Assessing a novel room temperature DNA storage medium for forensic biological samples

The ability to properly collect, analyze and preserve biological stains is important to preserving the integrity of forensic evidence. Stabilization of intact biological evidence in cells and the DNA extracts from them is particularly important since testing is generally not performed immediately following collection. Furthermore, retesting of stored DNA samples may be needed in casework for replicate testing, confirmation of results, and to accommodate future testing with new technologies.A novel room temperature DNA storage medium, SampleMatrix™ (SM; Biomatrica, Inc., San Diego, CA), was evaluated for stabilizing and protecting samples. Human genomic DNA samples at varying amounts (0.0625-200 ng) were stored dry in SM for 1 day to 1 year under varying conditions that included a typical ambient laboratory environment and also through successive freeze-thaw cycles (3 cycles). In addition, spiking of 1-4× SM into samples prior to analysis was performed to determine any inhibitory effects of SM. Quantification of recovered DNA following storage was determined by quantitative PCR or by agarose gel electrophoresis, and evaluation of quantitative peak height results from multiplex short tandem repeat (STR) analyses were performed to assess the efficacy of SM for preserving DNA.Results indicate no substantial differences between the quality of samples stored frozen in liquid and those samples maintained dry at ambient temperatures protected in SM. For long-term storage and the storage of low concentration samples, SM provided a significant advantage over freezer storage through higher DNA recovery. No detectable inhibition of amplification was observed at the recommended SM concentration and complete profiles were obtained from genomic DNA samples even in the presence of higher than recommended concentrations of the SM storage medium. The ability to stabilize and protect DNA from degradation at ambient temperatures for extended time periods could have tremendous impact in simplifying and improving sample storage conditions and requirements. The current work focuses on forensics analysis; however this technology is applicable to all endeavors requiring storage of DNA.     

中国参与经济全球化管理的战略思考——评《陈安论国际经济法学》的主导学术理念

美国金融危机和欧洲部分国家主权债务危机,不仅暴露了西方国家经济体制和运行机制长期存在的经济社会问题,而且也反映了现行国际经济体系已经不能适应新的国际经济形势,构建国际经济新秩序成为当前世界走出危机、重振经济的当务之急。积极参与全球经济治理,推动国际经济体系变革,促进国际经济秩序朝着更加公正合理的方向发展,不仅是我国深化改革开放,为发展营造良好国际环境的需要,而且也是满足国际社会希望中国发挥更大作用的需要。陈安教授在《陈安论国际经济法学》(五卷本)中的许多文章中,特别是在《论中国在建立国际经济新秩序中的战略定位》等4篇系列论文中,从国际经济法学的角度,就我国参与经济全球化管理和推动国际经济秩序变革,提出了一系列独特的战略思想和政策建议,喊出了与时俱进、变法图强的最强音。这是陈安教授30年来一以贯之的学术理念和学术思想,非常值得我们重视和思考。本文结合陈安教授的有关论述,提出了一些中国参与经济全球化管理的战略思考,是为呼应。     

当代土家族婚姻的变迁

本文在田野调查资料的基础上,并利用人口普查资料,主要从定量的角度对土家族传统婚姻文化在当代的变迁进行研究。认为,当代土家族婚姻发生了重大变化,表现为族际通婚和通婚的空间范围有所扩大,近亲婚配在逐步减少;未婚比例大幅度下降,而有配偶率则大幅度上升;早婚有下降的趋势,初婚年龄则呈上升趋势;离婚比例有所增长;择偶标准和方式更加多样化和具时代特征;婚姻仪式简约化;等等。土家族婚姻的变迁带来了家庭的一系列变化,但并未动摇传统家庭的基本结构,传统家庭的相对稳定是土家族社会在当代保持稳定的一个基础。     

Trauma-Informed Resource Parent Training and the Impact on Knowledge Acquisition,Parenting Self-Efficacy,and Child Behavior Outcomes: A Pilot of the Resource Parent Curriculum Parent Management Training (RPC+)

This pilot study illustrates one state’s development, implementation, and outcomes of a trauma-informed curriculum called the Resource Parent Curriculum + CARE (RPC+). The RPC+ is an intensive co-trained curriculum intended to be trauma-informed and skills-based focused on ameliorating child behavior problems that are frequently the source of parenting stress and placement instability. This article will provide a literature review of child and caregiver needs, describe the training curriculum, and present the results from the pilot implementation of the curriculum. Preliminary findings from this study show participants of the RPC+ show positive changes pre- to post-training on a number of dimensions that have been correlated with placement stability for children including knowledge of trauma, parenting self-efficacy, and child behavior outcomes.     

论加强我国普法教育的重要性和必要性

加强普法教育是依法治国、建设社会主义法治国家的基础工程;加强普法教育是服从于、服务于建立、健全社会主义市场经济体制的需要;加强普法教育是促进社会主义精神文明建设的内在要求;加强普法教育是实现国家长治久安、维护社会稳定的重要措施;加强普法教育是提高公民整体素质,坚持科学发展观的需要。     

趋势焦虑与冲突意愿：区域主导权竞争中的防御性进攻主义

区域安全复合体内部主导权竞争常常会引发大国战争。从理性角度讲,倾向于发动主导权战争的国家要么具有"趋势优势",要么具有"实力优势"。但却无法解释为何有些国家既没有"趋势优势",也没有"实力优势",却依然倾向于对"实力较强且趋势占优"的国家发动主导权战争。趋势焦虑不仅可以解释传统理论中守成大国对新兴大国的防御性进攻倾向,更可以解释如果守成大国通过战略打压成功地扭转了新兴大国的崛起趋势,那么新兴大国反而可能因趋势焦虑而选择防御性进攻。避开从传统权力结构性分析路径,而通过引入战略心理学的"趋势焦虑"概念并构建防御性进攻主义的广义理论,将对区域主导权竞争中何者更具进攻性意愿有着更强的解释力和预测力。同时,传统理论认为,霸权国在区域主导权竞争中倾向于依据静态的权力结构性而扶持区域次强国,进而达到抑制权力占优方的目标。但从战略势能演变的动态性视角分析,霸权国在区域主导权竞争中并非一贯倾向对区域次强国予以支持,而是更倾向对"趋势占劣"或"战略匹配高"的地区大国予以支持,哪怕其权力尚处于优势地位。     

服务型政府能力建设新途径:战略管理

战略管理作为提升政府能力的工具之一，有助于服务型政府实现有效性的目标，达到对组织能力的变革，是一科，新的政府能力建设途径。     

CJTF与北约军事战略的改革

本文介绍和分析了冷战后北约战略调整过程中出台的一项重要政策--建立多兵种联合特遣部队(Com-bined Joint Task Force,CJTF).CJTE是一种为特定紧急行动组织的、能够提供多种服务的武装力量.其组建方式类似"搭积木",指挥人员构成"内核",人员与装备构成"模块","内核"与"模块"平时就"隐藏"在北约军事一体化组织中,根据不同任务将不同的"内核"与"模块"组合起来,迅速投入到危机处理或集体防御行动中,又被称为"乐高"兵团.DJTF的建立是北约为改善危机处理能力、调整美欧关系、解决中、东欧国家参与北约行动的重要举措,是冷战后北约军事战略调整的组成部分.     

率实施青少年治安战略工程

青少年犯罪问题是历史性的顽症.迫切需要构建预防青少年犯罪的战略工程.即提上战略地位,提出战略对策,统一战略思路,落实战略措施.当前的问题是在许多有关部门未能摆上实际的战略地位;对这方面的研究也相当薄弱.预防青少年犯罪要突出德育.在影响青少年犯罪的意识因素中最重要的是伦理道德.我国虽对道德高度重视,但是落实上有很大差距.预防青少年犯罪要将司法对策与社会对策相结合,将对未成年人的"保护"与"预防犯罪"相结合,实现系统工程化.建立整体性领导与执行机构;统一指导意识;依靠多元动力;强化反馈控制机制.     

经济全球化环境中的动态创新组织

在以日益复杂化且变化迅速为特征的新的经济全球化环境中 ,企业的生命力和持续竞争优势将越来越多地依赖于更有效、更快速的动态创新能力 ,成长快速的公司和市场领导者的成就 ,往往不是来自于低成本的“收获战略” ,而是善用知识的力量执著于持续创新的事业。