微小隐孢子虫感染HCT-8细胞模型的建立及不同阶段虫体形态的观察

利用人回盲肠癌(HCT-8)细胞作为感染细胞,采用不同的接种细胞浓度和培养血清浓度进行培养,观察细胞的生长状况;在培养细胞中加入1×105个经活力检测的微小隐孢子虫卵囊,培养72h后用荧光标记的隐孢子虫卵囊、子孢子染色试剂盒Crypt-a-Glo和Sporo-Glo进行染色,观察不同发育阶段的隐孢子虫形态。结果显示,在六孔培养板中接种10×105个细胞后,24h后长成80%～100%单细胞层,可以用来接种微小隐孢子虫。培养72h后观察到了隐孢子虫各个阶段虫体的形态。结果表明,成功建立了微小隐孢子虫感染HCT-8细胞模型。     

Anspruch der Eigentümergemeinschaft auf Herausgabe der Verwaltungsunterlagen nach Kündigung des WE-Verwalters

Der gekündigte WE-Verwalter ist verpflichtet, nach beendetem Verwaltungsverh?ltnis der Eigentümergemeinschaft die Original-Verwaltungsunterlagen auch für bereits abgeschlossene Abrechnungsperioden herauszugeben. Der Herausgabeanspruch ist – entgegen der E 5 Ob 115/05f: streitiger Rechtsweg – nach nunmehr gefestigter Rsp auf Grund schlüssiger Verweisung des § 52 Abs 1 Z 6 WEG 2002 im wohnrechtlichen Au?erstreitverfahren geltend zu machen.     

《刑法修正案（八）》新增坦白制度的理解与适用

《刑法修正案（八）》第8条规定了坦白从宽情节,这是我国刑事立法上首次确认坦白从宽制度。坦白从宽法定化是落实宽严相济刑事政策,实现司法公正、提高司法效率,走出司法尴尬境地之必然产物。坦白,是指犯罪嫌疑人除构成自首以外的如实供述自己罪行的情形。坦白的主体是＂犯罪嫌疑人＂,可以包括犯罪单位。坦白的成立条件包括：被动归案;如实供述自己的已被司法机关掌握的罪行或者如实供述司法机关虽未掌握、但与已掌握的罪行属同种罪行。坦白从宽是原则,不从宽是例外。避免特别严重后果的发生可以从四个方面进行认定。     

Validation of the Short Amplicon Multiplex Q8 Including the German DNA Database Systems*

Abstract: We have developed a concept to enable the analyzing of degraded stains with limited DNA template quantity. Therefore we have constructed a short tandem repeat (STR) multiplex including the German DNA database systems (Q8). The amplicon lengths are smaller than 280 bp. For the validation of Q8 over 50 degraded samples were investigated. Amplifications were performed with “low copy number” PCR, the number of PCR cycles was increased to 33 and the reaction volume was decreased to 12.5 μL. Compared with the MPX2 and Nonaplex kit, the average success rate was increased using the Q8 kit by approximately 20% and 30%, respectively. The efficiency of a sensitive STR multiplex with reduced amplicon lengths was confirmed in comparing the success rates of Q8 for typing degraded samples and samples with limited amount of DNA template while partial profiles were observed with the majority of the samples using commercially available kits.     

Teilkündigung der Mitbenützung eines Kellerraums zur Aufstellung von Waschmaschinen

Die Benützung einer allen Mietern zug?nglichen Waschküche unterliegt nicht der separaten Aufkündigung nach § 31 MRG, sondern dem § 8 Abs 2 MRG.     

Default entitlements in personal data in the proposed Regulation: Informational self-determination off the table … and back on again?

This paper aims to assess the proposed General Data Protection Regulation through the framework of default entitlements in personal data. The notion of default entitlements comes from economic analysis of law and provides new insights into the implications of the data protection reform. While, under the principle of informational self-determination the default entitlements should lie with the individual, the Commission is shown to assign a great deal of default rights to others, including the Information Industry. This article cautions against the possibility of reducing the European system of data protection rooted in the values of individual autonomy and informational self-determination to a mere set of administrative rules channelling the flow of personal data, yet without a clear direction.     

Characterization of 26 miniSTR loci for improved analysis of degraded DNA samples

An additional 20 novel mini-short tandem repeat (miniSTR) loci have been developed and characterized beyond the six previously developed by our laboratory for a total of 26 non-CODIS miniSTR markers. These new markers produce short PCR products in the target range of 50-150 base pairs (bp) by moving the primer sequences as close as possible-often directly next to the identified repeat region. These candidate loci were initially screened based on their small amplicon sizes and locations on chromosomes currently unoccupied by the 13 CODIS STR loci or at least 50 Mb away from them on the same chromosome. They were sequenced and evaluated across more than 600 samples, and their population statistics were determined. The heterozygosities of the new loci were compared with those of the 13 CODIS loci and all were found to be comparable. Only five of the new loci had lower values than the CODIS loci; however, all of these were much smaller in size. This data suggests that these 26 miniSTR loci will serve as useful complements to the CODIS loci to aid in the forensic analysis of degraded DNA, as well as missing persons work and parentage testing with limited next-of-kin reference samples.     

Keine Unterbrechung eines Unterhaltsvorschussverfahrens durch Konkurs des Schuldners

Die Er?ffnung des Schuldenregulierungsverfahrens über das Verm?gen des Unterhaltsschuldners führt nicht zur Unterbrechung eines Unterhaltsvorschussverfahrens.     

Eigenbedarf – Selbstverschulden

Zwar besteht grunds?tzlich ein Eigenbedarf des Vermieters bei einer 68-m²-Wohnung für einen 5-Personen-Haushalt. Allerdings begründet die Tatsache, dass er nach der Geburt des dritten Kindes 3 Kleinwohnungen neu vermietete, die zusammengelegt eine ausreichende Wohnfl?che von 90 m² bieten würden, den Einwand des selbstverschuldeten Eigenbedarfs, wenn die Zusammenlegung der Kleinwohnungen technisch m?glich, wirtschaftlich sinnvoll und für den Kl finanzierbar gewesen w?re.