CDK5的表达与脑损伤时间推断的相关性研究进展

细胞周期蛋白依赖激酶5(cyclin-dependent kinase 5,CDK5)是CDK家族中一类不直接调控细胞周期的蛋白。CDK5通过靶蛋白的磷酸化在神经细胞的发育、修复及退行性变等方面发挥着不可替代的作用。脑损伤是指暴力作用于头部造成的脑组织器质性损伤,由于损伤发生后机体启动应激及修复机制,脑组织中变化的多种蛋白及酶类物质可作为损伤时间判定的指标。本文就CDK5的分布、活性机制、生理作用及其脑损伤研究与其作为脑损伤标志物可能性的研究进展进行综述。     

简论当代中国社会转型及其对法治化的影响

当代中国社会处于由传统向现代转型的深刻变革过程中。社会转型是社会在整体意义上与质的意义上的嬗变。社会转型在各种利益与关系的冲突撞击中追求社会朝法治方向发展 ,同时转型时期的社会环境也为法治的发展提供了契机。     

Do party leadership contests forecast British general elections?

When assessing election forecasts, two important criteria emerge: their accuracy (precision) and lead time (distance to event). Curiously, in both 2010 and 2015 the most accurate forecasts came from models having the longest lead time—albeit at most 12 months. Can we increase the lead time further, supposing we tolerate a small decrease in accuracy? Here, we develop a model with a lead time of more than 3 years. Our Party Leadership Model relies on the votes of MPs when selecting their party leader. We assess the forecasting quality of our model with both leave-one-out cross-validation and a before-the-fact forecast of the 2019 general election. Compared to both simple forecasting methods and other scientific forecasts, our model emerges as a leading contender. This result suggests that election forecasting may benefit from developing models with longer lead times, and that party leaders may influence election outcomes more than is usually thought.     

IMMIGRATION AND THE RECENT VIOLENT CRIME DROP IN THE UNITED STATES: A POOLED,CROSS‐SECTIONAL TIME‐SERIES ANALYSIS OF METROPOLITAN AREAS*

A good deal of research in recent years has revisited the relationship between immigration and violent crime. Various scholars have suggested that, contrary to the claims of the classic Chicago School, large immigrant populations might be associated with lower rather than higher rates of criminal violence. A limitation of the research in this area is that it has been based largely on cross‐sectional analyses for a restricted range of geographic areas. Using time‐series techniques and annual data for metropolitan areas over the 1994–2004 period, we assess the impact of changes in immigration on changes in violent crime rates. The findings of multivariate analyses indicate that violent crime rates tended to decrease as metropolitan areas experienced gains in their concentration of immigrants. This inverse relationship is especially robust for the offense of robbery. Overall, our results support the hypothesis that the broad reductions in violent crime during recent years are partially attributable to increases in immigration.     

过度机械通气致肺损伤TNF-α、IL-1β及NF-κBp65的表达

目的探讨过度机械通气致肺损伤后,炎症因子TNF-α、IL-1β及NF-κBp65在肺组织中不同时间表达的变化规律。方法将51只雄性新西兰大白兔分为正常组、对照组、实验组。利用呼吸机建立过度机械通气致肺损伤模型。应用免疫印迹技术观察兔急性肺损伤后肺组织中TNF-α、IL-1β及NF-κBp65的表达,并用凝胶图像处理系统进行分析,所得数据经SPSS 10.0统计软件处理。结果正常组与各对照组之间比较,均无明显差异(P>0.05)。TNF-α在损伤后0h表达即增加,1h达高峰,以后逐渐下降,而在24h后又有反弹性增高;其中在0.5、24、48h组有差异性(P<0.05),1、3h组有显著差异性(P<0.01)。IL-1β在损伤后0.5h逐渐增加,3h达高峰,以后逐渐下降,其中在1、6、12、24h组有差异性(P<0.05),3h组有显著差异性(P<0.01)。NF-κBp65在损伤后0.5h逐渐增加,6h达高峰,以后逐渐下降,其中在1、12h组有差异性(P<0.05),3、6h组有显著性差异(P<0.01)。结论TNF-α、IL-1β及NF-κBp65在过度机械通气致VILI发病中有重要作用;TNF-α、IL-1β及NF-κBp65在损伤早期即表达,且在时间上有一定的变化规律,可为损伤时间的推断提供参考。     

民事权利限制的时间界限

权利限制是对权利范围和权利行使所作的限制性规定。私法视野下,权利限制是确保权利人正当行使权利的需要。民事权利的时间界限是权利限度的形式之一,民事权利限制的时间界限,表现为宏观和微观两个方面。民事权利的时间界限的合理性和二元性有利于实现个人利益和社会利益的统一。     

李大钊早期传播马克思主义的再探讨

五四前后李大钊通过办报办刊、发表论著、组织社团、办学讲课、演讲辩论等方式向中国民众传播了马克思主义。他早年对马克思主义的研究和宣传主要涵盖了四方面内容：揭露了帝国主义的本质,阐述了十月革命的伟大意义;较系统地论述了马克思主义理论的基本内容;正确论述了中国革命的一些基本问题,如革命的步骤、手段、动力等等;号召学生和知识分子走与工农相结合的道路。这些杰出的思想主张形成了他早年的理论特色。当然,李大钊对马克思主义的最初理解也存在着一些不准确之处,这种认识偏差是与当时他所处的环境和背景分不开的。李大钊的可贵之处在于其思想随着社会的进步不断发展,并及时将先进的科学理论运用于中国社会变革的实践。     

行政许可程序的立法欠缺及其完善

行政许可程序是规范行政许可的行为,防止行政许可实施权的滥用,保障公民、组织在行政许可实施中的正当权利的重要环节。尽管行政许可法设计了许多程序规则,但在某些方面还存在问题,诸如处理期限的规定不完善、听证程序不健全、缺乏有效的保障制度等。笔者在对此分析研究的基础上,提出了一些健全和完善我国行政许可程序的设想和建议。     

A real-time multiplex SNP melting assay to discriminate individuals

A method that quickly and inexpensively differentiates crime scene samples from multiple donors would expedite casework analysis by allowing the selection of probative items requiring comprehensive testing. This new method need not be perfectly definitive nor give a complete 13 locus short tandem repeat (STR) profile; it simply must be able to differentiate between most victim and suspect samples. We describe the development of multiplex, single nucleotide polymorphism (SNP), fluorescence resonance energy transfer-based real-time polymerase chain reaction (PCR) assays to fulfill this need. Dual probes, one fluorescently labeled and the other labeled with a quencher, are monitored during a melt analysis to reveal an increase in fluorescence, which allows the assessment of the two SNP alleles. Two alternate 6-plex assays (with and without gender determination) have been developed for the six-color RG6000 real-time instrument (Corbett Robotics, Inc.) and one seven SNP plus gender assay (performed as two 4-plex assays, one with gender the other without) have been developed for use in four/five color real-time instruments. This technique can discriminate between 95% and 99% of samples from different individuals. This assay is fast (approximately 2 h), much less expensive than STR analysis, and uses a real-time PCR instrument which is found in most forensic and molecular biology labs.     

大鼠弥漫性轴索损伤后NGF表达

目的研究大鼠弥漫性轴索损伤后神经生长因子（NGF）表达的时间变化规律及意义。方法采用SD雄性大鼠复制弥漫性轴索损伤（DAI）模型,分别于伤后1、3、6、12、24、48h和3、7d取脑组织,应用免疫组织化学法对DAI后不同时间段大脑皮层、丘脑、小脑和海马部位NGF的表达变化进行观察并与正常组、假手术组对照。结果正常及假手术组大鼠大脑皮层、丘脑、小脑、海马均有少量NGF的表达。打击后1hNGF表达增强,12h达高峰,3d开始下降,7d降至正常。结论NGF参与弥漫性轴索损伤后的修复：NGF的时序性变化有望成为法医学脑损伤时间推断的客观指标。