抗弓形虫MIC3单抗的制备及其在速殖子免疫荧光观察中的应用

为开展弓形虫速殖子阶段蛋白表达和定位的相关研究,利用弓形虫重组微线体蛋白3(MIC3)抗原研制单克隆抗体,筛选出适合在虫体内用免疫荧光观察该蛋白的单抗分泌株,建立了弓形虫间接免疫荧光观察方法。结果显示,经4轮亚克隆后得到3株稳定分泌抗MIC3单抗的分泌株:4G1、1G12和5D7;经测定,3株MIC3单抗4G1、1G12和5D7的亚型分别为IgG1、IgG2b、IgG2b,效价分别为1∶3 200、1∶12 800、1∶1 600。筛选得到的2株MIC3单抗(4G1和1G12)可用于间接免疫荧光检测MIC3蛋白在虫体中的分布。通过弓形虫阳性感染猪血液样品推片的免疫荧光试验,验证了这3株单抗具有特异性。结果表明,研制的MIC3单抗和虫体荧光观察体系可用于速殖子MIC3蛋白在虫体表达定位和其他研究,也可作为其他速殖子抗原荧光观察的比较对象。     

经济全球化:发展中国家的机遇与挑战

经济全球化已成为不可阻挡的世界性潮流,不管我们喜欢它也好,不喜欢它也好,它都实实在在地影响和改变着我们的生活,任何国家、民族都无法独立于经济全球化之外.由于经济全球化是资本主义主导下的历史进程,是资本主义生产方式的必然延伸,因此,广大的发展中国家在这一过程中处于相对不利的地位,面临较大困难.但它们必须成为这个过程的一部分,抓住机遇、迎接挑战,扬长避短、趋利避害.此外别无选择.     

信息产业发展对于甘肃省总体经济发展贡献水平分析

本文通过对国内外信息产业发展的分析比较,着重讨论了甘肃省信息产业发展的情况,并分析了与甘肃省国民经济发展的适应性,讨论了信息产业对于经济发展的贡献水平,提出了甘肃省信息产业发展中应该重视的问题。     

为什么说“只管不收”是工商行政管理的改革目标

工商行政管理的“收管混台”使“重收轻管”一直未得到解决 ,在其“垂直改制”后仍面对收“两费”的现实。由于“收”与“管”是两种不同的运行主体 ,再结合工商行政管理的管理活动属性及目前的财政体制改革来看 ,工商行政管理应是“只管不收”。     

吉林省证券业发展对策研究——基于“十一五”期间数据

"十一五"以来,吉林省证券业试图壮大上市公司队伍,通过买壳、借壳等多种方式扩大直接融资的规模。但由于各种原因,辖区上市公司数量不但没有增加,反而减少。与国内发达省区比较依然存在一些差距。针对吉林省证券业现状出现的问题,应加快培养本地综合类券商或引进综合类券商推进重组工作、提高上市公司再融资能力、加快培育上市公司后续资源、力争长春高新技术产业开发区进入代办股份转让系统等对策。     

马来西亚汽车市场分析：与泰国汽车市场比较

在中国-东盟自由贸易区（CAFTA）正式启动的宏观背景下,本文对马来西亚汽车市场进行详细的总量和结构分析,指出近10年来马来西亚汽车工业保持稳定的发展速度,但泰国汽车工业的迅速崛起使马来西亚汽车产业失去了在东盟的主导地位;而马来西亚政府推行的进口替代汽车产业政策和国民车计划直接决定了该国汽车产业的发展动向。在实证分析部分,我们通过显性比较优势指数RCA测算发现马来西亚汽车产业不具备显性比较优势,通过恒定市场份额CMS分析得出2001—2007年间虽然马来西亚汽车产业出口竞争力呈上升趋势,但竞争力呈自我弱化趋势。与泰国汽车产业的测算结果比较反过来验证了泰国政府及时顺应世界贸易自由化浪潮进行产业政策转变的正确性。     

美国金融危机对泰国金融业的影响

作为1997年亚洲金融危机的始发国和重创国,泰国金融业在此次金融危机中受到的影响程度备受关注。本文在金融危机传导机制的基础上分析了美国金融危机对泰国金融业的影响,并探索对我国金融业健康发展的一些启示。     

北京奥运助推我国体育产业的全面提升和发展

奥运不仅是体育盛会,也是世界经济的大舞台,奥运经济为整个体育产业造就了巨大的商机,因此,紧紧抓住这一历史发展机遇,大力发展体育产业,全面实施奥运经济战略,从而助推我国体育产业的全面提升和发展。     

Perceptions of the Regulated Community in Environmental Policy: The View from Below

Discussions in environmental policy often focus on the highest levels of decision making and action while paying scant attention to those individuals on the front lines. Among those frequently overlooked are the individuals at regulated facilities who interact with government regulators on a frequent basis. Interviews with nearly two dozen facility personnel in Virginia yield findings that challenge common perceptions of the relationships between facility personnel and inspectors. In particular, 86 percent of facility personnel, representing a range of regulated facilities from prisons to landfills to dry cleaners, said their interactions with inspectors were positive. Approximately 70 percent of them said that they trust inspectors and provided evidence of trust in their stories. The ramifications of these findings for environmental policy could be potentially significant because facility personnel are presumed to be adversarial, if not outright hostile, and this assumption impacts the design and implementation of environmental regulations.     

Leveraging liminality: how San Diego taxi drivers used their precarious status to win reform

ABSTRACT

Using a case study in San Diego, California, we explore the complexities of precarious employment for taxi drivers. We seek to answer the following question: how do the ambiguities of taxi drivers’ status as independent contractors shape drivers’ work conditions and opportunities for resistance? Our study is based on 331 surveys, 20 in-depth interviews, participant observation, and policy analysis over two years. While drivers were objectively disempowered by the independent contracting designation, lacking both the protections granted employees and the prerogatives of ownership, they were empowered by the alliances this duality facilitated. Drivers used their marginal identities as workers and as entrepreneurs to their advantage in their campaign for reform. This case presents an alternative narrative to previous research, which generally highlights how independent contractors either accept their status in exchange for flexibility or resist by claiming misclassification. The immigrant taxi drivers in our case study actively resisted, not by pursuing recognition as employees, but rather by successfully seeking the full rights of business owners.