贵州地区嗜尸蝇类调查

目的　调查贵州地区与尸体有关的蝇类。方法　根据昆虫学调查方法 ,设定调查地域 12个 ,于 2月、 5月、 8月和 11月的 10～ 2 0日采集一次标本。结果　全年 12个地域 44次共采集嗜尸蝇类标本 3 743 3只 ,经鉴定隶属于 5科 17属 2 7种。结论　取得了贵州地区嗜尸蝇种及区域、季节分布的资料 ,填补了在《中国蝇类》中未见贵州报道的空白。为下一步研究各嗜尸蝇种的生活史打下了基础 ,为推测死者的死亡季节及死亡环境提供参考依据。     

与时俱进努力做好新时期消防工作

与时俱进做好消防工作是新时期的新要求。与时俱进做好消防工作 ,就要深入调研 ,把握趋势 ;着眼发展 ,做好规划 ;面对现实 ,提高素质     

Freeze-framing territory: time and its significance in land governance

This paper explores how temporal symbolism is used to construct land as an object of enduring value. Using anthropological and ethnographic sources, I highlight how different practices of landholding are informed by different understandings of “permanence”. I elaborate on how institutions of landholding employ “temporal signposts” to mark out land allocation and access arrangements over time. In conclusion, I explore the political roles of inter-temporal land governance, positing a link between codified “permanences” of landholding in land laws and the entrenchment of socio-spatial power. I suggest that the temporal dimension of land governance demands interrogation alongside the spatial.     

我国举证时效制度之检讨

举证时效制度乃指民事诉讼当事人的举证行为须在规定之期限内完成,若当事人怠于举证将会受到证据失权或费用制裁之惩罚的一项证据规则。其制裁方式主要有二：证据失权与费用制裁,若当事人因故意或重大过失导致举证逾期其可能承担证据失权之后果;若当事人因轻微过失导致逾期,需承担费用之不利益。周全的审前准备程序和答辩失权规则是其存在的基础,完善审前程序与建立答辩失权制度是我国举证时效制度充分发挥效用的保证。     

移植时间和检测方法与allo-HSCT受者的DNA嵌合率及法医学意义

影响allo-HSCT受者DNA嵌合率的因素有很多:如受者的疾病类型、移植前预处理方案、移植时病情的严重程度、造血干细胞来源、局部的GVHD、HLA是否相合等。本文主要讨论与法医学关系最为密切的2个影响因素,即移植后时间和检测方法的灵敏度。法医物证学工作者在检案实践中,对allo-HSCT受者进行亲权鉴定或个体识别时,需注意移植后时间和检测方法的灵敏度对嵌合率的影响。女性受者接受男性供者HSCT的情况下,受者毛囊中可以检出供者Y染色体DNA,检案实践中来源于犯罪现场的生物检材不能仅仅检测Y染色体特异遗传标记,以免导致错误判断。     

Development of a Real‐Time PCR‐Based Method for Analyzing Semen‐Specific Unmethylated DNA Regions and Methylation Status in Aged Body Fluid Stains

The detection of semen in forensic investigation is considered important evidence of sexual assault. In this study, we report the development of a real‐time polymerase chain reaction‐based method for identifying semen that can simply and rapidly analyze the semen‐specific unmethylated region of the DACT1 gene. Using two fluorescent probes designed for the methylated or unmethylated status, this method could perform quantitative analysis of the methylation status in this region. Furthermore, this method was used to analyze various body fluid samples, including 29‐year‐old semen and blood stains. The results showed that this method can detect almost exclusively semen or nonsemen signals even in highly decomposed samples, while a few semen or nonsemen samples showed slight signals of the other fluorescence probe. Although there is still a need for further analysis such as setting thresholds to analyze unknown samples, this method could be a useful supplementary tool for identifying semen, especially in old stains such as those in cold‐case investigations.     

Screening Test for Shed Skin Cells by Measuring the Ratio of Human DNA to Staphylococcus epidermidis DNA

A novel screening method for shed skin cells by detecting Staphylococcus epidermidis (S. epidermidis), which is a resident bacterium on skin, was developed. Staphylococcus epidermidis was detected using real‐time PCR. Staphylococcus epidermidis was detected in all 20 human skin surface samples. Although not present in blood and urine samples, S. epidermidis was detected in 6 of 20 saliva samples, and 5 of 18 semen samples. The ratio of human DNA to S. epidermidisDNA was significantly smaller in human skin surface samples than in saliva and semen samples in which S. epidermidis was detected. Therefore, although skin cells could not be identified by detecting only S. epidermidis, they could be distinguished by measuring the S. epidermidis to human DNA ratio. This method could be applied to casework touch samples, which suggests that it is useful for screening whether skin cells and human DNA are present on potential evidentiary touch samples.     

Application of mRNA Expression Analysis to Human Blood Identification in Degenerated Samples that were False‐negative by Immunochromatography,,

Forensic laboratories are often faced with cases in which methamphetamine hydrochloride‐mixed blood is unable to be identified as human blood by immunochromatography against human hemoglobin A0. The application of mRNA expression analysis to samples that showed a false‐negative with immunochromatography was investigated as an alternative approach that did not depend on the antigen–antibody reaction. Real‐time PCR was used to examine the expression levels of blood markers such as glycophorin A, spectrin beta, and hemoglobin beta. Hemoglobin beta was the only marker that was specifically detected in blood, while glycophorin A was useful for determining human specificity. Hemoglobin beta showed good detection sensitivity and was detectable in 37‐year‐old blood stains. Hemoglobin beta was exclusively detectable in methamphetamine hydrochloride‐mixed blood stains. Detergents and disinfectants did not significantly influence mRNA markers. The proposed mRNA expression analysis was suitable for human blood identification as an alternative method to immunochromatography.     

Cutaneous (tPA) and Skeletal (TnI) mRNA as Markers of Aging in Contused Wound

Wound age estimation is one of the most important forensic aspects. Troponin I (TnI) and many cytokines, for example, tissue plasminogen activator (tPA), are involved in wound inflammation and healing. Skeletal (TnI) and cutaneous (tPA) mRNA was detected using real‐time PCR in 25 female albino rats. They were divided into 5 groups: control and 4 injured groups. Injured groups were sacrificed 1, 6, 24, and 30 h after inflicting contused wound. The expression levels of cutaneous (tPA) were decreased significantly at 1, 6, and 30 h after contusion (71.7%, 30.7 and 16.9%), while the expression levels of skeletal (TnI) were increased significantly at 1 and 6 h post‐traumatic, then they gradually decreased until reaching normal levels at 24 h and assumed significantly lower levels at 30 h postcontusion. These results suggested that the determination of cutaneous (tPA) and skeletal (TnI) mRNA levels was useful for wound age estimation.     

Classification of Opium by UPLC‐Q‐TOF Analysis of Principal and Minor Alkaloids

Opium is the raw material for the production of heroin, and the characterization of opium seizures through laboratory analysis is a valuable tool for law enforcement agencies to trace clandestine opium production and trafficking. In this work, a method for opium profiling based on the relative content of five principal and 14 minor opium alkaloids was developed and validated. UPLC‐Q‐TOF was adopted in alkaloid analysis for its high selectivity and sensitivity, which facilitated the sample preparation and testing. The authentic sample set consisted of 100 “Myanmar” and 45 “Afghanistan” opium seizures; based on the data set of the 19 alkaloid variables in them, a partial least squares discriminant analysis classification model was successfully achieved. Minor alkaloids were found to be vitally important for opium profiling, although combined use of both principal and minor alkaloids resulted in the best geographical classification result. The developed method realized a simple and accurate way to differentiate opium from Myanmar and Afghanistan, which may find wide application in forensic laboratories.