2011～2012年中国—东盟货物贸易数量分析与预测

2011年中国与东盟的进出口贸易总额突破3600亿美元,增幅回落但仍在高位,中方逆差扩大且呈进一步向海关特殊监管区域、外商投资企业、资源类产品以及东部沿海省市等方面集中的趋势。一般贸易、外资企业、机电产品等方面均占中国—东盟贸易的一半。私营企业对双方进出口增长的贡献突出,而加工贸易、外商投资企业、国有企业等方面所占比重则在下降。数据检验分析显示,中国、日本、韩国、美国与东盟的贸易具有稳定的协整关系、关联程度。计量预测2012年的中国—东盟经贸合作将保持持续的增长势头。     

关税递减的福利效应及其实证分析——CEPA货物贸易零关税的一种验证

随着区域经济合作的不断加强,有关区域关税递减的福利影响日益受到关注.以中国CEPA货物贸易零关税制度的实施为研究对象,考虑在一定的假设条件下,忽略贸易转移,而供给曲线向上倾斜的情况,从理论上分析关税壁垒消除带来的福利效应,并从实证上对其验证.首先从关税损失和增值税创造角度分析税收收入所受的影响;其次引入数量指数和价格指数,计算各部分的损失和收益,近似给出福利影响的数量值.测算结果更直观的表明零关税制度导致了内地税收的损失和福利损失,但相对整体进口情况来看,其影响非常有限;但部分行业的港产品进口的增长带来的冲击需要进一步关注.这些直观的量化结果可以为区域再进一步建立自由贸易区提供参考,可以为产业结构的调整和优化提供依据.     

论犯罪定量因素立法化对法定刑模式的要求——以抢劫罪为实例的研究

在中国刑法规定中,不但规定犯罪的基本行为,同时规定犯罪成立的量的要件,后者即犯罪定量因素立法化。这种立法模式有积极价值,也有消极影响,甚至带来多罪刑阶段加重减轻事由与法定刑之间的不协调问题。要解决有关问题,应该在法定刑上运用多罪刑阶段之间的交叉式规定模式,并在立法技术上体现出立法公正的要求。     

心理尸检的历史与现状

心理尸检作为一种定性研究和定量研究相结合的方法，在国外已经广泛应用于各相关领域，然而在中国，尤其是在中国内地，心理尸检的研究还处于起步阶段。本文整合了国内外一些心理尸检的研究新进展．分析了心理尸检的实证研究、信度、效度以及访谈上存在的一些问题，并提出今后该领域的发展方向．以期尽早把心理尸检的方法应用于各相关领域中。     

NET框架在量化系统设计中的应用

以开发边防士官学校学员量化系统为例，介绍了在．NET框架下进行系统设计并采用了．NET＋SQLServ-er2008技术构建方式。最后以主要实现技术方面阐述了系统的需求分析、系统主要功能、主要实现技术等方面。经试用验证后，证明了此系统达到了设计的要求。     

Optimization of DNA recovery from toothbrushes

In human identification, the victim's toothbrush is an invaluable personal item as the deposited cellular material contains DNA from which a reference profile can be produced. The profile obtained then allows direct comparison to be made with the profile from the unidentified body. This study was undertaken to determine the minimum number of bristle bundles that would generate a complete DNA profile. The minimum period of usage for a toothbrush to retain enough cells for genotyping was also investigated. We also tested two commonly used DNA extraction methods: QIAamp^® DNA Mini Kit and Chelex^® 100 to explore the efficiency of these protocols in recovering DNA from toothbrushes. In this experiment, volunteers brushed their teeth for 1, 7, 14, or 30 days. DNA was extracted from 5 and 10 bundles of bristles cut from the collected toothbrushes. The amount of DNA recovered was quantified by quantitative real-time PCR, and DNA genotyping was performed for each sample. Data revealed that QIAamp^® DNA Mini Kit performed better at yielding DNA in terms of purity, quantity, and quality than Chelex^® 100. It was also found that, with a suitable method of recovery, DNA samples from five bundles of bristles from all of the toothbrushes generated complete profiles. Based on the experimental results, a general guideline concerning the appropriate extraction method and the quantity of the starting material for the analysis of DNA from toothbrushes could be suggested.     

关于提请罪犯减刑、假释的几点思考

监狱建立对罪犯有悔改表现的考核机制,在决定对罪犯提请减刑、假释时,应依据罪犯悔改表现程度适时对罪犯进行综合评价,进而决定是否提请减刑、假释。使监狱减刑、假释工作更加公开、公平、公正。     

Validation study of endogenous reference genes for normalization of quantitative real time PCR data in post mortem skin tissue

Gene expression profiling may provide insights into the molecular mechanisms underlying wound skin repair contributing to applications in the field of post mortem forensic wound age estimation. Quantitative real-time PCR (qPCR) is the most sensitive technique for gene expression studies but represents a complex procedure with several requirements for accurate data analysis. One of the major pre-requisites is the selection and validation of appropriate endogenous control genes for normalization in a given set of samples. In this study, the gene expression stabilities of ten endogenous controls ACTß, ß2M, PPIA, GAPDH, HPRT1, PGK1, SDHA, TBP, UBC, and YWHAZ were evaluated in two sets of samples: post mortem human wounded and unwounded skin tissue aimed for subsequent wound age estimation studies. The most stable genes were determined by calculating a gene expression normalization factor for each sample based on the geometric mean of the ten selected genes using the reference gene validation software, geNorm. In uninjured skin, the most stable endogenous controls were YWAZ, with the highest stability, followed by PGK1 and GAPDH. In the wounded samples, GAPDH was the most stable gene followed by PGK1, ACTß and HPRT1. Both groups revealed some differences relative to reference gene expression stabilities, nevertheless, PGK1 and GAPDH could serve as two possible common reference genes for qPCR data normalization as both were identified as stable genes in wounded and unwounded skin tissues. The differences of expression stabilities of the reference genes identified in both groups support the need for accurate normalization of gene-expression levels previous to qPCR studies.     

Packed column chromatography, high-resolution gas-chromatography and high pressure liquid chromatography in comparison for the analysis of cannabis constituents

The identification and the quantitative estimation of cannabis constituents is important for forensic purposes. High resolution gas-chromatography gives better results than gas-liquid chromatography with packed columns, as it shows a better resolution and higher number of constituents. Different quantitative values were found with the two chromatographic procedures. High-pressure liquid chromatography revealed the presence of cannabinoid acids in fresh cut influorescences of cannabis plants. The ratio acid/neutral cannabinoid may be useful in supplying information about the age of the cannabis preparations.     

UV中LogA-λ谱图法与A-λ谱图法的比较及应用

本文通过logA-λ谱图法与A-γ谱图法的比较,介绍了logA-γ谱图法在刑事毒物分析中具有测试浓度范围宽,灵敏度高,定性比对直观,定量精确、方便等优点.并经临案实践证明,值得同行借鉴.