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211.
Carolyn A. Lewis B.S. Tiffany R. Layne M.S. Sarah J. Seashols‐Williams Ph.D. 《Journal of forensic sciences》2019,64(6):1823-1830
Molecular‐based approaches for biological source identification are of great interest in the forensic community because of a lack of sensitivity and specificity in current methods. MicroRNAs (miRNAs) have been considered due to their robust nature and tissue specificity; however, analysis requires a separate RNA extraction, requiring an additional step in the forensic analysis workflow. The purpose of this study was to evaluate miRNA detection in blood, semen, and saliva using DNA extraction methods commonly utilized for forensic casework. RT‐qPCR analysis revealed that the tested miRNAs were consistently detectable across most tested DNA extraction methods, but detection was significantly reduced compared to RNA extracts in some biological fluids. DNase treatment was not necessary to achieve miRNA‐specific results. A previously developed miRNA panel for forensic body fluid identification was evaluated using DNA extracts, and largely demonstrated concordance with results from samples deriving from RNA extracts of semen, blood, and saliva. 相似文献
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《国际公共行政管理杂志》2013,36(9-10):835-848
Abstract The new concept of social responsibility is strongly linked to the idea of the social enterprise as a living system, that is, a system capable of regenerating itself by producing “knowledge” and “trust” resources. Nevertheless, these resources can only spring from a tight relationship with the reference environment in which the enterprise operates. This paper aims at making some first considerations on the relationship between social responsibility and the environment, and upon how the environment may develop in symbiosis with the “living company.” 相似文献
216.
Cheng Xu M.D. Lei Feng Ph.D. Fan Yang M.D. Jing Jia Ph.D. An‐Quan Ji M.D. Lan Hu Ph.D. Cai‐Xia Li Ph.D. 《Journal of forensic sciences》2015,60(3):783-786
In forensic genetic analyses, mixtures of various biological materials are common samples. Micromanipulation, which is performed based on differences in cellular morphology, is an effective method for the isolation of cells from mixtures. In this study, mucosal cell was isolated from somatic cellular mixtures (blood and saliva) based on micromanipulation and a low volume‐PCR (LV‐PCR) platform. One hundred and twenty‐six parallel LV‐PCR processes were performed using an Identifiler® kit, with 107 reactions yielding single‐source DNA profiles. Among them, 54 full profiles (50%) and 37 partial profiles (13–15 loci) were obtained. Based on the above method, we obtained a single‐source DNA profile from a cigarette butt contaminated by two victims’ blood in a murder case. The generated genotype was used to query a DNA database, and a perfect match was found. 相似文献
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Kathleen A. Hauther B.A. Kelly L. Cobaugh M.S. Lee Meadows Jantz Ph.D. Tim E. Sparer Ph.D. Jennifer M. DeBruyn Ph.D. 《Journal of forensic sciences》2015,60(5):1234-1240
Postmortem succession of human‐associated microbial communities (“human microbiome”) has been suggested as a possible method for estimating postmortem interval (PMI) for forensic analyses. Here we evaluate human gut bacterial populations to determine quantifiable, time‐dependent changes postmortem. Gut microflora were repeatedly sampled from the proximal large intestine of 12 deceased human individuals as they decayed under environmental conditions. Three intestinal bacterial genera were quantified by quantitative PCR (qPCR) using group‐specific primers targeting 16S rRNA genes. Bacteroides and Lactobacillus relative abundances declined exponentially with increasing PMI at rates of Nt = 0.977e?0.0144t (r2 = 0.537, p < 0.001) and Nt = 0.019e?0.0087t (r2 = 0.396, p < 0.001), respectively, where Nt is relative abundance at time (t) in cumulative degree hours. Bifidobacterium relative abundances did not change significantly: Nt = 0.003e?0.002t (r2 = 0.033, p = 0.284). Therefore, Bacteroides and Lactobacillus abundances could be used as quantitative indicators of PMI. 相似文献
218.
DNA profiles were generated via direct amplification from blood and saliva samples deposited on various types of swab substrates. Each of the six non-FTA substrates used in this research was punched with a Harris 1.2 mm puncher. After 0.1 μL of blood or 0.5 μL saliva, samples were deposited on each of these punches, samples were pretreated with one of four buffers and washing reagents. Amplification was performed using direct and nondirect autosomal and Y-STR kits. Autosomal and Y-STR profiles were successfully generated from most of these substrates when pretreated with buffer or washing reagents. Concordant profiles were obtained within and between the six substrates, the six amplification kits, and all four reagents. The direct amplification of substrates which do not contain lysing agent would be beneficial to the forensic community as the procedure can be used on evidence samples commonly found at crime scenes. 相似文献
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