高职电子商务教学改革浅析与人才定位思考

在高职类院校,电子商务专业教学目标的模糊性和学生就业的盲目性成为阻碍电子商务专业发展的屏障。要打破这种屏障就要从电子商务教学改革和人才定位入手,构建以培养学生能力,以实践教学为核心,提高就业率为导向的模块化课程教学体系。并且,高职院校还应该根据社会需求,重点培养网站策划和运营、网络广告策划和推广、网上贸易平台应用、网络营销等方面的人才。从而更好地改善该专业学生的学习模式。使高职院校电子商务专业人才定位更为清晰。     

贵州地区汉族Penta D和Penta E基因座遗传多态性

有关Penta D和PentaE基因座遗传多态性调查,国内外文献已有多篇,但尚无对我国贵州地区的遗传学数据报道,为此本文进行了调查,现报道如下。     

Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples

An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized beyond the six previously developed by our laboratory for a total of 26 non-CODIS miniSTR markers. These new markers produce short PCR products in the target range of 50-150 base pairs (bp) by moving the primer sequences as close as possible-often directly next to the identified repeat region. These candidate loci were initially screened based on their small amplicon sizes and locations on chromosomes currently unoccupied by the 13 CODIS STR loci or at least 50 Mb away from them on the same chromosome. They were sequenced and evaluated across more than 600 samples, and their population statistics were determined. The heterozygosities of the new loci were compared with those of the 13 CODIS loci and all were found to be comparable. Only five of the new loci had lower values than the CODIS loci; however, all of these were much smaller in size. This data suggests that these 26 miniSTR loci will serve as useful complements to the CODIS loci to aid in the forensic analysis of degraded DNA, as well as missing persons work and parentage testing with limited next-of-kin reference samples.     

Gender Mainstreaming Strategies in the E.U.: Delivering on an Agenda?

Recent years have witnessed the emergence of anew policy style within the E.U., characterized by voluntary policy transfer between member states and soft policy instruments including exchange of best practice, targets, benchmarking and national league tables. This article examines how these methods have been used by gender mainstreaming advocates and evaluates the impact of this strategy to-date upon E.U. policy-making procedures and outputs. It is argued that mainstreaming has provided new opportunities for feminists to influence the E.U. policy agenda, but that the impact of mainstreaming varies between sectors and member states. The concluding section considers the implications of E.U. mainstreaming from the perspective of the European Women's Lobby(E.W.L.). This discussion highlights the potential opportunities and risks for feminists of mainstreaming. This revised version was published online in July 2006 with corrections to the Cover Date.     

培养学生素质 促进终身体育——对我院女生健美操学习情况的调查与分析

对我院 8个班健美操教学现状及女生参加健美操学习的心理趋向的抽样调查与分析结果表明 ;多数女生喜欢健美操项目 ;在教学中应重视素质培养 ,养成学生长期主动锻炼的习惯 ,形成终身体育的能力。     

中国与欧盟反倾销法程序性规则比较及借鉴

本文全面分析了欧盟反倾销法及中国《反倾销条例》程序性规则的基本内容及特点 ,通过比较分析 ,论述了我国反倾销程序性立法的差距和不足。欧盟反倾销法程序性规则较为系统完备 ,我们应取其精华 ,完善我国的反倾销立法。     

冤狱是怎样炼成的——从《窦娥冤》中的举证责任谈起

窦娥的悲剧固然反映了人类认识能力的有限,以及科技水平的不够发达,但是其更深层的原因则在于无罪推定原则的缺失,以及相应的权力约束机制的缺席。无罪推定原则的功能不仅表现在举证责任的分配方面,而且表现在以权利约束权力的制衡方面。因此,现代刑事诉讼制度的完善,仍然应当着眼于基本制度的建设,而与科技基本无关。     

苦地胆内酯类化合物的制备及其体外抑菌效果研究

为探讨中药苦地胆有效成分苦地胆内酯的抗菌效果,用浓度为800mL/L的乙醇溶液进行热回流浸提,制备苦地胆内酯的粗提物,经硅胶柱层析分离、纯化,再结晶,制得苦地胆内酯类化合物,用薄层层析法进行苦地胆内酯检测与鉴定,并进行了苦地胆内酯类化合物的体外抑菌试验。结果显示,当浓度为2.0mg/mL时,苦地胆内酯类化合物对巴氏杆菌、蜡状芽孢杆菌具有明显的抑制作用(P0.05),对金黄色葡萄球菌、肠炎沙门氏菌和鸡大肠杆菌呈现极明显的抑制作用(P0.01);浓度分别为50、10mg/mL时,苦地胆内酯类化合物对5种供试菌均有极明显的抑制作用(P0.01)。苦地胆内酯类化合物对供试金黄色葡萄球菌、肠炎沙门氏菌、巴氏杆菌、鸡大肠杆菌、蜡状芽孢杆菌的最小抑菌浓度分别为0.313、0.313、0.156、0.625mg/mL和1.25mg/mL;对蜡状芽孢杆菌的最小杀菌浓度为2.5mg/mL,对其他4种供试菌的最小杀菌浓度均为0.625mg/mL。表明,苦地胆内酯类化合物具有很好的体外抗菌效果。     

青海牦牛病毒性腹泻病毒的分离与鉴定

采集青海省泽库县某牧户疑似牦牛病毒性腹泻病牛的样品,将RT-PCR检测为阳性的样品处理后接种MDBK细胞,并盲传至第10代,检测每一代细胞中牛病毒性腹泻病毒的E0基因。通过分离株接种细胞后产生的病变效应观察、免疫荧光试验、电镜观察、RT-PCR扩增以及序列分析鉴定了该病毒。结果表明,盲传的每代细胞中均可检测到E0基因;接毒后的细胞在盲传至第7代时出现明显的细胞病变;免疫荧光试验中能观察到细胞内发出的特异性荧光;经浓缩、纯化的病毒在电镜下呈直径为40～60nm的病毒粒子,将其命名为QHZK株。对克隆的E0基因测序后提交至GenBank(登录号:JF927789),并将获得的序列与其他国内外分离株的E0序列比对,同源性为73.6%～98.2%;系统进化分析表明该分离株属于牛病毒性腹泻病毒1b亚型。