

The purpose of the present note is to advance two theoretical claims. The first claim proposed is that the impact of the availability of oversight tools and of the most broadly understood legislative capacity (availability of material, technical, financial resources; availability of well-trained staff) on the effectiveness with which legislative oversight is performed is conditional. The second claim put forward, after reviewing a rich body of work on executive–legislative relations and legislative oversight in West Africa, is that, of the various conditions that promote or prevent the effective use of oversight tools and capacity, political will is the single most important. These claims have both theoretical and practical relevance, for if political will is as important as is claimed for the effective performance of the oversight function, then international organisations may have to reconsider their approach to legislative strengthening.     

Subaltern connections: Brazilian critical geographers,development and African decolonisation

Abstract

This article explores the relations to Africa and African decolonisation of three key figures in Brazilian critical geographies and development studies, Manuel Correia de Andrade (1922–2007), Josué de Castro (1908–1973) and Milton Santos (1926–2001). Based on an analysis of their works and unpublished archives, I argue the radical Third World perspectives these intellectuals expressed anticipated later critiques of development as a neocolonial device. Drawing upon current literature on decolonisation, international conferencing and anti-racist solidarity networks, I discuss these matters in relation to these authors’ interest in cultural diversity and internal colonialism. Crucially, they developed this sensitivity in the Brazilian Northeast, a region especially shaped by Afro–Brazilian and Indigenous cultural legacies. While supporting anti-imperialist nationalisms in the Third World, these Brazilian scholars fostered multilingual, internationalist and cosmopolitan activism and scholarship. This is revealed by the study of the transnational networks they developed during exile and the various persecutions that many of them suffered after the 1964 military coup. Finally, I argue these works can substantiate recent claims to ‘decolonise’ geography and development studies, on the condition that these fields of study take seriously their anti-imperial traditions and their ‘voices from the South’.     

State transformation goes nuclear: Chinese National Nuclear Companies’ expansion into Europe

Abstract

Most of the literature on state transformation focuses on China’s relations with African, Asian and Latin American countries and the National Oil Companies’ overseas expansion to show that China has become fragmented, decentralised and internationalised. This article contributes novel findings by focusing on China’s relations with Europe and the actions of China’s National Nuclear Companies (NNCs). It shows that NNCs, which have become relatively autonomous actors, often pursue their agendas of expansion into Europe without much coordination with, or even in contradiction to, other ministries’ agendas and interests, especially the Ministry of Foreign Affairs. Instead of being orchestrated by the central government, their expansion reflects considerable disorganisation and sometimes undermines China’s official strategy. The article demonstrates this through case studies of NNCs’ involvement in the UK and Romania.     

Chinese Eastern Industrial Zone in Ethiopia: unpacking the enclave

Abstract

This paper examines China’s engagement with Africa through economic zones (EZs). It moves beyond the conceptualisation of EZs as undifferentiated enclaves of foreign investment to a dynamic perspective on the locally negotiated process of zone development. Such a perspective entails critical unpacking of the specific zone regime to understand the diverse and evolving relationships among different state and non-state actors. Drawing upon empirical research on the Eastern Industrial Zone (EIZ) in Ethiopia, we explore the complex process of learning and adaptation by government, developers, investors, and workers throughout the development of a zone regime, with specific attention to capital–labour and expatriate–local relations. We find that despite the EIZ being a state-level cooperation project, private Chinese developers work diligently with the Ethiopian government to improve the institutional support for EZs. Chinese investors also collectively generate a management regime to enhance their overseas operational capacity and experiment with various tactics to transform local recruits into an industrial workforce. Local workers, with limited protection by official labour unions, turn to individual- and group-based agency to improve their working conditions. Despite the momentum created by multiple stakeholders, there are concerns regarding the long-term contributions of EZs to engender sustained industrial transformation and skills development.     

Transnational cooperation: a network analysis of town twinning in the Baltic Sea region

The patterns of cooperation between regions and urban spaces are no longer influenced, to the same extent, by national borders. Political integrative events changed the extent of transborder urban cooperation in the Baltic borderlands, a region that has undergone extensive changes. A social network analysis of cooperative ties in the form of town twinning indicates that the end of the Cold War strongly changed the network of town twinning in this region, whereas the effect of the various EU enlargement rounds seems to be more nuanced.     

Evaluation of canine training aids containment for homemade explosive and components by headspace analysis and canine testing

While canines are most commonly trained to detect traditional explosives, such as nitroaromatics and smokeless powders, homemade explosives (HMEs), such as fuel–oxidizer mixtures, are arguably a greater threat. As such, it is imperative that canines are sufficiently trained in the detection of such HMEs. The training aid delivery device (TADD) is a primary containment device that has been used to house HMEs and HME components for canine detection training purposes. This research assesses the odor release from HME components, ammonium nitrate (AN), urea nitrate (UN), and potassium chlorate (PC), housed in TADDs. Canine odor recognition tests (ORTs) were used with analytical data to determine the detectability of TADDs containing AN, UN, or PC. Headspace analysis by gas chromatography/mass spectrometry (GC/MS) with solid-phase microextraction (SPME) or online cryotrapping were used to measure ammonia or chlorine, as well as other unwanted odorants, emanating from bulk AN, UN, and PC in TADDs over 28 weeks. The analytical data showed variation in the amount of ammonia and chlorine over time, with ammonia from AN and UN decreasing slowly over time and the abundance of chlorine from PC TADDs dependent on the frequency of exposure to ambient air. Even with these variations in odor abundance, canines previously trained to detect bulk explosive HME components were able to detect all three targets in glass and plastic TADDs for at least 18 months after loading. Detection proficiency ranged from 64% to 100% and was not found to be dependent on either age of material.