Developmental Validation of the Quantifiler® Duo DNA Quantification Kit for Simultaneous Quantification of Total Human and Human Male DNA and Detection of PCR Inhibitors in Biological Samples*

Abstract: The Quantifiler^® Duo DNA Quantification kit enables simultaneous quantification of human DNA and human male DNA as well as detection of inhibitors of PCR in a single real-time PCR well. Pooled human male genomic DNA is used to generate standard curves for both human (ribonuclease P RNA component H1) and human male (sex determining region Y) specific targets. A shift in the cycle threshold (C_T) values for the internal positive control monitors the presence of PCR inhibitors in a sample. The assay is human specific and exhibits a high dynamic range from 0.023 to 50 ng/μL. In addition, the multiplex assay can detect as little as 25 pg/μL of human male DNA in the presence of a 1000-fold excess of human female DNA. The multiplex assay provides assessment of the DNA extract and guidance for the selection of the appropriate AmpFℓSTR^® Amplification Kit to obtain interpretable short tandem repeat profiles.     

SEX EDUCATION LEFT AT THE THRESHOLD OF THE SCHOOL DOOR: STRICTER REQUIREMENTS FOR PARENTAL OPT‐OUT PROVISIONS

Current state law creates the risk that, if sex education is not provided to a child in public school, no similar instruction will be given to the child. Legislatively enacted opt‐out provisions give parents broad control over their child's education by granting them the option to have their child excused from any sex education requirements within a public school's curriculum. Through public school sex education, professionals provide youth information aimed at protecting them from the dangers of pregnancy and sexually transmitted diseases (STDs). A stricter statutory opt‐out provision should be enacted by state legislatures that only allows parents to excuse their child from sex education after a parent attends a 90‐minute STD prevention course and receives the instructional material used in the school's sex education curriculum. Parents should be provided up‐to‐date information and a structural framework designed by the school to encourage them to discuss with their child the many sexual issues addressed within a school's sex education curriculum.     

Performance of the supervised learning algorithms in sex estimation of the proximal femur: A comparative study in contemporary Egyptian and Turkish samples

Sex estimation standards are population specific however, we argue that machine learning techniques (ML) may enhance the biological sex determination on trans-population application. Linear discriminant analysis (LDA) versus nine ML including quadratic discriminant analysis (QDA), support vector machine (SVM), Decision Tree (DT), Gaussian process (GPC), Naïve Bayesian (NBC), K-Nearest Neighbor (KNN), Random Forest (RFM) and Adaptive boosting (Adaboost) were compared. The experiments involve two contemporary populations: Turkish (n = 300) and Egyptian populations (n = 100) for training and validation, respectively. Base models were calibrated using isotonic and sigmoid calibration schemes. Results were analyzed at posterior probabilities (pp) thresholds >0.95 and >0.80. At pp = 0.5, ML algorithms yielded comparable accuracies in the training (90% to 97%) and test sets (81% to 88%) which are not modified after employing the calibration techniques. At pp >0.95, the raw RFM, LDA, QDA, and SVM models have shown the best performance however, calibration techniques improved the performance of various classifier especially NBC and Adaboost. By contrast, the performance of GPC, KNN, QDA models worsened by calibration. RFM has shown the best performance among all models at both thresholds whereas LDA benefited the best from using both calibration methods at pp >0.80. Complex ML models are not necessarily achieving better performance metrics. LDA and QDA remain the fastest and simplest classifiers. We demonstrated the capability of enhancing sex estimation using ML on an independent population sample however, differences in the underlying probability distribution generated by models were detected which warranted more cautious application by forensic practitioners.     

论我国未成年犯罪人社区矫正的必要性与可行性

针对未成年人犯罪的成因及特点,除了对一部分社会危害性大的未成年犯罪人实行监禁矫治而外,对那些确实有悔改表现,不再危害社会的未成年犯罪人,社会能够对其进行有效控制的实行社区矫治,这种做法不仅震慑了犯罪,同时也挽救了一大批确有悔罪表现的失足青少年,使他们重新步入社会,开始新的生活。有效地对未成年犯罪人进行社区矫治不仅具有现实意义而且具有战略意义,本文仅就实行未成年犯罪人社区矫正的必要性与可行性加以探讨。     

Rethinking Timing of First Sex and Delinquency

The relation between timing of first sex and later delinquency was examined using a genetically informed sample of 534 same-sex twin pairs from the National Longitudinal Study of Adolescent Health, who were assessed at three time points over a 7-year interval. Genetic and environmental differences between families were found to account for the association between earlier age at first sex and increases in delinquency. After controlling for these genetic and environmental confounds using a quasi-experimental design, earlier age at first sex predicted lower levels of delinquency in early adulthood. The current study is contrasted with previous research with non-genetically informative samples, including Armour and Haynie (2007, Journal of Youth and Adolescence, 36, 141–152). Results suggest a more nuanced perspective on the meaning and consequences of adolescent sexuality than is commonly put forth in the literature.

To Flirt or Not to Flirt? Sexual Power at the Bargaining Table

We begin by exploring the lay belief that women can use flirtation to their advantage in professional contexts and contrast it with trained negotiators' negative views on flirtation. We then examine the impact of flirtation on negotiators' impression formation. We explore whether a flirtatious style aids women in the trade-off they often face between perceived likability and perceived competence. We discover both an upside and a downside to flirting at the bargaining table. Although flirtation appears to be positively related to women's likability, negotiators who flirted were judged to be less authentic than those who refrained from exercising their sexual power.     

完善我国未成年犯社区矫正制度的思考

我国的未成年犯社区矫正还处在摸索阶段，许多制度尚未完善，需要根据我国的具体国情以及借鉴外国的先进经验，从矫正执行主体、矫正项目、立法等三个方面入手，探索完善我国未成年犯社区矫正制度。     

Sex differences in the experience of child sexual victimization

This study analyzed 390 (303 girls and 87 boys) cases of child sexual abuse to understand sex differences in child sexual victimization. Analyses focused on three domains that have received considerable empirical attention: victims' relationships with the offender, type and extent of abuse, and disclosure. Findings largely replicated previous efforts that reported sex differences in terms of victims' relationships with offenders. Although results also replicated previous findings about sex differences in disclosure patterns, these sex differences were minor in comparison with similarities in the way most instances of abuse were eventually identified. In terms of type and extent of abuse, results were the opposite of previous findings: girls, not boys, were the victims of more physical injury, violent threats, and use of force; and girls, not boys, were more likely to have physically resisted abuse. Divergences in findings were interpreted in terms of memory biases and social forces operating differently on the sexes.