谈残疾人中等职业学校中班主任的爱与严

在残疾人中等职业学校班主任的工作岗位上,如何正确把握对残疾学生＂爱＂与＂严＂的内涵及合理尺度,是一个重要问题。笔者通过自己亲身工作经历阐述了特教班主任对学生的＂爱＂与＂严＂的理解,列举了班主任对学生的＂爱＂与＂严＂的几种不当类型,并对＂爱＂与＂严＂的关系作了简单的论述,以此来明确特教教师对职校学生的＂爱＂与＂严＂所应掌握的程度。     

大学生道德责任教育实效性的现状归因及机制保证

就目前而言,大学生道德责任教育还存在着一些问题。要提高大学生道德责任教育实效性就要建立相应的机制加以保证,如何运用监测预警机制保证大学生道德责任知识的高知晓度;运用政策激励机制确保大学生道德责任知识的认同度;运用效果评估机制反馈与校正大学生道德责任知识的内化度;运用利益导向机制增强大学生道德责任行为意向性;运用调控整合机制保证大学生道德责任行为的一贯性,是文章的主旨。     

单位制研究的“新传统主义”及有关争论

魏昂德的"新传统主义"概念为中国单位制研究在极权主义和多元主义范式之外为提供了一种新的分析视角,其理论前提在于国家高度集中的再分配体制垄断了重要的社会资源,而这成为国家整合和控制单位组织及其成员的手段和工具。分析梳理了学界对"新传统主义"单位制研究的批评及争论,指出单位制研究将面向多元化的具体组织情景,回归到真实的组织运作过程,由此将生发出新的理论增长点。     

The legal framework of private universities in Spain

The setting up of Universities in Spain is considered to be part of the fundamental right to establish private educational institutions. For a long time there have only existed Church Universities alongside Public Universities, and these have had their legal basis in Agreements between Spain and the Vatican. As of 1991 there has been an on-going process of setting up purely private Universities, to the point that there are now eleven besides the Church Universities. This article examines their legal framework from the point of view of their organisation, the system governing their teaching staff and their effective autonomy with regard to the Public Authorities. This revised version was published online in July 2006 with corrections to the Cover Date.     

p33.4位点扩增片段长度多态性及其法医学应用的研究

以聚合酶链反应（PCR）、聚丙烯酰胺凝胶垂直电泳和银染法对中国100名无关个体小卫星区域p33．4位点的扩增片段长度多态性（Amp－FLPs）进行了研究，检出了8个等位基因。通过BIOTRAC系统进行数据处理．各等位基因重复单位的数目分别为7、10到15，其中在13～14之间发现一差值不足一个重复单位长度的罕见等位基因。片段长度分布于603～1115bP之间，基因频率分布于0.5～33．5％间，杂合度为64％，DP值为84．5％。对5个家庭25名相关个体进行分析，符合孟德尔遗传定律；对同一个体不同组织的DNA进行P33.4位点的分型研究表明，该技术适宜于法医物证检验。此外，本研究以Chelex处理不同检材制备DNA模板用于扩增，率先建立了比常规方法更快、更简便、更为实用的检验方法。     

Understanding Sexual Harassment a Little Better Reed and Bull Information Systems Ltd v. Stedman

This case note reviews the guidelines issued by Morison J. in the Employment Appeal Tribunal at the end of the decision in Reed and Bull Information Systems Ltd v. Stedman [1999] I.R.L.R.299. The author argues that while the judge’s decision is to be welcomed in adopting an approach more sympathetic to victims of sexual harassment, it also raises a number of problems by placing a burden on the victim to place the harasser on notice that she does not welcome his conduct. The guidelines are likely to be usefully applied in any jurisdiction that has rules forbidding sexual harassment. The author considers the guidelines from both a practical and a doctrinal angle and indicates that the right to be free from sexual harassment is one that the courts are reluctant to protect like other civil rights. This revised version was published online in July 2006 with corrections to the Cover Date.     

论可持续发展与我国立法体系的重新架构

我国的国情决定了我国必须走可持续发展的道路 ,而作为实现可持续发展重要手段的现行法律与可持续发展的要求差距颇大。为此 ,有必要以可持续发展思想为指导重新架构我国立法体系 ,使之符合可持续发展的要求。     

大鼠心肌早期缺血内皮素的表达

为探讨心肌缺血后内皮素在心肌组织内表达的变化。用免疫组化SABC法研究内皮素的表达情况。结果发现缺血60min后 ,缺血区心肌细胞呈局灶性阳性 ;缺血120min后 ,血管平滑肌、内皮细胞及心肌细胞浆均呈阳性着色。对照和缺血30min组心肌组织未见有阳性反应。上述各组HE染色未见明显病理改变。     

Business 2.0: Kyrgyz middlemen in Guangzhou

ABSTRACT

Among the many ‘businesspeople’ whom the promise of commercial success has drawn to southern China in recent years one can find a small number of Kyrgyz middlemen. Working mostly with Russian-speaking clients, their job is to organize buying trips, coordinate with local manufacturers, translate, and oversee cargo shipments. Based on ethnographic fieldwork since 2013, this article examines in detail the careers, work routines and business model adopted by Kyrgyz middlemen in Guangzhou. I argue that in contrast to the early bazaar or shuttle traders, who have been operating across Eurasia since the 1990s, these Kyrgyz middlemen constitute a next kind of economic actor within more diversified, service-oriented and formalized value chains across post-Socialist Eurasia (referred to here as Business 2.0). One of these middlemen’s most salient contributions is to translate between the informal and formal domains of national economies as well as within cross-border economic transactions.     

Quantitation of human genomic DNA through amplification of the amelogenin locus

An alternate method for quantitation of human genomic DNA is presented. Quantitative template amplification technology (abbreviated "Q-TAT") estimates the quantity of human DNA present in an extract by comparing fluorescence in X and Y amplicons produced from unknowns with fluorescence in a standard curve amplified from known quantities of reference DNA. Q-TAT utilizes PCR and electrophoresis with fluorescent detection/quantitation, precluding the need for new instrumentation, methodology, or quality assurance associated with slot-blot or real-time PCR. In a comparison study incorporating shared samples, Q-TAT was found to be more sensitive than widely used slot-blot methods but somewhat less sensitive than real-time PCR. Among samples containing DNA concentrations ranging from 100 pg/microL to 2-4 ng/microL, Q-TAT produced DNA concentration estimates that agreed reasonably well with either Quantiblot or real-time PCR. Q-TAT was reproducible with a typical coincidence of variation of about 35%. Quantitation of human DNA in this study involved summing fluorescence in X and Y amplicons in unknowns and quantitation standards. However, analyzing fluorescence in X and Y amplicons individually could allow estimates of male and female DNA present in mixtures to be made. Moreover, since X and Y amplicons exhibit sizes of 210 and 216 bp, respectively, the integrity as well as the concentration of the genomic DNA template can be assessed. Q-TAT represents an alternate method useful for the quantitation of human genomic DNA prior to amplification of STR loci used for identity testing purposes. The method uses existing equipment and procedures in conjunction with a well-characterized DNA standard to produce concentration estimates for unknowns that reliably produce STR profiles suitable for analysis.