浙江汉族人群6个STR基因座的遗传多态性的调查

目的进一步完善浙江省汉族人群STR基因座遗传多态性的调查,为其应用提供基础数据。方法采用AmpF1STRSGMplus和AmpF1STRCoficer反应试剂盒,使用ABI310型基因分析仪对浙江汉族人群200名无关个体血样进行了D16S539、D2S1338、D19S433、TH01、TPOX和CSF1PO6个STR基因座遗传学分析。结果分别发现了9、15、15、11、8、10个等位基因,发现的基因型分别为23、42、35、19、16、17个,其分布经X2检验均符合Hardy-Weinberg平衡定律,并分别统计了6个STR基因座的H、DP、PM、PE及PIC参数。结论6个STR基因座适合法医学应用。     

PCR法对HLA-DQ_α基因的分型及其在性犯罪鉴定中的应用

本文应用 PCR 法及 ASO 探针斑点杂交技术,对100例无关个体血液 DNA 及10例性犯罪案件混合斑中精子 DNA 进行了 HLA-DQα基因的扩增及其 DNA 分型。结果正常人0.1～0.3μgDNA 就能满足 DQα基因扩增的需要。在100例个体中可以观察到由4种等位基因组成的10种 DQα基因型。10例不同条件的混合斑中精子 DNA 经30～60次扩增后与 ASO 探针杂交均能准确地判定 DQα基因型。HLA-DQα是个体识别能力较强的遗传标志。本文为性犯罪中精子来源的个体识别提供了一个新方法,具有一定的实用价值。     

WTO框架下云南农业结构的调整和优化

在我国加入WTO和普通农产品过剩的前提下,调整和优化我省农业结构,必须提高产品质量和增加具有竞争力强的品种,必须将产业链进行必要的延伸,必须改变经营者的状态。     

不同分型方法的STR分型差异

目的调查不同的STR分型系统之间分型的一致性。方法 10 0例不同个体的DNA样本分别用单位点聚丙烯酰胺凝胶银染法和PowerPlex16System试剂盒对 13个法医学常用STR位点进行基因分型 ,并比较两种不同分型系统间的分型结果。结果 1例样本在D8S1179位点出现了分型不一致的结果 :银染法的基因型为 12 / 14 ,而用PowerPlex16System试剂盒的分型则为 12 / 15。结论不同的STR分型系统可导致不同的基因分型     

体外DNA扩增技术鉴定血痕性别三例报告

本文报道用体外DNA扩增技术对3例刑事案中的人血痕标本进行性别鉴定。其方法是从血痕标本中微量抽提DNA,用蛋白酶K进行消化。然后用两组引物Y1.1与Y1.2和Alu9.1与Alu9.2及国产FD耐热DNA聚合酶进行聚合酶链反(PCR)扩增DNA,电泳分析Y及Alu重复序列,从而判断血痕的性别。     

Dynamics of Trade in Value-Added in “Factory Asia”

ABSTRACT

Trade patterns in East Asia are termed the “Factory Asia” model, whereby Asia functions as a “global factory” that imports intermediate goods from its regional networks and then assembles and exports them as finished goods to higher-income developed countries. In 2001, China’s accession into the World Trade Organisation consolidated this pattern by becoming the core economy in this model. However, is this pattern still valid after more than a decade of rapid development in East Asian countries? The main objective of this article is to examine the evolution of this pattern of trade in East Asian countries. Although the key findings of this study show that the Factory Asia model continues, it is changing as different East Asian countries capture more value in global value chains. The gaps in the rate of upgrading are identified and mainly attributed to differences in government policies and competition. However, the dependence on foreign inputs still remains an important part of high-technology production in East Asian countries. Hence, the idea that East Asia is evolving from a “factory” into a “Research & Development hub” remains far-fetched.     

典型引领价值功能研究的回顾与思考

在一个文化多元、媒体多样的时代里，如何打破单向性灌输模式，多维、互动地发挥典型引领的价值功能成为一个重大问题。基于文化强国的使命感，基于核心价值如何具有巨大而持续的情感能量、如何实现符号内化等基本问题，通过总结、反思中国典型引领中的价值功能，引入互动仪式链视角，寻求完善社会主义核心价值的建构机制以及社会认同机制。     

刑事印证初论

刑事印证是我国刑事诉讼的主要证明方式,它具有层次性;刑事印证的表现形式为证据链,核心为证据联结点;证据联结点的差异有合理的差异和非合理差异、重要的差异和非重要的差异之分,证据间的一致有实质一致和虚假一致之别;对既具有间接证据也有直接证据的一般刑事案件,要求达到全案证据链的完整性即可,对全部据间接证据定案的案件,则要达到证据链的闭合性.     

连锁加盟式合同诈骗的犯罪形式及侦查策略

连锁加盟式合同诈骗虽不是犯罪分子非法敛财的新手段,但是随着近一段时间"炒房热"的逐步退温,民间资本逐渐转向投资连锁加盟店等更为理性的项目。犯罪分子打着连锁加盟的旗号,对手中有闲余资金的群众大肆进行诈骗,犯罪手法更加隐蔽,社会危害性更大。本文拟从连锁加盟式合同诈骗的犯罪手法展开分析,对此类案件的侦查、打击进行研究。     

DNA Analysis of Natural Fiber Rope*

Abstract: When rope is found at a crime scene, the type of fiber is currently identified through its microscopic characteristics. However, these characteristics may not always unambiguously distinguish some types of rope from others. If rope samples contain cells from the plants of origin, then DNA analysis may prove to be a better way to identify the type of rope obtained from a crime scene. The objective of this project was to develop techniques of DNA analysis that can be used to differentiate between ropes made from Cannabis sativa L. (hemp), Agave sisalana Perrine (sisal), Musa textilis Née (abaca, “Manila hemp”), Linum usitatissimum L. (flax), and Corchorus olitorus L. (jute). The procedures included extracting the DNA from the rope, performing polymerase chain reaction (PCR) using the extracted DNA as a template, and analyzing the DNA products. A primer pair for PCR, chosen from within a chloroplast gene for the large subunit of ribulose bisphosphate carboxylase/oxygenase, was designed to be specific for plant DNA and complementary to the genes from all five plants. The resulting PCR fragments were approximately 771 base pairs long. The PCR fragments, distinguished through base sequence analysis or restriction enzyme analysis, could be used to identify the five different rope types. The procedure provides a useful addition to visual methods of comparing rope samples.