灌服毒鼠强诱导大鼠细胞DNA的损伤研究

目的研究毒鼠强体内染毒后,毒鼠强对大鼠脑细胞、心肌细胞、淋巴细胞DNA的损伤作用。方法选择健康Sprague-Dawley大白鼠20只,分成5组,每组4只,采用灌胃方法使大鼠毒鼠强体内染毒,按0.2,0.1,0.05,0.01mg·kg-1制作大鼠毒鼠强中毒模型,并以灌服生理盐水的健康大鼠为对照,分离实验大鼠的淋巴细胞、心肌细胞和脑细胞,用彗星电泳的方法测定不同浓度毒鼠强中毒后的细胞DNA损伤。结果0.2,0.1,0.05,0.01mg·kg-1剂量组的毒鼠强均可引起大鼠的淋巴细胞、心肌细胞和脑细胞DNA损伤,均与对照组差异有显著性(P<0.01)。结论毒鼠强诱导体内细胞DNA损伤可能是毒鼠强毒性作用机制之一。     

FTIR图像—单点模式法无损鉴别不同品牌激光打印机墨粉色痕

利用傅里叶红外图像系统,采用单点模式对收集到的八种品牌激光打印机墨粉色痕进行红外扫描,采集样品红外光谱,并对其中两种样品红外光谱进行二阶导数运算,得到对应的二阶导数光谱。实验表明,该方法可有效区分不同品牌激光打印机墨粉色痕,并可为其他书写材料无损检验鉴定提供借鉴。     

利用RASSF1A位点检测母体血浆中胎儿SNP分型

目的探讨利用母体血浆中高甲基化RASSF1A位点进行胎儿SNP分型的应用价值。方法随机收集10个未孕健康妇女和45例不同孕期（早期5例、中期20例、晚期20例）孕妇的血样本及相应胎儿组织（绒毛组织、羊水、胎盘组织）;利用甲基化敏感限制性内切酶BstUI酶切后进行PCR,产物进行血细胞、血浆和胎儿组织（绒毛或胎盘）DNA RASSF1A序列的甲基化模式检测,并采用直接测序法对SNP rs4688725位点进行分型。结果经BstUI酶消化,RASSF1A序列在母体血细胞中均未检出,而在绒毛或胎盘组织中均能检出;在45名孕妇血浆中,RASSF1A序列均能被检出,且序列内的SNP分型与相应胎儿组织一致;在10名非孕妇女血浆中均未检出RASSF1A序列。结论母体和胎儿DNA中RASSF1A基因启动子区域的甲基化模式存在差异,可用于对母体血浆中的游离胎儿DNA进行SNP分型。     

利用放射性核素骨显像检查鉴别鼻部骨折新旧程度回顾性法医学分析

目的 利用放射性核素骨显像检查鉴别鼻部骨折的新旧程度.方法 对48例疑似陈旧性鼻部骨折伤者放射性核素全身骨显像、局部断层骨显像及CT融合图像在鼻部骨折处放射性分布、浓聚情况的分析,鉴别鼻部骨折的新旧程度.结果 48例中8例放射性核素全身骨显像、局部断层骨显像及CT融合图像在鼻部骨折处显示放射性分布增高、浓聚区,诊为新鲜骨折,评定为轻伤;40例在鼻部骨折处未见明显异常放射性分布增高、浓聚区,诊为陈旧骨折,与本次外伤无因果关系.结论 放射性核素骨显像检查在鉴别鼻部骨折新旧程度方面可得到满意结果.     

A 21‐Locus Autosomal SNP Multiplex and its Application in Forensic Science

To develop a cost‐effective technique for single‐nucleotide polymorphism (SNP) genotyping and improve the efficiency to analyze degraded DNA, we have established a novel multiplex system including 21‐locus autosomal SNPs and amelogenin locus, which was based on allele‐specific amplification (ASA) and universal reporter primers (URP). The target amplicons for each of the 21 SNPs arranged from 63 base pair (bp) to 192 bp. The system was tested in 539 samples from three ethnic groups (Han, Mongolian, and Zhuang population) in China, and the total power of discrimination (TPD) and cumulative probability of exclusion (CPE) were more than 0.99999999 and 0.98, respectively. The system was further validated with forensic samples and full profiles could be achieved from degraded DNA and 63 case‐type samples. In summary, the multiplex system offers an effective technique for individual identification of forensic samples and is much more efficient in the analysis of degraded DNA compared with standard STR typing.     

Fatal Child Abuse Presenting with Multiple Vertebral and Vascular Trauma

This case study involves the unexplained death of a previously healthy 30‐month‐old child. Reportedly, she was found unresponsive by her foster father following a 2‐hour nap. She was transported to the hospital and died in the emergency room of unknown causes. Blood drawn in the ER showed hemoglobin of 4.3 mg/dL. Postmortem skeletal survey, magnetic resonance imaging (MRI) of the brain, and retinal examinations were negative. The medical examiner assumed jurisdiction of the body. The autopsy showed massive retroperitoneal hemorrhage, transections of the abdominal aorta, and inferior vena cava, and complete tears of the anterior longitudinal ligament of the spine at C5/C6 and L1/L2 with diastases of the vertebral bodies at the corresponding intervertebral disk spaces. The case is believed to be extraordinary due to both the extent of injury that does not match the reported history and the mechanism of vertebral and vascular injuries.     

Voices heard and unheard – A Scandinavian perspective

Children's rights to participate in legal processes concerning them have been a key policy issue for the Scandinavian legislators during recent decades. From the 1980s, there have been frequent amendments to the law to secure the position of the child. Despite numerous provisions stating the right of the child to express his or her views and for these to be considered before decisions are taken, there are continuing obstacles to full recognition of children as legal subjects. Too often children's voices are not heard or not heeded. This article explores the reasons for this and argues that the ambition to promote equal parenthood is one reason for the failure to give the child's views real impact on decision-making in matters concerning children.     

论对特殊被害人的刑法保护—以我国刑法第254条、255条之规定为视角

所谓特殊被害人,即我国刑法第254条、255条规定的遭受打击报复、陷害等犯罪行为的控告人、申诉人、批评人、举报人、会计人员、统计人员等六种被害人员。当前,这六种人员在履行职责、维护正义、维护国家、集体、自身利益时,其中的部分人时常遭到打击报复、陷害,人身权利和民主权利得不到保障。造成这一现象的原因固然众多,然而刑法第254条、255条规定之缺陷为重要缘由,因为对特殊被害人的刑法保护在法律上主要体现为刑法第254条、255条之规定。因此,为加强对特殊被害人的刑法保护,尽快完善这两条已成当务之急。     

略论我国第一个《刑法修正案》

笔者认为,《刑法修正案》不属于单行刑法,用“修正案”的形式补充修改刑法具有灵活性、便于适用性,能保持刑法统一等优点,但是我国第一个《刑法修正案》在立法技巧上仍不当地沿用了以往单行刑法的模式。     

‘The beneficent and legal godfather’: a history of the guardianship of unaccompanied immigrant and refugee children in Australia, 1946–1975

Abstract

In Australia in 1946, the Immigration (Guardianship of Children) Act was passed. This Act was intended to support the postwar migration to Australia of British children, unaccompanied by their parents, and provided them a guardian in Australia: the Immigration Minister. This key provision of the Act continues into the present, covering all unaccompanied child migrants, including refugees. Starting with the parliamentary debates which occurred at the formation of the Act in 1946, this article traces a history of the Act until its first High Court challenge in 1975. In doing so, a focus is placed on a series of key questions raised by its production of categories: How does the Act construct ideas of migranthood? What do the discussions it has provoked have to say about notions of parenthood and the ideal family? And, finally, what concepts of the child have been produced through this legislative and legal history? Through an examination of archival materials, parliamentary debates, court records, and newspaper coverage, this article explores the discursive productions of the Act, following the understanding that ideas of the family, of parenthood, of guardianship, of migrant status, and of the child are not natural, but instead are historically created and produced, here through racialized techniques of governmentality.