基于钟基因Clock和Bmal1调控探究辰时针刺自发性高血压大鼠的降压机制

目的 以自发性高血压大鼠（spontaneous hypertensive rat,SHR）为观察对象,探究针刺降压的钟基因调控作用。方法 将24只SHR随机分为针刺组和模型组,12只Wistar-Kyoto（WKY）大鼠作为正常组。针刺组选择辰时针刺SHR双侧曲池、足三里穴,模型组和正常组仅接受与针刺组同等强度的捆绑操作。4周后,各组分别在辰、酉时随机抽取6只大鼠,测量鼠尾收缩压（systolic blood pressure, SBP）和舒张压（diastolic blood pressure, DBP）,然后检测血清5-羟色胺（5-hydroxytryptamine,5-HT）、褪黑素（melatonin, MT）的含量以及心脏生物钟基因Clock和Bmal1的表达水平。结果 分组因素对SBP、DBP、血清MT含量、Clock表达结果的RQ值、Bmal1表达结果的RQ值的主效应均有统计学意义（P＜0.05）,时辰因素对各指标的主效应和两者的交互作用均无统计学意义（P＞0.05）。模型组辰、酉两个时辰的SBP和DBP均高于正常组（P<0.05）,针刺组辰时的SBP和DBP及酉时的SBP均低于模型组（P<0.05）。模型组辰时MT含量较正常组降低,5-HT含量较正常组升高（P<0.05）,针刺组辰时MT含量较模型组升高,5-HT含量较模型组降低（P<0.05）;针刺组酉时的MT含量较模型组升高（P<0.05）。针刺组辰时心脏Clock基因表达水平和酉时心脏Bmal1基因表达水平较模型组升高（P＜0．05）。结论 辰时针刺可以降低SHR的“双峰”血压,其作用机制可能与提高血清MT含量,减少5-HT含量以及提高心脏Clock和Bmal1基因的表达水平有关。     

TGF-β1在SCN5A基因变异所致心源性猝死中调控作用

SCN5A基因变异所致心源性猝死发生机制尚不清楚。目前研究显示TGF-β1介导心肌纤维化以及离子通道重构调节机制的异常可能是SCN5A基因变异导致SUNDS发生的主要原因。本文就转化生长因子β1对SCN5A基因变异所致心源性猝死调控的影响机制研究进展进行综述,以期为心源性猝死法医学研究和实践提供参考。     

G20机制非正式性的起源

与国际货币基金组织等正式国际组织不同,G20是布雷顿森林体系内一种新的非正式对话机制.已有的关于G20机制起源的研究,都较多关注了G20机制诞生的必然性,却忽视了G20为什么会采取非正式机制形式的问题.本文通过对G20机制诞生历史的考察,分析了G20机制非正式性的形成过程,并指出,非正式性是G20缔造者关于新机制形式的一个基本设想,也是对当时历史经验的总结.理解G20机制非正式性的形成对于我们把握G20究竟是一种什么类型的国际机制以及如何处理G20与其他多边国际机制的关系具有一定的启发意义.     

山羊痘病毒RPO30基因绿色荧光蛋白质粒的构建及融合表达

为了研究针对山羊痘病毒RPO30的siRNAs的干扰效果,构建了RPO30基因与增强型绿色荧光蛋白(EGFP)基因的融合表达质粒,并在BHK-21细胞中表达.通过对RPO30基因进行PCR扩增,克隆入表达载体pEGFP-N1,并进行双酶切及测序鉴定;将阳性重组质粒转染BHK-21细胞,检测绿色荧光蛋白的表达和RPO30...     

口蹄疫病毒3C基因与增强型绿色荧光蛋白基因的真核共表达

为验证siRNAs对口蹄疫病毒(FMDV)复制的抑制效果,用反转录聚合酶链反应(RT-PCR)扩增Asia 1型口蹄疫病毒Jiangsu/China/2005株的3C基因,克隆入增强型绿色荧光蛋白(EGFP)表达载体pEGFP-N1中,并进行双酶切、PCR及测序鉴定。将阳性重组质粒转染PK-15细胞,检测EGFP的表达和3C基因转录水平。结果显示,经PCR及双酶切鉴定,目的基因片段大小与预期相符,测序结果与Jiangsu/China/2005株相应序列一致。荧光显微镜和流式细胞仪均检测到细胞内有EGFP表达,实时荧光定量PCR检测到细胞内有3C基因的转录。证实,成功构建了FMDV 3C基因与EGFP共表达质粒并在PK-15细胞中获得了表达。     

结核分枝杆菌pdhA基因的原核表达及其免疫原性分析

以结核分枝杆菌H37Rv菌株的基因组DNA为模板,采用PCR方法扩增出1 100bp的pdhA(pyruvate dehydrogenase E1component alpha subunit)基因,经限制性酶切后,与pET-28a载体连接,转化到大肠杆菌BL21中,重组菌经1mmol/L IPTG诱导表达了pdhA蛋白,并用Ni-His-resin纯化了目的蛋白,经Western-blot分析鉴定了目的蛋白的免疫原性。结果显示,重组质粒的双酶切鉴定和DNA测序均正确;SDS-PAGE和Western-blot分析结果显示,在44ku处获得一目的条带,且具有很好的免疫原性。本研究成功获得了高纯度的重组蛋白pdhA,为深入研究其功能奠定了基础。     

山羊关节炎-脑炎病毒陕西分离株全基因组的克隆和序列分析

为了解山羊关节炎-脑炎病毒(CAEV)陕西分离株全基因组的特点及基因演化变化,根据Gen-Bank中登录的CAEV CO株的全基因组序列,设计合成了5对引物,对CAEV陕西株的全基因组进行了PCR扩增,并对扩增产物进行了克隆和测序。结果显示,CAEV陕西株的基因组全长为9 065nt,获得的GenBank登录号为GU120138,包括两端的长末端重复序列,结构蛋白编码基因gag、pol、env和调节蛋白编码基因tat和rev,以及辅助蛋白编码基因vif。核苷酸序列比对表明,CAEV陕西株和CAEV甘肃株的同源性为99.6%,与CAEV CO株的同源性为92.4%,根据全基因组序列和gag基因序列构建的遗传进化分析结果显示,CAEV陕西分离株属于SRLV B2亚型。     

Employment preservation vs. creditors’ repayment under bankruptcy law: The French dilemma?

The paper investigates the French dilemma associated with court administered resolution of corporate financial distress. In such a legal system, the courts seek a double objective: maintaining job positions through continuation, and determining the best outcome for the claimants. We discuss this dilemma empirically, using a unique sample of bankruptcy files on French SMEs. We address successively three critical questions. First, we highlight the determinants of the final bankruptcy outcome (continuation through reorganization or sale, or piecemeal liquidation): does continuation (the most employment-friendly outcome) depend on the firm's characteristics, and/or on the way the procedure is managed? Second, we study the determinants of the creditors’ recovery rates: do the courts play an active role in increasing recoveries? Third, we address the dilemma directly by focusing on sales as a going concern. We model the court administered selection process between rival buyout offers: do the courts balance the social content against the financial content of each offer? Is there an explicit arbitrage between employment preservation and creditor recoveries? Our main results are: (1) the French courts actively work to facilitate continuation against liquidation, and thus play a role in employment preservation. Besides, we find continuation is more likely to prevail when default is an outcome of specific difficulties (outlets, finance, and production). (2) We confirm the Radulovic (2008) findings: the global recovery rate mainly depends on the firm's ex ante characteristics at the time of triggering, while the way the procedure is managed by the court has little impact. Similarly to LoPucki and Doherty (2007), continuation via reorganization does not generate lower recovery rates on average than the other outcomes. (3) Last, the courts’ choice between rival buyout offers confirms that social considerations prevail in the arbitration. Yet, the courts still consider financial issues as well (a higher sale price increases the chances that an offer is selected), but without clear connection with the amount of due claims (one direct consequence is a moderate recovery rate on sales).     

Webcasters’ protection under copyright – A comparative study

This paper studies the fourth generation ‘peer-2-peer’ (P2P) file sharing technology which is also known as streaming technology from the technology point of view. It critically analyses the nature of streaming technology and assesses similarities to broadcasting for the purpose of copyright protection in the name of webcasting. In addition, it considers the possibility of protecting webcasting within the framework of broadcasting under copyright and the extent of broadcasters’ rights applicable to webcasters. Based on the conclusions derived from the discussion of UK, New Zealand, Italian and US law on streaming, WIPO Standing Committee on Copyright and Related Rights (SCCR) draft documents will be critically analyzed. The Last part of this work will offer suggestions as to how to protect webcasting (streaming technology) under the umbrella of copyright.