Keine Unterbrechung eines Unterhaltsvorschussverfahrens durch Konkurs des Schuldners

Die Er?ffnung des Schuldenregulierungsverfahrens über das Verm?gen des Unterhaltsschuldners führt nicht zur Unterbrechung eines Unterhaltsvorschussverfahrens.     

H5N1亚型禽流感病毒NS1基因的克隆及表达

利用RT-PCR扩增了2株H5N1亚型禽流感病毒NS1基因,并将其克隆到pMD 18-T载体上,进行序列分析。结果显示,这2株禽流感病毒NS1基因核苷酸序列的同源性为70.2%,分别属于NS等位基因群A和等位基因群B。再将克隆的NS1基因插入到pET-28a质粒中构建原核表达载体,将其转化到DH5α大肠埃希氏菌感受态细胞中,经双酶切鉴定及序列分析,表明获得了重组质粒pET-52NS1和pET-174NS1。经SDS-PAGE分析,重组质粒转化BL21(DE3)(pLysS)感受态细胞后,经IPTG诱导,获得了分子质量约为30 ku的NS1融合蛋白。用AIV多克隆血清做Western-blotting分析,发现来自2个等位基因群的NS1蛋白都具有较好的抗原活性。     

Lutheran血型系统的分子遗传学基础

Lutheran血型系统含有18个抗原,LU1/LU2、LU18/LU19抗原位于LU/B-CAM糖蛋白上。LU/B-CAM糖蛋白的蛋白质部分由LU基因表达,LU基因位于19q13.2-13.3,含15个外显子、14个内含子。LU基因在转录时发生变位剪接,产生2.5、4.0kb前体mRNA。LU基因在镰状红细胞、卵巢癌和结肠上皮细胞癌呈高表达。LU/B-CAM糖蛋白是层粘素特异性受体。     

人体基因财产权研究——“人格性财产权”的证成与施用

按照经典权利理论中人/物、人格权/财产权的二元界分逻辑,人体基因作为整全的人身的组成部分,处于人格权的保护之下,被禁止用于谋利性交易。然而,随着基因科技的发展及基因的产业化应用,人/物的界限被内移到了人自身,人体基因也日益外化而具有独立的经济价值。当基因科技的施为力量已在某种程度上消弭了经典权利理论所依仗的人/物二元界分的基础时,试图非此即彼地以人格权或财产权来界定人体基因的法律属性,都不会实现关于基因利益的公平分配和确保人格不被减等的结果。问题的出路,似乎在于在人格权和财产权的混同处,提出一个新的人格性财产权的范畴界定人体基因的法律属性,并综合运用人格权和财产权的保护机制来保障基因资源提供者的利益。     

Race-Based Peremptory Challenges: An Empirical Analysis of Litigation from the U.S. Court of Appeals, 2002–2006

This paper examines race-based peremptory challenges. Such challenges occur during the voir dire jury selection process. The process allows both the defense and the prosecution to strike jurors who they believe will not decide cases fairly. However, in the case of Batson v. Kentucky 476 U.S. 79 (1986), the Supreme Court ruled that race could not be used as a factor in eliminating prospective jurors. This paper examines federal litigation for five years in which it was alleged that race was used as a factor in removing a juror. An examination of the cases revealed that most of the cases involved sole male litigants who allege that there were multiple race-based peremptory challenges used in their cases. Moreover, most of the cases that led to the allegations involved violent offenses. Other case characteristics are noted, but of most significance was the finding that most appellants lost their cases. As such, the courts felt that most of the challenges were, in fact, race neutral. The implications of this research are discussed. This study was funded by an undergraduate research grant from Penn State University.     

PCR-DGGE法检测浮游生物16S rDNA在溺死鉴定中的应用

目的建立并评估PCR-DGGE法检测浮游生物对溺死鉴定的应用价值。方法大白兔30只随机分为3组:溺死组(n=12),死后抛尸组(n=12)和对照组(n=6);溺死组和死后抛尸组又分为2个亚组:东湖水域组(n=6)和墨水湖水域组(n=6)。死后提取心血和肺、肝、肾、脑等组织,匀浆后,采用Percoll密度梯度离心法分离浮游生物并提取其DNA,PCR扩增浮游生物特异的16S rDNA片段后分别用琼脂糖凝胶电泳及DGGE检测分析。2个溺死案例检材同法检验。结果溺死组各组织器官中浮游生物检测多呈阳性:肺(100%)、肝(83%)、肾(75%)、心血(83%)、脑(42%);死后抛尸组仅2例肺组织(16.7%)检出阳性;对照组全部阴性。溺尸肺组织DGGE分型图谱与相应溺死点水样分型图谱相似,而与非溺死点水样分型结果差异显著。2实际案例均呈阳性。结论本方法不仅有助于定性诊断溺死,而且通过比较产物的多样性可以推断溺死地点,在法医学溺死鉴定中具有较大实用价值。     

Two examples of null alleles at the D19S433 locus due to the same 4 bp deletion in the presumptive primer binding site of the AmpFlSTR Identifiler kit

Two cases of allelic loss at the D19S433 locus after multiplex PCR with the AmpFlSTR Identifiler kit (Applied Biosystems) are described. In both cases the failure of PCR resulted in genetic inconsistencies due to opposite homozygosity. After singleplex PCR with published primers additional alleles were observed and Mendelian inheritance was restored. These PCR products were sequenced and in both cases the same 4 bp deletion near the 3′ end of the repeat region was detected in two alleles of different length. The frequency of these null alleles (two events in 1026 allelic transfers) amounts to 0.0019 (95% confidence limits: 0.0002-0.0070).     

以“三创一办”为平台 努力践行“贵阳精神”

"贵阳精神"作为贵阳的城市灵魂和发展动力,必须在推进城市发展的实践中才能充分体现其核心价值。目前正在广泛开展的"三创一办"为践行"贵阳精神"提供了一个难得的机遇和平台。