公共服务供给机制转型中的公益性岗位研究

我国自2002年起开始施行公益性岗位的政策,这一政策对拓宽就业渠道、缓解失业压力起到了一定的作用。但目前依靠政府进一步开发和购买公益性岗位的难度很大。本文对国内外公共服务供给机制转型进行了比较分析,在此基础上提出以下建议:我国应进一步完善公共服务的供给机制,以社区为平台,通过推动购买服务的方式,调动社会组织服务社区和吸收就业的功能,以推动公益性岗位的增加。     

“两个普遍”要求乡镇（街道）工会建设新突破

当前,乡镇(街道)工会已成为工会组织体系中承上启下的重要环节,加强乡镇(街道)工会建设,落实"组织起来、切实维权"工作方针,亟待解决"有人干事"和"有钱办事"等问题,按照工会规范化建设要求,明确乡镇(街道)总工会法律地位,增强基层工会活力,确保依法推动企业普遍建立工会组织,依法推动企业普遍开展工资集体协商,即"两个普遍"工作目标的实现。     

关于转型期企业共青团建设的几点思考

企业团组织引导青年发展,应基于青年特点和企业的实际展开。企业团组织要发挥政治上引领青年、帮助青年成才、对青年的考核等方面的作用。企业团建工作要从健全组织机构、介入企业文化、推进组织创新、规范组织生活、开展青年养成教育等方面入手。     

Digital Wiretap Warrant: Improving the security of ETSI Lawful Interception

Lawful Interception (LI) of data communications is an essential tool for Law Enforcement Agencies (LEA) in order to investigate criminal activities carried out or coordinated by means of Internet. However, the ability to secretly monitor the activities of citizens also has a great impact on civil rights. Therefore, democratic societies must prevent abuse and ensure that LI is only employed in specific cases with justifiable grounds or a probable cause. Nowadays, in many countries each interception must be authorized by a wiretap warrant, usually issued by a judge. However, this wiretap warrant is merely an administrative document that should be checked by the network or service operator before enabling the monitoring of its customers, whose communications are later handed over to a LEA in plaintext. This paper proposes the idea of employing a Digital Wiretap Warrant (DWW), which further protects the civil liberties, security and privacy of LI by ensuring that monitoring devices can only be enabled with a valid DWW, and by encrypting the captured data so only the authorized LEA is able to decrypt those communications. Moreover, in the proposed DWW framework all digital evidence is securely time-stamped and signed, thus guaranteeing that it has not been tampered with, and that a proper chain of custody has been met. In particular this paper proposes how to apply the DWW concept to the lawful interception framework defined by the ETSI LI Technical Committee, and evaluates how the additional security mechanisms could impact the performance and storage costs of a LI platform.     

当前国际投资促进机构运行的特征与走势

后金融危机时代,各国为从金融危机中尽快复苏经济普遍加强了投资促进工作,国际投资促进机构运行呈现新特征。通过深入研究新特征,密切关注其走势及采取的积极应对措施。为进一步理顺我国投资促进体制机制、创新投资促进方式、推动扩大内需、提升投资服务质量、提高投资促进效益提供启示,深化我国投资促进工作,提升国际竞争力。     

The Management of Democratic Elections in Africa (MDEA) programme at the university of South Africa: Is this the right path towards African solutions?

There have been a number of initiatives in Africa to rid the Continent of political instability, conflict, poverty and disease. Many have argued that aid from the West has helped Africa in many ways—from stopping wars, to food relief and rescuing the African environment. Yet others have slated Western involvement which they contend, frequently leaves Africans dependant rather than capable of solving their own problems. In line with the pan-Africanist and the African Union (AU) ideals, there is now a realisation by Africans that there is a need to find African solutions to African challenges and problems. This review article explores the University of South Africa’s (Unisa’s) Management of Democratic Elections in Africa (MDEA) programme, by looking at its objectives and the reason why it must be labelled a Programme, its mandate, which is in line with the brief of the AU and pan-Africanist ideals. The article focuses on how Unisa’s programme responds to the African Charter on Democracy, Elections and Governance (ACDEG); and how it has attempted to facilitate knowledge for peace in Africa, through empowering electoral officials from various African states. The article concludes by looking at the successes and limitations of the Programme over a five-year period, from its inception in 2011.     

广东省猪源葡萄球菌耐药性分析及多重耐药基因cfr的流行现状

为了解2013-2017年间广东省猪源葡萄球菌的耐药情况及多重耐药基因cfr的流行现状,采集生猪养殖场和交易市场猪鼻腔拭子分离葡萄球菌,采用琼脂稀释法测定分离菌株对14种抗菌药物的敏感性,并用PCR检测mecA和cfr基因的携带情况。结果显示,在采集的4099份样品中分离到葡萄球菌1485株,其中金黄色葡萄球菌173株。猪源葡萄球菌对红霉素、四环素、泰妙菌素、克林霉素、沃尼妙林和氟苯尼考耐药严重,耐药率分别为91.0%、90.2%、87.7%、84.6%、84.6%及82.0%,对利福平和利奈唑胺相对敏感,未发现万古霉素耐药菌株;庆大霉素、四环素、红霉素和环丙沙星的耐药率呈现逐年上升的趋势。在1485株葡萄球菌中,mecA和cfr的检出率分别为39.9%和12.3%。cfr阳性菌株常携带多种耐药基因,cfr基因的遗传背景也呈现多样化。结果表明,广东省猪源葡萄球菌的耐药情况严重,cfr的检出率高,应加强养殖业中抗菌药物的规范和合理使用,减缓耐药性的产生和传播。     

口蹄疫病毒O型和A型交互反应性单克隆抗体的筛选与鉴定

不同血清型口蹄疫病毒(FMDV)之间不能产生交叉免疫保护,但是血清学的交叉反应确实存在。本研究旨在建立一种筛选型间交互反应性抗体的方法,以期为解析FMDV血清型间保守的抗原结构奠定基础。本方法主要依据单个B细胞抗体技术,首先采用两种不同的荧光染料FluoProbes 647H和Pacific Blue分别标记O型与A型FMDV 146S灭活抗原,然后利用流式细胞分选技术,从免疫牛外周血单个核细胞(PBMCs)中分选出结合两种抗原的特异性B细胞,通过单个B细胞抗体基因测定,获得Ig G抗体重链与轻链可变区编码序列,并将可变区基因插入含有牛IgG抗体恒定区的pcDNA3.4载体中,构建IgG抗体的重链与轻链表达质粒。将重链与轻链表达质粒共转染中国仓鼠卵巢悬浮细胞(CHO-S)进行完整抗体表达。结果显示,通过该方法获得了5株FMDV特异性单克隆抗体,其中4株(H64、R82、I16、R29)经间接免疫荧光(IFA)检测都可特异性识别O和A型FMDV;ELISA结果显示,H64、R82、I16与O、A型抗原均具有较好的亲合力;Western-blot结果显示,I16可特异性结合O、A型FMDV结构蛋白VP2中的线性表位,说明在衣壳蛋白VP2上存在血清型间保守的抗原表位,通过本研究进一步加深了对FMDV抗原结构的认识。     

牛传染性鼻气管炎病毒SYBR GreenⅠ实时荧光定量PCR检测方法的建立

为了检测牛传染性鼻气管炎病毒,本研究建立了牛传染性鼻气管炎病毒SYBR Green I实时荧光定量PCR检测方法。通过设计特异性引物扩增病毒基因,将扩增的目的片段(119 bp)连接到pMD19-T载体中,构建重组质粒。将重组质粒作为阳性标准品,用于SYBR Green I实时荧光定量PCR检测方法的建立。结果显示,特异性引物扩增的目的片段约为119 bp,符合预期的大小,经测序鉴定所扩增的目的片段正确。敏感性结果显示,用该方法检测阳性质粒标准品的最低限度为3.04 X101 copies/mL,是Nano-PCR的10倍。将牛病毒性腹泻病毒、牛呼吸道合胞体病毒、牛副流感病毒3型与牛传染性鼻气管炎病毒进行特异性检测。结果显示,只有牛传染性鼻气管炎病毒被检测到,其他病毒均未有特异性的扩增曲线,表明该方法的特异性良好。批内和批间重复变异系数均小于1%,显示该方法具有良好的重复性。用建立的方法对20份疑似IBRV的临床样品进行检测,并与Nano-PCR检测方法进行对比,结果显示本方法的阳性样晶率高于Nano-PCR检测方法。本研究建立的牛传染性鼻气管炎病毒SYBR Green I实时荧光定量PCR方法特异性好、灵敏度高、重复性好,可用于牛传染性鼻气管炎病毒的检测。     

适合鸭瘟病毒弱毒增殖的MHC单倍型鸭胚肝间充质干细胞的建立

为了建立适于鸭瘟病毒(DPV)弱毒增殖的细胞系,利用4个主要组织相容性复合体(MHC)单倍型鸭品系,体外分离鸭胚肝细胞,在干细胞因子、白细胞抑制因子和成纤维细胞生长因子诱导下,获得鸭胚肝间充质干细胞(DuLMSC)。接种DPV疫苗株C-KCE后,结果显示:F1~F10代均能稳定出现CPE;将F10病毒接种细胞24 h后,通过扫描电镜在胞质、胞核及细胞间隙均能够观察到典型的疱疹病毒粒子;F1至F10代均能扩增出DPV UL2基因片段,序列测定结果表明F1、F5、F10代的扩增产物与NCBI中公布的DPV株(EU082088)的核苷酸同源性为100%;组织培养半数感染量和一步生长曲线结果表明,源自B4系单倍型鸭的DuLMSC细胞系增殖DPV的病毒含量明显高于B1、B2和B3系。本研究为稳定培养鸭瘟病毒提供了优良稳定的实验材料。