Automated processing of forensic casework samples using robotic workstations equipped with nondisposable tips: contamination prevention

An automated process has been developed for the analysis of forensic casework samples using TECAN Genesis RSP 150/8 or Freedom EVO liquid handling workstations equipped exclusively with nondisposable tips. Robot tip cleaning routines have been incorporated strategically within the DNA extraction process as well as at the end of each session. Alternative options were examined for cleaning the tips and different strategies were employed to verify cross-contamination. A 2% sodium hypochlorite wash (1/5th dilution of the 10.8% commercial bleach stock) proved to be the best overall approach for preventing cross-contamination of samples processed using our automated protocol. The bleach wash steps do not adversely impact the short tandem repeat (STR) profiles developed from DNA extracted robotically and allow for major cost savings through the implementation of fixed tips. We have demonstrated that robotic workstations equipped with fixed pipette tips can be used with confidence with properly designed tip washing routines to process casework samples using an adapted magnetic bead extraction protocol.     

The Politics of Technology Decline: Discursive Struggles over Coal Phase‐Out in the UK

The decline of carbon intensive technologies is a key element in the ongoing energy transition and our attempts to tackle climate change. At the same time, our understanding of technology decline and of the associated policies and politics is still limited. This paper builds on the sustainability transitions perspective, a novel approach to analyze socio‐technical transformation, including the complex interplay of policy and technology change. We study the decline of coal‐fired power generation in the United Kingdom from 2000 to 2017 by analyzing the discourse in The Guardian. We find scientists and environmental NGOs criticizing coal for climate and health reasons. Government and incumbent businesses tried to re‐legitimate coal but eventually, their resistance collapsed and coal was almost completely abandoned in just a few years. Particularly devastating for coal were failed promises around carbon capture and storage, rapid diffusion of wind energy, and pressure from various policies. This study contributes to better understanding the contested nature of decline, and the interplay of discursive struggles, technology change, and public policy in sustainability transitions.     

Household Costs and Resistance to Germany's Energy Transition

Germany is an exemplary case of an energy transition from nuclear energy and fossil fuels toward renewables in the electricity sector, but it also demonstrates repeated, increasingly successful countermobilization by energy incumbents and their allies. The course for Germany's energy transition was largely set with the adoption of a feed‐in tariff law in 1990, but since then the energy transition has been altered by a series of policy‐making episodes, each of which was shaped by the outcomes of the previous episodes; there has been a combination of reinforcing and reactive sequences. This article uses policy windows and advocacy coalition theory, supplemented by work on resistance to carbon pricing, to analyze the four periods in which opponents of the energy transition had the greatest opportunities to limit or reverse it. It makes three main arguments intended to influence future research on energy transitions: (1) episodes of opposition to the feed‐in tariff policy occurred when problem awareness and political commitment converged, (2) the outcomes of those conflicts depended on the balance of mobilization by advocacy and opposing coalitions, and (3) rising household costs due to the renewable energy surcharge drove both problem awareness and the composition of the opposing coalition, which helped lead to a more far‐reaching retrenchment of renewable energy policy in 2014 than in earlier periods.     

Shaping the Scope of Conflict in Scotland’s Fracking Debate: Conflict Management and the Narrative Policy Framework

This study applies a narrative lens to policy actors’ discursive strategies in the Scottish debate over fracking. Based on a sample of 226 newspaper articles (2011–2017) and drawing on key elements of the narrative policy framework (NPF), the research examines how policy coalitions have characterized their supporters, their opponents, and the main regulator (Scottish government). It also explores how actors have sought to expand or contain the scope of conflict to favor their policy objectives. Empirically, only the government strives for conflict containment, whereas both pro‐ and anti‐fracking groups prioritize conflict expansion through characterization contests and the diffusion and concentration of the costs/risks and benefits of fracking. In theoretical terms, the study proposes that Sarah Pralle’s conflict management model, which emphasizes symmetrical strategies of conflict expansion by both coalitions, is a potential tool to revise extant NPF expectations about the different narrative strategies of winning and losing coalitions. Moreover, the fact that policy actors mostly employ negatively rather than positively framed characters in their narratives may be a valid expectation for similar policy conflicts, particularly under conditions of regulatory uncertainty.     

Electric Shock: The 2012 India Blackout and Public Confidence in Politicians

How do disruptions in basic public service delivery shape people’s perceptions of politicians? We offer evidence from the July 2012 blackout in India, the largest in human history. Using data from the India Human Development Survey, we compare confidence in politicians between households that were surveyed during the outage and affected by it (treatment) and only days before it (control). Balance statistics show that the treatment and control groups are statistically indistinguishable, and further tests indicate that there was no disturbance to the pattern of surveying. Far from undermining public confidence in politicians, the outage increased it, as citizens reacted to the anxiety created by the crisis. The implications are normatively troubling, as politicians seem to have reaped benefits from disruptions in public service delivery.     

重楼总皂苷对人胃癌细胞SGC-7901 增殖、迁移和侵袭能力的影响

目的 探讨重楼总皂苷（Rhizoma Paridis total saponin,RPTS)在体外对人胃癌细胞SGC-7901侵袭转移能力的影响。方法 取对数生长期的SGC-7901细胞,用不同浓度RPTS(2.5、5、10、20、40 μg/mL)处理24 h,四甲基偶氮唑蓝（methyl thiazolyl tetrazolium, MTT）法观察RPTS对细胞增殖能力的影响;采用细胞划痕实验、Matrigel transwell小室侵袭实验检测SGC-7901细胞的迁移和侵袭能力。结果 MTT法检测表明RPTS（10、20、40 μg/mL）可明显抑制SGC-7901细胞的增殖能力（P＜0.05）;细胞划痕实验、Matrigel transwell小室侵袭实验结果表明RPTS(2.5、5、10 μg/mL)处理过的SGC-7901细胞,迁移能力和侵袭能力降低（P＜0.05）。结论 RPTS具有抑制人胃癌细胞SGC-7901细胞增殖、迁移和侵袭的能力。     

Factors Driving the Promotion of Hydroelectricity: A Qualitative Comparative Analysis

In the wake of the COP21 conference in Paris, the transition to a low‐carbon energy supply remains a central issue on the political agenda. The deployment of renewable energies is often challenged by multiple issues (e.g., public acceptance, landscape protection, and so forth). Political actors try to overcome such challenges with various measures; however, the policy instruments used vary greatly in their strength. This article questions what factors lead to the adoption of strong policy instruments promoting hydroelectricity. Explanatory factors are derived from Kingdon's multiple streams framework and are analyzed with fuzzy‐set qualitative comparative analysis within the Swiss cantons. The findings show that the strength of policy promoting hydroelectricity depends on the conjunction of mainly two factors: ambitious climate targets and an already well‐established hydroelectricity sector that generates large tax revenues for the cantons. Depending on the context, the strength of left‐wing and green parties as well as the current level of exploitation play an important role with the aforementioned factors.     

Competing Policy Windows in Biotechnology: The FDA,the 21st Century Cures Act,and Laboratory‐Developed Tests

In 2014, the U.S. Food and Drug Administration (FDA) proposed taking a more active role in the regulation of laboratory‐developed tests (LDTs). Meanwhile, the U.S. House of Representatives embarked upon the 21st Century Cures initiative to develop legislation to expedite the development of new biotechnology innovations. During that initiative and in the public comments responding to the draft guidance, there was significant disagreement as to whether the increased FDA regulation of LDTs was beneficial or detrimental to biotechnology. Both the FDA guidance document and the 21st Century Cures Act came about due to the opening of a window of opportunity created by the convergence of circumstances. The question is whether the windows of opportunity are competing and how to resolve this competition. This study will investigate these questions through a qualitative case study. It will also provide recommendations for resolving policy disputes involving wicked problems like biotechnology policy.