猪霍乱沙门氏菌C78-1株ΔcrpΔasd缺失株平衡致死载体系统的构建及生物学特性研究

为开发减毒猪霍乱沙门氏菌的活疫苗载体,通过自杀性质粒介导的细菌同源重组技术,构建了猪霍乱沙门氏菌C78-1株ΔcrpΔasd双基因缺失株,并对其生物学特性进行探讨。首先构建含有缺失1 488bp asd基因的重组自杀性质粒pREΔasd,然后与已构建完成的ΔcrpC78-1缺失株进行接合转移,采用两步法筛选无抗性的C78-1的ΔcrpΔasd双缺失株。PCR鉴定结果表明,C78-1的ΔcrpΔasd双缺失株构建成功;进一步研究表明,该缺失株的生长需要外源的二氨基庚二酸(DAP),且能稳定遗传缺失的asd基因,与C78-1相比,其血清型未发生变化,但其生长速度明显减慢。ΔcrpΔasd C78-1双缺失株可接受asd+质粒作为宿主平衡致死系统高效稳定地表达外源基因,为开发以C78-1为载体的口服多价疫苗奠定了基础。     

H. G. Wells's The Time Machine and the “Odd Consequence” of Progress

The Time Machine of H. G. Wells stands as one of the foundational works of science fiction. It is a true product of its time, a reflection of the fast pace of technological development and social change that makes a romance of the ideology of advancement for its own sake. But between the lines of its “adventure” plot, we find evidence of another, more complex story being told. Wells's work is plagued by ambivalence about the outcome of social and scientific progress—the selfsame progress that gave birth to the time machine in the first place. The Time Machine, in short, is preoccupied by an anxiety about a catastrophe just around the corner, not one born of global war or political turmoil but rather of the absence of turmoil, of need, of struggle. The idea and possibility of fiction itself becomes entangled in this problem of the everyday catastrophe, as it nearly suffocates under the conditions of the utopian society which it creates.     

LED多波段光源显现人民币上潜指印的研究

目的研究显现、提取遗留在人民币上潜在指印的方法。方法采用荧光试剂罗丹明6G对人民币进行染色处理,以LED多波段光源输出的波长为520nm的绿光照射检材,透过580nm的带通型干涉滤光镜观察指印的显现效果,并拍照提取。结果获得亮纹线暗背景的指印图像,指印纹线清晰,反差良好,特征丰富,具备良好的检验鉴定条件。结论 LED多波段光源输出的波长为520nm的绿光与罗丹明6G染色指印的吸收特性吻合,是显现、提取遗留在人民币上潜在指印的良好光源。     

Economic strain and perceptions of social cohesion in Europe: Does institutional trust matter?

The degree to which different social groups get along is a key indicator of the cohesiveness of a society. This study examines perceptions of social cohesion among Europeans and explains variations in those perceptions by considering the separate and combined effects of economic strain and institutional trust. Analyses were conducted with the 27 member countries of the EU based on the Eurobarometer 74.1 on poverty and social exclusion conducted in 2010. Results show that individuals living in households experiencing economic strain perceive social cohesion to be weaker than their less economically hard‐pressed counterparts. By contrast, individuals trusting their political institutions perceived there to be higher levels of cohesion. Furthermore, institutional trust substantially moderates the negative relationship between economic strain and perceptions of cohesion. These results are robust to various model specifications. Moreover, extending the analysis revealed that this moderating effect held when considering social relations between the poor and rich and between different racial and ethnic groups. Theoretical and practical implications of the results are discussed.     

Keine Unsicherheitseinrede des Beschäftigers im Konkurs des Arbeitskräfteüberlassers

Bei Arbeitskr?fteüberlassung kann der Besch?ftiger im Konkurs des überlassers den Entgeltforderungen des Masseverwalters nicht mit der Unsicherheitseinrede begegnen, wenn ein Anspruch auf eine (im funktionellen Synallagma stehende) Gegenleistung nicht mehr besteht. Dies gilt ungeachtet seiner m?glichen Haftung aus § 14 AüG.     

Durchsetzung von Rechnungslegungsansprüchen gegen den verwaltenden schlichten Miteigentümer im Außerstreitverfahren nach § 838a ABGB

In das Au?erstreitverfahren nach § 838a ABGB idF FamErbR?G f?llt seit 1. 1. 2005 die Durchsetzung der mit der gemeinschaftlichen Verwaltung und Benützung "unmittelbar zusammenh?ngenden Rechte und Pflichten" der Miteigentümer, somit jedenfalls die Auseinandersetzungen nach den §§ 833 bis 838 ABGB. Dazu z?hlen daher Rechnungslegungsansprüche gem § 837 ABGB gegen einen Miteigentümer, der die gemeinschaftliche Verwaltung mit allen Rechten und Pflichten eines Machthabers iSd §§ 1002ff ABGB besorgt. Zur Umdeutung einer Klage in einen verfahrenseinleitenden au?erstreitigen Antrag bei Zweifeln über die richtige Verfahrensart gem § 40a JN.     

单克隆抗体酶联免疫吸附抑制试验测定精斑G2m（n）因子

人类免疫球蛋白同种异型(human immunoglobulin allotypes)包括Gm、Km及Am等系统.在法医学实践中可用于个人识别和亲子鉴定.关于人精液中同种异型因子的检测报道极少,我们应用抗G2m(n)因子的检测国内外均未见报道,我     

What Mr. Spock told the earthlings: the aims of political philosophy,action-guidingness and fact-dependency

Abstract

Recently, G. A. Cohen introduced an influential distinction between fact-sensitive and fact-insensitive principles arguing that all basic normative principles are of the latter type. David Miller rejects this claim submitting that the validity of basic normative political principles depends on some general propositions about human nature and societies; for example, that men’s generosity is ‘confined’ and that nature has made ‘scanty provision’ for his wants. Miller ties this view of the nature of basic political principles to the view that political philosophy ought to guide people engaged in real-world politics and claims plausibly that in order to fulfil this purpose, political philosophy must be informed by social science. I argue that Miller neither succeeds in showing that basic principles can be fact-sensitive, nor establishes any connection between the Cohen-Miller disagreement on fact-sensitivity, on the one hand, and the nature and aims of political philosophy, on the other hand.     

荣格的情结理论与犯罪动机

深刻理解犯罪动机是犯罪侦查和犯罪防控的需要，而对犯罪动机的深入研究有赖于刑事实践和科学理论，尤其是心理学的重要成果作基础。作为荣格分析心理学基石之一的情结理论，在犯罪动机研究中拥有理论和实践的双重价值。因而，从情结角度考察犯罪动机，对理解犯罪动机的结构、类型及其发展变化具有重要的理论价值和实际应用价值。     

新城疫疫苗毒与野毒的区别诊断及其多功能检测软件的开发

运用生物信息学软件对GenBank中收录的30株新城疫病毒(NDV)全基因组序列间的差异和它们对应的F基因片段22～420核苷酸序列间的差异进行了分析。发现这些差异高度相关(r=0.937),该片段可作为区分NDV野毒和疫苗毒的指纹序列。据此设计了1对扩增用简并引物(其中一条用于PCR产物的直接测序),建立了RT-PCR-测序技术,并测得2个参数:NDV标准疫苗指纹序列库和指纹序列的变异参数。在此基础上,开发出多功能自动分析软件,通过对该指纹序列的分析,不仅可以区分NDV疫苗毒和野毒,还能同步测定这些病毒的毒力和基因型。该技术对NDV标准毒株的测定结果与已知信息完全吻合,且只需3 d即可获得结果。对不同禽类(鹅、鸽、鸵鸟、鸡)中分离的NDV的测定结果表明,59%为残留的疫苗毒。