不同培养液对牛孤雌生殖胚胎和体细胞核移植胚胎体外发育的影响

采用体外成熟的牛卵母细胞 ,经 5 μmol/LIonomycin 5min和 2mmol/L 6 DMAP 5 μg/mLCCB 4h激活 ,分别在TALP M 199(TM ,1∶2 )、BMOC M 199(BM ,1∶2 )和CR1aa培养液内培养 ,2 4h和 3 6h各组卵母细胞的卵裂率分别为5 6.4%、5 5 .6%、5 3 .6%和 72 .0 %、75 .9%、79.0 % ,无显著差异 (P >0 .0 5 ) ;培养 8d后囊胚发育率分别为 18.2 %、2 1.4%和 2 9.3 % ,CR1aa内培养孤雌生殖胚的囊胚发育率显著高于TM (P <0 .0 1)。皮肤成纤维细胞构建的重组胚经同法激活后 ,分别在TM、BM和CR1aa内培养 ,2 4h和 3 6hBM和CR1aa内重组胚的卵裂率显著高于TM (4 9.7%、49.8%比 2 0 .5 % ;74.1%、73 .2 %比 5 0 .0 % ) (P <0 .0 1) ;虽然培养 8d后各组胚胎的囊胚发育率无显著差异 ,但CR1aa内重组胚的囊胚发育率稍高 (12 .3 %比 9.8%、11.5 % )。结果表明 ,CR1aa更适合孤雌生殖和体细胞核移植胚胎的体外培养。     

辽宁警专教育技术工作谈

教育技术对深化教育改革、推进素质教育,有着重要的意义。用教育技术取代电化教育是教育发展的必然结果。教育技术是一项系统工程,需要学校各部门协同作战。学校的全体教师、管理干部和电教人员,对搞好教育技术工作都有着义不容辞的责任。     

俄印关系的发展及其特点

苏联解体后,俄印关系经历了三个阶段:从一度降温到关系改善,再到建立战略伙伴关系。这是两国不断调整各自的对外政策以适应冷战后国际形势发展的结果。俄印关系的主要特点是:不再含有军事同盟性质;防务合作是重点;合作领域扩大。     

Violence,fear, and development in Latin America: a critical overview

This introduction presents the core concepts that shape this special issue on the impact of violence and the processes of development in Central and South America. The understanding of development is considered in terms broader than the economic context alone, in order to assess wider social and political aspects. With a similarly expansive scope, forms of violence are addressed that range from direct physical harm and bodily attack to the often more subtle aggression of racialised abuse or the pressures on community-centred production from dominant market forces. In these contexts, violence, economic initiatives, and political allegiances form unintended and often dangerous networks of consequence for development matters. All the articles in this volume exemplify further the spatial environments of violence and diverse ‘landscapes of fear’ that shape our existence and help to define our actions, territories, and understanding of what happens around us.     

健全和完善公民表达权的法律保障机制

党的十七大报告把"表达权"列为公民四权之一,并强调要依法保障,这对我国推进社会主义民主法治、构建和谐社会以及大力发展文化创新都具有非常重要的意义。然而,现行法律、法规以及司法资源对"表达权"的保护相对不足,因此,需要进一步健全和完善公民表达权的法律保障机制。     

Social capital

In parallel with, and as a complement to, globalisation, ‘social capital’ has enjoyed a meteoric rise across the social sciences over the last two decades. Not surprisingly, it has been particularly prominent across development studies, not least through heavy promotion by the World Bank. As a concept, though, as has been argued persistently by a minority critical literature, social capital is fundamentally flawed. Although capable of addressing almost anything designated as social, it has tended to neglect the state, class, power, and conflict. As a buzzword, it has heavily constrained the currently progressive departure from the extremes of neo-liberalism and post-modernism at a time of extremely aggressive assault by economics imperialism. Social capital should not be ignored but contested – and rejected.     

血浆中芬太尼的检测

本文概括阐述了近10年国内外检测血浆中芬太尼的基本情况。对各种检测方法及分离净化过程中应用的各种萃取方法进行了对比和分析。在此基础上提出我国药物动力学研究及公安司法工作对检测血浆中芬太尼的新要求。     

Citizenship: a perverse confluence

This article discusses the different meanings that citizenship has assumed in Latin America in the past few decades. Its main argument is that, in the perverse confluence between neo-liberal and democratic participatory projects, the common reference to citizenship, used by different political actors, projects an apparent homogeneity, obscuring differences and diluting the conflict between those projects.     

区际民事案件移送制度初探

区际民事案件移送制度的作用主要是为了节约司法资源、减低司法成本、提高司法效率、保证司法公正、缓和区际管辖权冲突、避免当事人挑选法院、保护当事人合法的诉讼权益和促进区际司法协助等。美国和澳大利亚区际民事案件移送制度的立法与实践对我国具有一定的借鉴意义。     

论环境与资源诉讼中的公益理念

环境权从某种意义上属“社会性”权利 ,无论在立法还是司法实践中 ,这一权利都带有明显的公益色彩。为保障公民环境权的实现 ,我国应借鉴西方国家经验 ,放宽对环境诉讼起诉资格的限制 ,对现行司法制度进行制度创新