Detection of pretreated fingerprint fluorescence using an LED-based excitation system

Abstract:  Optimization of a light emitting diode (LED)-based excitation system for the detection of pretreated fingerprint fluorescence is described. Fluorescent ridges can usually be excited by irradiation with forensic light sources such as xenon arc lamps or quartz-halogen lamps with high-power output and suitable filters. However, they are too expensive for many crime laboratories in smaller organizations. We concentrated on LEDs which have advantages over conventional light sources in that they are simpler and of lower cost, but the power output and quality of each individual LED unit is not sufficient for the detection of weak fluorescent ridges. To resolve this subject, blue and green LED arrays composed of ninety LED units were adopted and suitable low pass filters for them were designed. An experimental system, consisting of blue and green LED arrays with the suitable low pass filters for illumination, high pass filters for viewing, a digital camera and a computer, was tested. The fluorescent images of cyanoacrylate ester fumed/rhodamine 6G stained fingerprint on white polyethylene sheet and weak fluorescent ridges of ninhydrin/indium chloride treated fingerprint on white paper were successfully detected and photographed. It was shown that the improvement of LED beam in intensity and quality can compensate the disadvantages, resulting in well-contrasted images.     

The Group of 77 in the international climate negotiations: recent developments and future directions

First, we describe and analyze the main set of G77 positions in the climate negotiations and the dynamics behind the emergence of these positions. While it is puzzling that the G77 has managed to maintain itself as a group in spite of internal differences along variables as prosperity, emissions and vulnerability to climate change, we claim that a core element behind this cohesion is that these countries share domestic governance problems as much as poverty and economic underdevelopment. Second, we discuss how recent trends of economic and political development in the third world influence the climate policy strategies of the G77 group in the future. The main factor here is the economicand social progress in states like China, India and Brazil, which separates them from the poorer and less powerful G77 states. Increasing heterogeneity along variables like governance, growth, and importance for the international economy is creating an increasing drive among the most successful G77 states towards bilateral agreements with industrialised powers. We do not foresee a departure from traditional G77 positions and membership by these states in the official climate negotiations or a departure from the Kyoto process, but an increasing reliance on bilateral agreements with industrialized countries that link considerations for energy security and the environment. The ability to gain these advantages without commitments may make these states less interested in adopting commitments for the post-Kyoto period. This is unfortunate for the LDCs and the AOSIS groups within the G77, who probably are most vulnerable to climate change.

2010年韩国外交综述

过去的2010年,韩国政府在巩固＂新亚洲外交＂成果的基础上,努力应对朝鲜半岛的紧张局势,以举办G20峰会为契机,加强韩美同盟、积极开展经济外交,推动FTA战略的实施,拓展与其他国家的交流合作,促进经济的较快发展。本文旨在探讨韩国政府过去一年的外交政策,对其外交的主要特点进行阐述和分析。     

Cold War brotherhood contested: KATUSAs,slicky boys,American G.I.s,and the Status of Forces Agreement in post-armistice South Korea, 1954–1966

ABSTRACT

As the largest contingent of Americans in postwar South Korea, the G.I. best represented the United States’ Cold War objectives. Their deployment was an emblem of hard power containment, but the G.I. also embodied soft power integration, and through both, G.I.s helped to promote Pax Americana. This article focuses on the militarized masculinity of these ambassadors of America and their people-to-people diplomacy in South Korea between 1954 and 1966. These American G.I.s constructed their militarized masculinity vis-à-vis the Korean male Other, their “lesser” counterparts – the hapless houseboy, the inferior partner soldier, and the menacing slicky boy. At the same time, this liberal imperialism did not go uncontested. Violent imaginaries of the American G.I. from the borderlands were used by Koreans to demand a new bilateral framework – the Status of Forces Agreement in 1966 – to replace the outmoded wartime extraterritorial jurisdiction wielded by the American military after cessation of hostilities on the Korean peninsula in 1953. The militarized masculinity practiced in everyday encounters, thus, became the basis of a critique of American liberal imperialism in one of the United States closest Cold War “brother” nations.     

XPG基因表达用于法医学年龄推断

目的探讨着色性干皮病G组(xeroderma pigmentosum group G,XPG)基因在不同年龄段健康汉族人群中的表达情况,分析XPG mRNA和蛋白表达量与年龄之间的相关性,以期为法医学年龄推断提供新的分子生物学指标。方法收集150名不同年龄段健康汉族人的外周血样,采用TRIzol法提取外周血单个核细胞(peripheral blood mononuclear cell,PBMC)总RNA,通过实时荧光定量PCR检测XPG mRNA在PBMC的相对表达量,酶联免疫吸附试验检测XPG蛋白在血浆中的表达量。结果 XPG mRNA及其蛋白表达量在≤18岁组与19~45岁组之间、≤18岁组与≥46岁组之间的差异均有统计学意义(P0.05),但19~45岁组与≥46岁组之间的差异无统计学意义(P0.05)。XPG mRNA和蛋白表达量均无性别差异(P0.05)。结论 XPG mRNA在PBMC的相对表达量在低龄段内随年龄增加而下降,其血浆中蛋白随年龄增加而升高;XPG基因有望成为法医学年龄推断的新型指标之一。     

Britain’s Place in the Contemporary World

This article offers a personal view of Britain’s place in the contemporary world, analysed in terms of five interlocking rings: the traditional three, i.e. relations with the United States, with Europe and the Commonwealth, to which are added the concepts of process and soft power.     

猪细小病毒YK株NS1基因的克隆与序列分析

提取猪细小病毒(PPV)YK株基因组DNA,利用PCR技术扩增得到了NS1基因全序列,将其克隆到pMD18-T质粒载体并进行了序列测定和分析。结果表明,NS1基因全长1989bp,编码662个氨基酸。氨基酸序列中含有在PPV复制过程中起重要作用的保守基序GKRN,并有3个潜在的糖基化位点NISN、NFSN和NLTR。PPVYK株的NS1基因与其他PPV毒株NADL-2(4973)株、NADL-2(5075)株、NADL-2(5034)株和Kresse株相比,核苷酸同源性分别为98.3%、99.9%、99.9%和99.7%,氨基酸同源性分别为99.7%、99.7%、99.7%和97.7%。结果说明,NS1基因具有高度保守性。     

传染性腔上囊炎疫苗免疫鸡TLR7基因表达的动态变化

给11日龄雏鸡分别口服接种鸡传染性腔上囊炎(IBD)弱毒疫苗(法贝灵,IBD-Blen)1和3头份后,于不同时间采集脾和腔上囊组织,分离纯化淋巴细胞,常规方法抽提总RNA,以鸡β-actin基因为内参,采用半定量RT-PCR法检测两种组织中鸡Toll样受体7(ChTLR7)基因的表达动态。结果显示,接种IBD弱毒疫苗前,试验鸡脾和腔上囊组织中均有ChTLR7的微量表达,疫苗接种后第7 h,脾中ChTLR7 mRNA的表达开始显著上调,至接种后第12 h达峰值,此后迅速下降,至第130 h时降至疫苗接种前的水平;而腔上囊组织中ChTLR7 mRNA的表达自接种后第12 h开始增加,第24 h时达峰值,随后迅速下降,约72 h后恢复至疫苗接种前水平。表明ChTLR7可能参与了IBDV感染早期的天然免疫反应过程。     

浙江畲族人群9个STR基因座的遗传多态性

目的调查浙江畲族人群STR基因座的遗传多态性,为群体遗传学和法医学个人识别、亲子鉴定提供基础数据。方法采用AmpFlSTRProfilerPlus试剂盒(ABI公司),ABI310基因自动分析仪对浙江畲族120名无关个体血样的D3S1358、VWA、FGA、D8S1179、D21S11、D18S51、D5S818、D13S317和D7S820等9个STR基因座进行等位基因频率和基因型频率调查。结果获得浙江畲族人群9个STR基因座的基因频率分布资料,所有基因座基因型频率分布均符合Hardy—Weinberg平衡,计算得杂合度(H)0.655～0.960,个人识别率(DP)0.878～0.960,非父排除率(PE)0.363～0.677,多态信息总量(PIC)0.68～0.86。结论该9个STR基因座在浙江畲族人群中呈高度多态性,在法医学及人类遗传学研究中具有重要意义。     

猪传染性胃肠炎病毒S基因A抗原位点序列的克隆与原核表达

将RT-PCR扩增的猪传染性胃肠炎病毒(TGEV)S基因A抗原位点目的片段克隆、双酶切后连接于表达载体,经酶切、PCR和测序鉴定的阳性重组质粒转化BL21(DE3),用IPTG诱导,进行SDS-PAGE和Western-blotting分析,确定目的蛋白的原核表达情况和免疫特异性。结果显示,目的片段的大小为534bp,序列分析表明,该基因与其他TGEV相应基因具有很高的同源性;Western-blotting检测可见分子质量约43 ku的融合蛋白条带,表明,该蛋白具有良好的反应原性。