早期心肌缺血心肌细胞凋亡的实验研究

探讨早期心肌缺血心肌细胞凋亡的意义。采用DNA缺口末端标记法 (TUNEL)对大鼠实验性心肌缺血早期 (6h内 )不同时间缺血损伤区心肌细胞凋亡的情况进行观察。结果 :缺血 1h在缺血区域发现少数散在的凋亡阳性细胞 ,3h达高峰 ,随后下降。正常区域未发现凋亡细胞。缺血边缘区域也在缺血 1h局部开始出现心肌细胞凋亡 ,并随缺血时间延长心肌细胞凋亡指数增加 ,缺血 5h达高峰。表明凋亡是早期缺血性心肌细胞损伤的主要方式 ,心肌凋亡检测可望为早期心肌梗死的死后诊断提供一个灵敏客观的新方法。     

PCR扩增SON基因3’非编码区进行种属鉴定

根据人 DNA的 SON基因碱基序列选择性设计一对特异性引物 ,并对人和猕猴、阿拉伯狒狒、猪、牛、羊、马、驴、骡、狗、猫、兔、大白鼠、小白鼠、豚鼠等 14种哺乳类动物染色体 DNA的 SON基因 3’非编码区中的种属特异性区域进行 PCR扩增 ,扩增产物经 SSCP分离银染色技术检测 ,观察到人和 14种哺乳类动物的 SSCP图谱有明显不同。本方法可以对人和上述 14种哺乳类动物进行种属鉴定 ,适合于法医学种属鉴定的应用     

明代南京官署志概说

中国的志书不仅源远流长 ,而且种类繁多。除了记载各级行政区的地方志外 ,还有大量记录某一特定区域内某项或某方面内容的专志。然而 ,几乎所有研究志书的学者都忽略了一种记载政府某部门典章故事的可称之为“官署志”的专志。这种官署志的编印在明代达到一个新的高峰 ,而且多集中在留都南京。本文在回顾官署志起源的基础上 ,集中讨论明代南都官署志的编撰特点、内容及其史料价值     

胶体金免疫层析一步法快速检测G1m(3)因子

建立一种简便快速的胶体金免疫层析一步法 ,用于检测 G1m(3)因子。采用柠檬酸三钠还原法制备胶体金颗粒 ,标记抗人 G1m(3)单克隆抗体 ,研制出抗人 G1m(3)因子免疫层析检测试剂盒。样品的 G1m(3)因子 ,与测试条上的金标记抗体结合后沿着反应膜移动 ,再与膜上固相抗体结合形成肉眼可见的红色反应带。使用该方法可检出 10万倍稀释的血清样品 ,整个试验只需 5 min完成。对常见的 2 3种动物血 (痕 )检验 ,未出现交叉反应。在 10 0例样品的检测中 ,本方法的检测结果 ,与 Dot- EL ISA的检测结果的符合率为 10 0 %。该方法适用于法医物证快速检验。     

杀虫双的检验和质谱解析

本文用气相色谱/质谱联用方法检验杀虫双,并解析杀虫双的质谱图.     

Sustainability Reporting by Australian Universities

There is continuing debate about the consequences of climate change and an increasing emphasis placed on the disclosure of social and environmental indicators by organisations. Universities have been traditionally known as places where intellectual inquiry and advances in knowledge are paramount. Therefore, there is an expectation that universities would place much emphasis on sustainability reporting to fulfil the needs of a range of stakeholders. The objective of this study is to investigate the nature and extent of sustainability disclosures reported by Australian universities in stand‐alone sustainability reports. The findings suggest significant differences in the nature and extent of sustainability disclosures. This study has implications for policy‐makers and university administrators and is a call to senior university leaders to take up the challenge and contribute to the global conversation with regard to sustainability reporting.     

Assessing the credibility of how climate adaptation aid projects are categorised

This article presents the findings of a re-evaluation of all 5,200 aid projects that OECD donors reported for 2012 as “climate change adaptation”-related, based on the “Rio marker” classification system. The findings confirm those from the academic and grey literature that the absence of independent quality control makes the adaptation Rio marker data almost entirely unreliable. This lack of credibility impedes meaningful assessments of progress toward the mainstreaming of adaptation in development cooperation activities. It also erodes trust in international climate negotiations, given that these data are frequently used in the financial reporting of developed countries to the UNFCCC.     

Promoting country ownership and inclusive growth? An assessment of Feed the Future

This article investigates the level of country ownership and inclusivity in Feed the Future (FtF) projects in Ethiopia, Ghana, Haiti, Senegal, and Tanzania. It finds that though interventions largely align with country-defined priorities and plans, other aspects of country ownership – consultation and provision of aid through local systems – are weaker than expected. Regarding inclusion, FtF is to some extent exclusive, perhaps as an unintended consequence of alignment with national development plans that do not reasonably promote equity. Focus is mostly on productive areas and ‘market-ready’ smallholders. The article suggests policy actions needed to better achieve expected results.     

Which aid initiatives strengthen food security? Lessons from Uttar Pradesh

This study contributes to existing research on the relative effectiveness of aid initiatives in addressing food insecurity in India. Specifically, it compares the effects of crop, livestock, credit, and WASH interventions implemented by a development NGO with the impacts of a public direct-transfer scheme on food security among communities in Uttar Pradesh. The NGO’s agricultural and WASH interventions and the government’s Antyodaya ration-card scheme appear to have the largest positive influence. Their positive impact can be enhanced by the presence of good governance and by the simultaneous application of several beneficial initiatives.