鸭源新城疫强毒株对番鸭细胞因子及Toll样受体mRNA表达的影响

为研究人工感染鸭源新城疫强毒株(Md/CH/LGD/1/2005)诱导番鸭细胞因子及Toll样受体基因的表达情况,选用80只15日龄SPF番鸭,随机分为攻毒组和对照组,每组各40只,攻毒组以滴鼻点眼的方式接种1×107.0EID50鸭源基因Ⅶ型新城疫病毒(NDV)Md/CH/LGD/1/2005,对照组接种灭菌的磷酸盐...     

中药对鸡新城疫灭活疫苗免疫效果的影响

手工乳化制备含 2 0 g/L人参皂甙、4 0g/L海藻多糖、4 0 g/L黄芪多糖、4 0 g/L芦荟多糖的鸡新城疫灭活油乳剂疫苗 ,注射免疫 2 1日龄粤黄鸡 ,即日起连续 3d按体重胸肌注射环磷酰胺 4 0mg/kg ,每日 1次 ;免疫后第 7、14、2 1、2 8、4 2d采血检测鸡新城疫HI抗体效价 ,免疫后第 14、2 8d采血检测红细胞CR1受体 (RBC CR1)花环率、免疫器官指数和血清超氧化物歧化酶 (SOD)活性。结果表明 ,人参皂甙、海藻多糖在免疫后期能提高鸡新城疫HI抗体效价 ,而黄芪多糖、芦荟多糖作用不明显 ;人参皂甙、黄芪多糖、芦荟多糖能显著提高鸡RBC CR1花环率 (P <0 .0 5 ) ,海藻多糖作用不显著 (P >0 .0 5 ) ;海藻多糖、芦荟多糖能显著提高血清SOD活性 (P <0 .0 5 ) ,人参皂甙、黄芪多糖作用不显著 (P >0 .0 5 ) ;人参皂甙、海藻多糖能显著提高免疫器官指数 (P <0 .0 5 ) ,黄芪多糖、芦荟多糖作用不显著 (P >0 .0 5 )。     

口蹄疫病毒3D蛋白对DNA疫苗免疫效果的影响

将携带O型FMDVChina 99株P1 2A、部分 2B及 3C蛋白酶编码基因的真核表达质粒与在毕赤酵母中表达的纯化FMDVChina 99株 3D蛋白同时经肌肉注射方法接种豚鼠。以MTT法检测豚鼠脾淋巴细胞经植物血凝素 (PHA)刺激后的增殖活性 ,以间接ELISA方法检测血清FMDV特异抗体变化 ,并以微量中和试验检测中和抗体水平。结果表明 ,FMDDNA疫苗与 3D蛋白共同免疫豚鼠后 ,抗体水平没有明显提高 ,攻毒后的保护率为 2 5 %。     

人参注射液治疗冠心病心气虚证与心阴虚证的临床研究

We have researched clinical effect of treated 57 cases of heart-Qi deficiency and heart-Yin deficiency of coronary heart disease with Ginseng injections. The results indicate Ginseng can improve angina pectorss and electrocardiogram,hemorrheogical indices,external thrombogenesis,microcirculation s'' abnormality; degrease symptom indices of heart-Qi deficiency of coronary heart disease remarkablely. But Ginseng have worse effect of angina pectoris and electrocardiogram to heart-Yin deficiency than to heart-Qi;deficiency of coronary heart disease; and symptom indices. hemorrheogical indices, external thrombogenesis, microcirculation in heart-Yin deficiency worsen. These prove there must be syndrome-differentiation in treatment of coronary heart disease with Ginseng injections.     

肝豆状核变性肝脏声像图特征的再探讨

１４０例肝豆状核变性的肝脏声像图特征分为７型；脂肪均匀浸润型，脂肪非均匀多灶浸润型，脂肪非均匀弥漫浸润型，光点光烁型，岩层征型，树枝状光带型和结节型。ＨＬＤ多样面肌痉挛是复杂的肝脏声像图特征与詹病程发展的不同阶段有关，反映肝 铜沉积由轻到重的病理过程，有助于对ＨＬＤ的诊断和病情分析。     

“Our biggest killer”: multimodal discourse representations of dementia in the British press

A recent (2016) Office for National Statistics report stated that dementia is now “the leading cause of death” in England and Wales. Ever fixated with the syndrome (an unfailingly newsworthy topic), the British press was quick to respond to the bulletin, consistently headlining that dementia was the nation’s “biggest killer,” while (re)formulating other aspects of the report in distorting and emotive metaphorical terms. In this paper we examine how the media, through use of a recurring set of linguistic and visual semiotic tropes, portrayed dementia as an agentive entity, a “killer,” which remorselessly attacks its “victims.” Such a broadly loaded and sensationalist representation, we argue, not only construed dementia as a direful and pernicious disease, but also, crucially, obscured the personal and social contexts in which the syndrome is understood and experienced (not least by people with dementia themselves). This intensely lurid type of representation not only fails to address the ageist misinformation and common misunderstandings that all too commonly surround dementia, but is also likely to exacerbate the stress and depression frequently experienced by people with dementia and their families.     

口蹄疫A型灭活疫苗毒种和种子批的研究

为了保证制苗及攻毒用毒种的质量和稳定性,在对制苗和攻毒毒种的毒力、特异性、保存期、扩繁代次等进行研究的基础上,建立了口蹄疫A型灭活疫苗制苗和攻毒用毒种种子批。结果表明,原始毒种和基础毒种的扩繁代次均控制在5代以内,适宜的保存方法和保存期均为加含 500 mL／L甘油的PBS后在-30℃保存1年、-70℃保存2年;用于生产抗原的工作毒种,最高扩繁代次控制在15代以内,最佳保存方法和保存期为加保护剂后在-20℃保存半年、-70℃保存1 年;用于效力检验的攻毒毒种采用原始毒种。通过制苗和攻毒用毒种种子批的建立,规范了口蹄疫疫苗生产和检验过程中的毒种管理。     

复方丹参滴丸治疗冠心病39例

影察复方丹参滴丸治疗冠心病心绞痛等的疗效，初步揭示该药的临床作用机理。方法：３９例冠心病患者行冠状动脉造影术，并按统一标准进行中医辨证，观察配服复方丹参滴丸后血分数等的关系。     

论精神分裂症与外力致伤的关系：从一例司法精神鉴定案的始末谈起

司法精神鉴定具有特定的复杂性,尤其是对于外力致伤程度的评定应严格把握好外伤所致精神病与外伤所诱发的精神病的鉴定标准。     

卫生知识与性别分工——近代中国传染病知识的性别化传播研究

女性是近代传染病知识传播的重要受众。得益于近代中国对传染病防治的日益重视和"家庭"在传染病防治中的特殊地位,妇女报刊和以教科书为基础的学校教育将较为系统前沿的传染病知识传播给女性。同时,通俗实用的传染病知识也经由民众教育向普通城乡妇女传播。在近代传染病知识向女性传播的过程中,性别化传播始终存在。传统性别分工成为向女性传播传染病知识的重要依据,性别化传播也在一定程度上强化了传统性别分工。但在缺乏医学启蒙的近代中国,围绕"家庭"的知识传播,向女性提供了必要的传染病防治知识。近代中国重视以家庭为单位的传染病知识传播策略,可以给当下提升城乡居民传染病防治素养以启示。