考古样品中Amelogenin同源基因的提取和检测

目的利用人类性染色体Amelogenin同源基因在X、Y染色体上序列长度的差异,选择设计引物,对考古样本进行古DNA性别信息研究。方法采用苯酚/氯仿-二氧化硅-超滤离心方法提取东岭墓葬群殉人骨骼、牙齿古DNA,PCR扩增,非变性聚丙烯酰胺凝胶电泳(PAGE)分离和检测古DNA扩增片段。结果8个墓葬16个样品中有7个样品出现阳性扩增检测,目标基因片段清楚,男性为二条带(X、Y),女性为一条带(X),牙齿样品检测成功率优于骨骼样品。结论改进的苯酚/氯仿—二氧化硅—超滤分离法是较好的古DNA提取方法,具有降低PCR抑制剂、消耗成本低和提取成功率高等特点。基于人类X、Y染色体Amelogenin同源基因的古DNA性别分析方法可成为分子考古重要的技术方法。     

历史上中国东西部经济发展不平衡的形成及其原因

春秋战国至秦汉,关中经济区和巴蜀经济区并称"天府之国",在我国西部呈南北纵向排列,成为两颗耀眼的明珠,是西部经济发展最为辉煌的时期.汉晋以后,中国经济重心逐渐南移,其实这也是我国经济重心自西向东的转移.唐代有"扬一益二"之说,扬州和益州成为两颗耀眼的明珠.它们呈东西横向排列,表明中国经济发展西不如东.历史的发展证明,当今的西部大开发是中国经济格局的大变动,必须作为一项长期战略,树立全局意识和观念,切实解决开发中的深层次问题.     

主体性思想的形成与发展

主体性思想是传统西方哲学的一个基本思想,它萌发于古希腊哲学,主要探讨世界及其本质;它在近代得以确立,进入认知主体性阶段;它在现代得以发展,以人的生存为基础,又被称为生命主体性思想。     

中国古代史研究21世纪展望--"21世纪中国历史学展望学术讨论会"中国古代史组讨论纪实

2002年5月云南大学召开了"21世纪中国历史学展望学术讨论会",这是进入21世纪以后中国历史学界的一次盛会。与会专家学者对20世纪的中国历史学研究进行总结,畅谈了对21世纪中国历史学的展望。中国古代史组的专家学者们在研讨中,提出一些重要的学术见解和改革建议。这对今后中国古代史的学科发展乃至整个中国历史学的发展,都将具有重要意义。     

Muslim identity in the Balkans before the establishment of nation states

The background of the contemporary Macedonian “antiquization” can be found in the nineteenth century and the myth of ancient descent among Orthodox Slavic speakers in Macedonia, adopted partially due to Greek cultural inputs. The idea of Ancient Macedonian nationhood has also been included in the national mythology during the Yugoslav era. An additional factor for its preservation has been the influence of the Macedonian Diaspora. After independence, attempts to use myth of ancient descent had to be abandoned due to political pressure by Greece. Contemporary antiquization on the other hand, has been revived as an efficient tool for political mobilization. It is manifested as a belated invention and mass-production of tradition, carried out through the creation of new ceremonies, interventions in the public space and dissemination of mythological and metaphysical narratives on the origin of the nation. There have also been attempts to scientifically rationalize claims to ancient nationhood. On the political level, the process of antiquization reinforced the political primacy of its promoters, the ruling Internal Macedonian Revolutionary Organization – Democratic Party for Macedonian National Unity (VMRO-DPMNE), but had a negative impact on the interethnic relations and the international position of the country.     

简论江南古民居的保护与开发——以湖州衣裳街周宅、王宅为例

近年来各级政府加大对江南古民居的保护与开发力度,湖州衣裳街的周宅、王宅得到应有的重视与保护,取得可喜成绩。但也应看到,在对古民居的保护与开发中还存在许多亟待解决的问题。必须制订完善规划,采取切实可行的相关措施,才能对江南古民居进行充分的保护与开发。     

‘Colonizin’ in reverse

Abstract

This is a revised version of a paper presented on 22 June 2006 by Kenneth Parker at The English Academy of Southern Africa, University of the Witwatersrand, Johannesburg, on the occasion of the presentation of a Gold Medal to Professor Colin Gardner for distinguished service to English. The paper takes a tongue-in-cheek look at ‘Britishness’ and conforming to British standards – including what the British saw as the ‘way forward for the English language’. It shows how the erstwhile ‘colonized’, and more particularly their British-born descendants, have been doing some ‘colonizin’ of their own.     

New Dreams Bloom on the Ancient Silk Road

正In September 2013,Chinese President Xi Jinping paid state visits to four countries in Central Asia.While in Kazakhstan,President Xi proposed that China join hands with Central Asian countries to build a Silk Road economic belt–again turning the world spotlight onto that ancient trade artery connecting Asia and Europe.The     

Wanyao——An Ancient Village Riding the Tides of Time

The region used to be the main base in southern Zhejiang for the production of everyday blue and white porcelain.Today,it retains its ancient style with timehonored residences,kilns,temples and opera stages.     

The Conviction of Dr. Crippen: New Forensic Findings in a Century‐Old Murder

Abstract: Dr. Hawley Crippen was accused and convicted of murdering his wife in London in 1910. Key to the conviction was microscopic analysis of remains found in the Crippen’s coal cellar, which were identified as Cora Crippen based on a scar she was said to have. Dr. Crippen was hanged, always proclaiming his innocence. In this study, genealogical research was used to locate maternal relatives of Cora Crippen, and their mitochondrial haplotypes were determined. Next, one of the pathology slides of the scar was obtained, DNA was isolated, and the haplotype was determined. That process was then repeated. Finally, both DNA isolates were assayed for repetitive elements on autosomes and repetitive elements specific to the Y chromosome. Based on the genealogical and mitochondrial DNA research, the tissue on the pathology slide used to convict Dr. Crippen was not that of Cora Crippen. Moreover, that tissue was male in origin.