微小牛蜱幼蜱cDNA表达文库的构建

为了研究微小牛蜱功能性抗原基因,从微小牛蜱(Boophilus microplus)幼蜱中提取总RNA并分离纯化mRNA。采用Oligo(dT)引物合成双链cDNA,在其两端加EcoRⅠ、HindⅢ定向接头,用Mini Column Fractionation凝胶过滤柱纯化后定向克隆到λSCREEN载体。经体外包装,成功构建了我国微小牛蜱幼蜱cDNA表达文库,铺板测定原始库容量为4.5×105PFU,扩增后的滴度为7.2×1010PFU/mL。     

损伤伴心血管系统疾病死亡42例法医病理学研究

目的探讨心血管系统疾病伴外伤死亡案例法医病理学特点,并从伤病的角度揭示两者之间的联系。方法42例心血管系统疾病伴损伤死亡案例均接受系统病理学解剖和组织学观察,且死因明确。结果42例中40岁以上者占到64.28%,男性较女性死者多。擦伤和挫伤在损伤中比例最大共占到80.95%,冠心病和高血压病死亡者各占45.24%、16.67%。损伤和疾病共存死亡案例共20例。结论在死因分析中年龄、性别、损伤类型、疾病分布有其规律性,死者中年龄偏老年化,损伤多为钝器、摔跌所致擦伤、挫伤。疾病中冠心病、高血压为最多。损伤和疾病对死因共同起作用的案例主。     

A Case of Sudden Infant Death Due to Incomplete Kawasaki Disease

Although Kawasaki disease (KD) is a self‐limiting disease, it may cause sudden cardiac death. Diagnosis of KD is principally based on clinical signs; however, some infant cases do not meet the criteria. Such cases are identified as incomplete KD. The sudden death risk in incomplete KD cases is similar to conventional KD. In our 5‐month‐old case, he had been admitted to a hospital for a fever and suppuration at the site of Bacille de Calmette et Guerin (BCG) vaccination. However, after discharge from the hospital, his C‐reactive protein (CRP) levels declined, he got indisposed and died suddenly. A medico‐legal autopsy revealed myocarditis, coronaritis, platelet‐aggregated emboli in coronary arteries, and myocardial degeneration, suggesting that the fatal myocardial infarction was due to thrombus emboli in the coronary arteries. Forensic pathologists therefore should pay attention to the cardiac pathology originated from incomplete KD as a potential cause in cases of sudden infant death.     

Commode Cardia—Death by Valsalva Maneuver: A Case Series

The Valsalva maneuver is used in clinical medicine for the diagnosis and/or treatment of various cardiovascular conditions. It can also be used in activities of daily living, such as defecation. Due to the cardiovascular effects produced during the Valsalva maneuver, it may be contraindicated in certain medical conditions and could be a trigger of sudden cardiac death. The incidence and prevalence of death following Valsalva maneuver in the presence of underlying cardiovascular disease, or “commode cardia,” has not been examined. In 2012, the Wayne County Medical Examiner's Office (Detroit, MI) investigated 21 deaths that occurred on the toilet, fourteen of which were due to cardiovascular disease. In another 31 deaths in the bathroom due to cardiovascular disease, the possibility that the decedent defecated immediately prior to death could not be excluded. Hence, the incidence of commode cardia in this population ranges from 2.3 to 7.4% of all cardiovascular‐related deaths.     

When benefitting a patient increases the risk for harm for third persons — The case of treating pedophilic Parkinsonian patients with deep brain stimulation

This paper investigates the question whether it is ethically justified to treat Parkinsonian patients with known or suspected pedophilia with deep brain stimulation — given increasing evidence that this treatment might cause impulse control disorders, disinhibition, and hypersexuality. This specific question is not as exotic as it looks at a first glance. First, the same issue is raised for all other types of sexual orientation or behavior which imply a high risk for harming other persons, e.g. sexual sadism. Second, there are also several (psychotropic) drugs as well as legal and illegal leisure drugs which bear severe risks for other persons. We show that Beauchamp and Childress' biomedical ethics fails to derive a veto against medical interventions which produce risks for third persons by making the patients dangerous to others. Therefore, our case discussion reveals a blind spot of the ethics of principles. Although the first intuition might be to forbid the application of deep brain stimulation to pedophilic patients, we argue against such a simple way out, since in some patients the reduction of dopaminergic drugs allowed by deep brain stimulation of the nucleus subthalamicus improves impulsive control disorders, including hypersexuality. Therefore, we propose a strategy consisting of three steps: (1) risk assessment, (2) shared decision-making, and (3) risk management and safeguards.     

Glamorizing sick bodies: how commercial advertising has changed the representation of HIV/AIDS

As a consequence of pharmaceutical advancements, HIV is no longer described in terms of the absence of health or presence of illness, and advertisements promoting anti-AIDS medications commercialize idealized and desirable bodies. The present study discusses representations of HIV/AIDS in commercial advertising and their change over time. The article traces the shift in AIDS/HIV representations in commercial advertising from the early 1990s, when images of decay and disease represented AIDS, to nowadays, when the wider availability of antiretroviral medications and their ability to prolong life produced new representations of HIV-afflicted bodies. Claiming that HIV individuals can lead a normal life where everything is possible, advertising has re-established the definition of a sick body. On the other hand, this marketing approach has important social implications because such representations minimize the seriousness of HIV infection and fail to take into account the real dangers of contracting HIV and to accurately represent the life with HIV and AIDS.     

Sudden Unexpected Death Due to Graves' Disease During Physical Altercation

We report a case of a 30‐year‐old woman who suddenly collapsed after having a physical altercation with her husband. Despite immediate resuscitation, she died on arrival at the hospital. The victim's parents requested an autopsy because they believed that their daughter was killed by her husband. Postmortem examination revealed that the victim had a diffusely enlarged thyroid gland and cardiomegaly with left ventricular hypertrophy. There was no evidence of significant trauma on the body. Further postmortem thyroid function tests and review of her medical history indicated that her death was due to Graves' disease. To the best of our knowledge, this is the first case reported of sudden death due to cardiac arrhythmia from Graves' disease induced by physical and emotional stress associated with the criminal activity of another person. The autopsy findings are described. In addition, the literature is reviewed and the significance of postmortem evaluation of thyroid hormones in the cases of sudden death is discussed.     

Carbon Monoxide Poisoning and Death in a Large Enclosed Ventilated Area

A 55‐year‐old man with a medical history of tobacco use suddenly collapsed while power washing an empty indoor pool in a hotel. The decedent was transported to the local hospital where he was pronounced. A postmortem examination revealed atherosclerotic heart disease and bilateral pulmonary edema and congestion. A postmortem blood carbon monoxide (CO) level was 27% saturation, and a CO performed on hospital admission blood was 49% saturation. CO poisoning is a common cause of toxicological morbidity and mortality in the United States. The circumstances most often occur in an enclosed environment and may be intentional or unintentional. CO poisoning has been reported in open, well‐ventilated spaces, but rarely results in death. A warning label was present on the engine clearly stating the dangers of CO emission. However, there was a false sense of security due to the large size of the pool room and the presence of industrial blowers that were being used for ventilation.     

人体不同体液内PSA含量及其法医学意义

目的检测人体不同体液内前列腺特异抗原(PSA)含量,探讨其法医学价值。方法收集成年人(19～63岁)晨尿40份(男28份、女12份)、血液58份(男45份、女13份)、唾液25份(男14份、女11份);青少年(10～15岁)男性晨尿205份;哺乳期(25～31岁)女性乳汁9份;使用Cobas e411型全自动电化学发光免疫分析系统及T-PSA定量测定试剂盒,检测各样本T-PSA含量;分析不同体液及不同年龄青少年男性尿液PSA含量差异。结果除男、女性唾液外,其它样本均可检测到PSA,其中成年男性尿液含量最高,与其它体液比较具有显著性差异(P<0.000 1);青少年男性各年龄组尿液PSA含量随年龄逐年增高,11岁及以下年龄组含量不足1ng/mL,14岁及以上年龄组可超过1 000ng/mL。结论前列腺发育成熟的男性尿液PSA含量较高,在进行精液斑的法医学检验时应给予充分注意。     

GFP标记的重组新城疫病毒HN基因乳酸菌表达载体的构建及原核表达

为构建具有示踪特性的新城疫病毒(NDV)HN基因乳酸菌穿梭表达载体,采用PCR方法扩增NDV HN基因与绿色荧光蛋白(GFP)基因,将两基因克隆至乳酸菌表达载体pW425et中,转化入表达宿主菌BL21(DE3)后利用IPTG诱导表达。用荧光显微镜检查重组菌的绿色荧光效应,采用SDS-PAGE和Western-blotting检测其在大肠杆菌中的表达情况。结果显示,NDV HN和GFP基因与表达载体pW425et正确重组。荧光显微镜下可见阳性重组菌落的绿色荧光,且该重组菌传代30代次以上,荧光效应依然稳定。SDS-PAGE显示表达出约63ku的条带,与已知表达的HN蛋白大小相符。Western-blotting进一步证实该蛋白能被NDV阳性血清所识别,具有反应原性。构建了GFP标记的重组NDV HN基因乳酸菌穿梭表达载体且能在大肠杆菌中表达。