电针激活Nrf2/HO-1通路抗帕金森病模型小鼠氧化应激的机制研究

目的 观察电针“风府”“太冲”“足三里”穴对帕金森病(Parkinson’s disease, PD)模型小鼠大脑纹状体中酪氨酸羟化酶(tyrosine hydroxylase, TH)、核因子E2相关因子2(nuclear factor-erythroid 2-related factor 2,Nrf2)、血红素加氧酶-1(heme oxygenase-1,HO-1)水平的影响,探讨电针治疗PD的可能机制。方法 将30只小鼠按随机数字表法分为对照组、模型组和电针组,每组10只。模型组、电针组小鼠按照30 mg/kg腹腔注射1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine, MPTP)制备PD小鼠模型。电针组小鼠给予“风府”“太冲”“足三里”穴电针干预,每日1次,每次30 min,连续干预12 d。其余两组小鼠不予任何干预。采用爬杆实验和悬挂实验观察3组小鼠行为学改变,旷场实验观察小鼠自主运动总路程,免疫组织化学法检测小鼠大脑纹状体中TH表达水平,实时荧光定量PCR法检测小鼠大脑纹状体中Nrf2、超氧...     

基于色度、有效成分相关性的蒲黄炒炭识别模式

目的 研究不同炮制程度蒲黄炭颜色与有效成分含量的相关性,为蒲黄炭质量控制提供参考。方法 利用色差仪对不同炮制程度蒲黄炭进行色度测定,依据色度值对炒炭程度进行判别分析;建立高效液相指纹图谱并测定生蒲黄及不同炮制程度蒲黄炭中香蒲新苷和异鼠李素-3-O-新橙皮苷的含量;采用化学计量方法对有效成分与色度值进行Spearman相关性分析。结果 蒲黄药材指纹图谱共标定17个共有峰,并指认了香蒲新苷和异鼠李素-3-O-新橙皮苷两种成分。不同炮制程度的蒲黄炭色度值具有显著差异,并且所建立的判别函数具有统计学意义（P＜0.05）,可通过Fisher线性判别函数区分生品、轻炭、标炭、重炭4类。香蒲新苷、异鼠李素-3-O-新橙皮苷与色度值L、b、Eab成显著正相关（P＜0.05）,与a值无相关性（P＞0.05）。结论 高效液相色谱结合色度分析技术可用于蒲黄炭的质量评价,其通过内在、外在质量控制相结合的方法可客观、有效地评价蒲黄炭的质量。     

弱势群体社会资本缺失的因素分析

社会资本缺失既是弱势群体的重要特征,又是其弱势地位形成的重要因素。弱势群体的社会资本缺失主要表现在资本因素、结构因素、文化因素、制度因素及组织因素等几方面。     

Analysis of sensitivity and specificity: precise recognition of neutrophils during regeneration of contused skeletal muscle in rats

In this report, we applied the TissueFAXS 200 digital pathological analysis system to rapidly and accurately identify neutrophils during regeneration of contused skeletal muscle, and to provide information for follow-up studies on neutrophils to estimate wound age. Rat injury model was established, and skeletal muscle samples were obtained from the control group and contusion groups at 1, 1.5, 2, 3, 4, and 6 h, as well as at 1, 3, 5, and 15 d post-injury (n = 5 per group). The expression of nuclei and neutrophils was detected by hematoxylin and eosin (HE) staining and immunohistochemical (IHC) staining. A total of 20 injury site areas of 0.25 mm² (0.5 mm × 0.5 mm) were then randomly selected at all time points. A TissueFAXS 200 digital pathological analysis system was used to identify the positive and negative numbers. Knowledge of five professional medical workers were considered the gold standard to measure the false positive rate (FPR), false negative rate (FNR), sensitivity, specificity, and area under the curve (AUC) of receiver operating characteristic (ROC) curves. As a result, with a staining area of neutrophils from 8 µm² to 15 µm², the FPR was 4.28%–12.14%, the FNR was 12.42%–64.08%, the sensitivity was 35.92%–87.58%, the specificity was 87.86%–95.72%, the Youden index was 0.316–0.754, the accuracy was 82.80%–88.30%, and the AUC was 0.771–0.826. The AUC was largest when the cut-off value of the staining area was 12 µm². Our results show that this software-based method is more accurate than the human eye in evaluating neutrophil infiltration. Based on the sensitivity and specificity, neutrophils can be accurately identified during regeneration of contused skeletal muscle. The TissueFAXS 200 digital pathological analysis system can also be used to optimize conditions for different cell types under various injury conditions to determine the optimal cut-off value of the staining area and provide optimal conditions for further study. Furthermore, it will provide evidence for forensic pathology cases.     

Fingerprint analysis for the determination of hand origin (right/left) using the axis slant in whorl patterns

Fingerprints are frequently encountered during both civil and criminal investigations. Fingerprints possess numerous characteristics that assist with personal identification. Determining the hand of origin (right or left) for an individual fingerprint would help reduce investigation time and potentially eliminate certain suspects. In this study, we collected a total of 2 900 single digit fingerprints from 290 individuals, and the whorl axis slant was examined in the 743 whorl pattern fingerprints (385 from the right hand and 358 from the left hand). A slant towards the right side was present in 81.82% of samples from the right hand, while a slant towards the left side was observed in 80.73% of samples from the left hand. After applying a chi-square test to the dataset, the results were found to be statistically significant for the determination of hand origin. Our results suggest that the whorl axis slant in a fingerprint is indicative of hand origin (right or left).

Key points

• Single digit fingerprints with whorl pattern were analyzed.
• Whorl “axis slant” was used to determine the hand origin.
• Right axis slant would indicate the right hand of the print.
• Left axis slant would indicate the left hand of the print.

     

委托理财协议中的保底条款效力之追问

保底条款是委托理财协议中最重要的条款,它不仅决定了这种投资方式的存在与否,同时亦是委托理财失败诉争时当事双方相持不下的根本原因。对于该条款的效力认定,目前的法律与司法实践并没有提供能真正能起到指导与评价作用的答案。因此,从合同的私法性、社会法性及保底条款与其它条款的勾连角度来追问保底条款的效力问题是合理与适法判决的关键所在。     

我国劳动关系研究的历史回顾与未来展望：基于1949—2021年的文献分析

从历史维度回顾我国的劳动关系研究,总结具有中国特色的劳动关系管理经验,对于指导中国和谐劳动关系的构建具有重要意义。研究基于我国不同阶段的发展目标对劳动关系的影响,将1949—2021年我国劳动关系的研究划分为5个阶段,并进行分阶段的可视化分析。在此基础上,从政府、企业和劳动者3个劳动关系核心主体回顾每个阶段的研究主题,梳理我国劳动关系研究的演进过程。本研究提出巩固中国共产党在劳动关系工作中的领导地位、探究政府构建和谐劳动关系的路径、挖掘企业社会责任履行的方式等未来我国劳动关系的主要研究命题,构建了中国情境下劳动关系的研究模型并提出了劳动关系实践的发展方向。     

甘肃省民间融资发展存在的问题及成因分析

民间融资在全国范围内存在共性问题,甘肃省的民间融资现状更存在特殊性问题。制约甘肃省民间融资发展的实质原因是民间融资自身特性特殊,相关国家立法及地方立法体系不完善,政府监管无力等因素。文章在分析民间融资存在的问题上,提出解决问题的对策,引导和规范民间金融的发展。     

Participatory budgets in Canoas (Brazil) and Cascais (Portugal). A comparative analysis of the drivers of success

ABSTRACT

This article is a comparative analysis of two internationally awarded cases of Participatory Budgets in Canoas (Brazil) and Cascais (Portugal). Our main argument is that the success of these PBs relies on political/administrative, societal, and geographic drivers. This conceptual model has been adopted to analyse and discuss the success of the two case studies. The provision of an original conceptual model for comparative analysis and the empirical knowledge from the two cities are supported by the review of main literature in this field of study, and aim to contribute, with original findings, to the international debate on participatory budgeting.     

The legitimization and institutionalization of ‘parental alienation’ in the Province of Quebec

ABSTRACT

This article examines the legitimization and institutionalization of ‘parental alienation’ discourse in the Province of Quebec, Canada. It draws upon an analysis of 31 documents (legislation, research reports and articles, training documents, professional documents and media articles) and interviews with 13 key informants, who were selected based on their knowledge of ‘parental alienation’ in research, policies or practices. The research findings reveal that the legitimization and institutionalization of ‘parental alienation’ discourse is a more recent process than in other provinces and countries, but that it has now permeated child custody as well as child protection proceedings. Academic researchers and media have been instrumental in this legitimization and institutionalization process, while the role played by changes to child protection policies is more ambiguous. The findings reveal researchers’ and experts’ tendency to distance themselves from Gardner’s controversial work on ‘parental alienation syndrome’ and to address the critiques by proposing new approaches and new concepts. However, the terms ‘parental alienation syndrome’, ‘parental alienation’ and ‘alienating behaviours’ are often used interchangeably, and assessment practices tend to rely on similar indicators.