In this report, the bodies of six skinned animals were submitted to the Oklahoma Animal Disease Diagnostic Laboratory for necropsy examination as authorities were concerned these animals were domestic dogs. Given the condition of the animals, identification of the animal species could not be based on traditional physical characteristics such as size, fur characteristics, and ear characteristics. The paw prints from these animals were characterized based on size, heel pad architecture, and claw characteristics. The length of the forepaws measured 3-3.25 in., four toes were observed and only the claws of digits 3 and 4 were identified, and the heel pad had a pronounced three-lobed rear margin. The rear margin of the heel pad of the hind foot was of circular shape with two slightly forward-pointing crescents laterally. Based on the features of the paw print it was determined that the animals necropsied were coyotes. 相似文献
Although most DNA crime laboratories may not encounter fecal samples often, they are a familiar sample type in non‐human forensic laboratories due to their prevalence in the environment. Fecal matter can be challenging due to low numbers of nucleated cells and the presence of inhibitors that impede amplification success. Sampling location (internal vs. external), sampling quantity (10–200 mg), and various extraction protocols (silica matrix, bead beating, and clean‐up column) were evaluated to maximize DNA yield. The greatest yield of intact DNA was obtained using a modified silica matrix extraction protocol (VGL‐Fecal) on 30–50 mg of fecal matter collected from the external surface of a stool that had been dried for 24 h. This optimized sampling and extraction protocol was applied to a pilot study where DNA yield and genotyping success were evaluated. By optimizing our collection, sampling, and extraction procedures, a reliable method for maximizing the yield of canine fecal DNA was developed. 相似文献
The discriminatory power of the noncoding control region (CR) of domestic dog mitochondrial DNA alone is relatively low. The extent to which the discriminatory power could be increased by analyzing additional highly variable coding regions of the mitochondrial genome (mtGenome) was therefore investigated. Genetic variability across the mtGenome was evaluated by phylogenetic analysis, and the three most variable ~1 kb coding regions identified. We then sampled 100 Swedish dogs to represent breeds in accordance with their frequency in the Swedish population. A previously published dataset of 59 dog mtGenomes collected in the United States was also analyzed. Inclusion of the three coding regions increased the exclusion capacity considerably for the Swedish sample, from 0.920 for the CR alone to 0.964 for all four regions. The number of mtDNA types among all 159 dogs increased from 41 to 72, the four most frequent CR haplotypes being resolved into 22 different haplotypes. 相似文献
While police work comes with a slew of dangers, little research has focused on deaths of police dogs in the line of duty. The purpose of this paper is to begin a conversation about violence toward police dogs and the treatment of animals working with the police.
To do so, a database of 96 police dogs that died in the line of duty in the United States between 2011 and 2015 was compiled, drawn from the Officer Down Memorial Page. More police dog deaths were reported in 2014 and 2015, during summer months, with half clustering in the Southern US. The victim police dogs were mostly younger and recently employed by the policing agency. The most frequent cause of death was heat exhaustion, followed by gunfire and automobiles. Most offenders were apprehended by police or shot.
Policymakers and researchers should encourage systematic data collection for a better understanding of the extent of the issue. 相似文献
Research to counter the threat of organic peroxides such as triacetone triperoxide (TATP) is at times hampered by their inherent extreme sensitiveness and volatility. This work describes an approach to lowering some risks associated with the handling of TATP in the laboratory through the use of an analog species, tripentanone triperoxide (TPTP). Sensitiveness has been tested via standard methods. GCMS analysis has shown that TPTP degrades via similar mechanisms to TATP under a range of conditions. Slight differences in product composition were traced to side reactions which may also affect impurities present in homemade TATP synthesis. A pilot field trial was conducted to evaluate TPTP as a substitute for TATP in explosive detection dog (EDD) scent training. The degradation studies have yielded insights into the complexities of the acidic degradation of cyclic peroxides with potential forensic application, and TPTP's inadequacy as a TATP pseudoscent is a valuable example of the limitations of such training aids. 相似文献