干生物基质点微样技术及其在法医毒物学中的应用

干生物基质点(Dried Matrix Spot,DMS)微样技术是通过将微量生物液体(如血液、尿液和唾液等)收集于卡纸上并干燥后形成的样本采集和储存形式.干生物基质点微样技术较传统的采样方法有明显的优势:取样量小,样品处理简单,储存运输方便,目前已应用于流行病学调查、毒药物监测、药代动力学研究和毒代动力学研究等领域....     

响应面分析法优化马兰总黄酮提取工艺研究

目的 应用响应面分析法优化马兰总黄酮的提取工艺。方法 以单因素研究为基础,以总黄酮提取量为响应值,以4因素3水平的响应面分析法优化马兰总黄酮的提取工艺。结果 马兰总黄酮的最优提取工艺条件为：乙醇浓度为40%,液料比为〖DK(〗16.5∶1,〖DK)〗提取时间为2 h,提取次数3次,在该条件下,马兰总黄酮提取量预测值为162.538 mg,实际验证值为156.867 mg,并得出各因素对马兰总黄酮提取量的影响大小依次为乙醇浓度、液料比、提取次数、提取时间。结论 响应面分析法可用于优化马兰总黄酮的提取工艺,实验验证值与模型预测值基本相符。     

论“微时代”网络技术的异化

马克思的"异化"观点揭示了资本主义私有制条件下劳动产品及劳动对劳动者奴役的现象,现代科技"异化"是对马克思"异化"观的引申和发展。现代网络通信技术的发展在给人们生活带来极大便利的同时,又在一定程度上超出了人的控制,甚至成为支配、统治人的异己力量。要消除网络技术的负效应,必须凸显科技的人文关怀,推动网络技术的可持续发展,实现科技与社会的良性互动。     

基于虚拟现实技术的现场勘查训练系统研究

现场勘查是侦查人员必须熟练掌握的基本专业知识和技能,具有很强的实战性和应用性。但是,目前通用的模拟现场勘查训练方法存在一定的局限性。研究开发"基于虚拟现实技术的现场勘查训练系统",借助现代科学技术的最新成果,更新教学理念,创新教学方法,可以克服目前通用的模拟现场勘查训练方法的不足,为现场勘查教学和训练开创一条新的、有效的途径。     

Standardization of Histological Procedures for the Detection of Toxic Substances by Immunohistochemistry in Dipteran Larvae of Forensic Importance

Immunohistochemistry (IHC) technique is an alternative toxicological analysis to detect drugs in insects of forensic importance, but it requires thorough histological procedures. In this study, we tested different fixatives—phosphate‐buffered paraformaldehyde 4% (PP), Carnoy's fluid (CF), Kahle's solution (KS), ethanol in different concentrations, and ethanol associated to PP and CF, time of fixation and histological processes for dipteran larvae's tissue, aiming to develop a sample preparation protocol for IHC application. A suitable fixation was achieved using PP for 12 and 24 h, CF for 3 h, 70% ethanol for 19 days, and 70% ethanol/CF for 2 h/3 h. Postfixation using negative pressure, two immersions in xylene for 30 min each, and one in xylene plus paraffin for 45 min increased tissue preservation. An immunohistochemical test for cocaine detection was performed using monoclonal benzoylecgonine antibody from mouse, peroxidase‐conjugated anti‐mouse IgG and visualized by 3,3′‐diaminobenzidine method showed these histological procedures didn't compromise antigenicity.     

Two cultural worlds in one family

The author discusses the problem of continuity and discontinuity over three generations of a Russian family while describing cultural change in everyday practice during a long historical period. The issue of cultural transmission is investigated because the couples' lineages belonged to conflicting cultural worlds representing two ideologically alien discourses during the Soviet times. While analyzing these two cultural worlds as systems and the conflict between them, the author seeks to answer the question on why both cultural models were rejected by the next generation. What was the character and mechanism of transmission in this case? What are the consequences of cultural conflict for the future of the family itself and for the wider social context in the future cultural discourse in Russia?     

不同培养系统牛体外受精囊胚的质量评价

采用双重荧光分染方法 ,结合免疫外科手术 ,对来自TCM 199和SOF培养系统的牛体外受精囊胚质量进行了评价。结果显示 ,TCM 199培养系统的囊胚发育率为 31.9% (335 / 10 74 ) ,平均细胞总数为 73.1,内胚团细胞数为 16 .8,其中生存细胞占 99.5 % ;SOF培养系统的囊胚发育率为 2 0 .9% (2 4 8/ 1186 ) ,平均细胞总数为 5 6 .2 ,内胚团细胞数为 11.3,其中生存细胞占 96 .5 %。证实 ,使用TCM 199生产的囊胚质量和囊胚发育率优于SOF培养系统。     

高职院校计算机网络课程教学改革探讨

针对当前高职类院校计算机网络课程教学中存在的问题,本文提出了以职业技能为中心制定教学方案的方式,特别指出网络实验室建设和运作好坏是决定计算机网络教学成败的关键,并给出具体分类及基本建设方案。     

医疗技术发展风险抗辩

本文从医疗纠纷处理的难点出发 ,结合医疗损害事件向无过错责任转化的趋势。提出医疗核技术发展风险抗辩 ,作为医方免责理由 ,并分析了此抗辩的构成要件 ,与相关因素。     

长角血蜱的免疫研究进展

以长角血蜱疫苗候选抗原为例,从长角血蜱蛋白水解酶、蜱生殖和发育相关分子及RNA干扰技术、IgG结合蛋白技术几方面概述了近年来国内外有关蜱疫苗的研究现状,并展望了蜱疫苗的发展趋势。