Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse

A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4‐methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 μL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology.     

“未成年人前科消灭“的理论与实践探讨

建立"未成年人前科消灭"制度具有重要的理论和现实意义,它既符合法的正义价值,又有利于实现刑罚的目的,同时还有利于撕掉犯罪标签和完善我国现有的未成年人保护法律体系.但目前在我国实施还面临一些困境,即该制度与我国现行相关法律冲突,制定主体不适格,缺乏相应的法律支持和配套实施机构,极易导致司法腐败.因此,只有在充分的法律准备下才能在全国推广和实施.     

行政诉讼法修改三题

现行行政诉讼法的全面修改必须依托于广泛的理论争鸣和深厚的学术积淀。为此,必须深入总结行政诉讼法实施的教训,并以此作为法典修订的现实基础,准确把握行政诉讼制度的根本目的,并以此作为法典修订的灵魂,敏锐洞察全球行政诉讼制度发展的规律作为法典修订的镜鉴。     

英美法中诉因记载的法定要求及其借鉴意义

诉因记载的法定要求经历了从严格形式主义到实质主义的转变,诉因记载的法定要求表现在诉因记载的特定性、诉因的单一性、诉因的选择与预备记载、诉因的合并记载上。我国现行法未对起诉书的记载做出具体规定,诉因记载的法定要求为我国规定如何记载起诉书提供了借鉴。     

论刑法中的“着手”——以抢劫罪为视角

“着手”是刑法中的一个重要概念,其本身不仅是实行行为的起点,而且还是区分犯罪预备与未遂的重要标志。对行为是否为“着手”的判断,不应仅从构成要件的形式角度,还应从行为对法益侵害的实质角度来理解。     

“后奥运”时期重大国际性活动涉外安保工作的几点思考

“后奥运”时期我国将迎来越来越多的重大国际性活动,如何更好地做好涉外安保工作,是值得我们研究的重要课题。重大国际性活动具有规模大,规格高,参与人员数量众多,易形成场地饱和状态,相关活动多,社会影响广泛等特点。重大国际活动涉外保卫工作的相关事前准备包括思想准备、物质准备、事前实务准备、警力部署等。对重大国际活动性涉外案（事）件的处理,是一项政治性、法律性、原则性很强的工作,也是重大国际性活动涉外安保工作的中心环节,因此,应采取相应措施予以精心处置。     

Identification of biological samples in a case of contamination of a cytological slide preparation

Here we report a case where a discrete section of the cytological slide preparation of a female individual was obviously contaminated with pleura liquid of a female tumor patient. Analysis of the cancerous pleura liquid and the healthy cells of the slide preparation showed different DNA profiles, indicating that the material originated from two different female individuals. The DNA profile of the cell mixture revealed a heterogenous pattern whereby the alleles could be assigned to the healthy and the tumor patient. Loss of heterozygosity (LOH) was observed in four of eight short tandem repeat systems for the pleura liquid and the cell mixture. Despite the low amount of DNA on the slide preparation and the occurrence of LOH, it was possible to clarify the case and to support the assumption that a drop of cancerous pleura liquid contaminated the cytological slide.     

重组猪IgGⅡB类Fc受体蛋白的原核表达和抗体的制备

根据GenBank中猪IgGⅡB类Fc受体剪接异构体cDNA(swFcγRⅡB)基因序列(FJ608551),利用Primer5.0软件设计合成了1对特异性引物,扩增swFcγRⅡB去掉胞内区和跨膜区的基因,PCR产物经KpnⅠ+EcoRⅠ双酶切后,将截短的swFcγRⅡB基因亚克隆到原核表达载体pET-32a中构建了重组表达质粒pET-swFcγRⅡB,在IPTG诱导下成功表达了分子质量约为40ku的融合蛋白swFcγRⅡB-His,该表达蛋白在细胞质中主要以不溶性包涵体的形式存在。原核表达蛋白经纯化免疫小鼠,制备鼠源swFcγRⅡB封闭抗体,ELISA检测抗体效价为1:5120,具有高度特异性。Western-blotting检测表明,制备的多克隆抗体可以与融合蛋白swFcγRⅡB-His特异性结合。     

液相微萃取在法庭科学中的研究进展

液相微萃取集采样、萃取、富集于一体,具有成本低,溶剂用量少,易与高效液相色谱、气相色谱、毛细管电泳、质谱等多种分析仪器联用等特点。而且回收率和富集效率都较高,样品的净化功能强大,特别适用于物证检材中痕量、超痕量目标分析物的提取。本文对液相微萃取原理、装置和操作模式以及近年来在物证分析中的研究进展进行了综述。     

扩张中的犯罪预备及参与形式——围绕第18届国际刑法学大会第一专题的展开

新的社会经济形势下,恐怖主义及其它严重有组织犯罪在世界范围内日益蔓延,作为针对此类犯罪现象的积极调整,各国刑事立法与司法同步呈现出扩张的趋势。着眼刑事实体法层面,不难发现不同形式的预备行为及参与行为之犯罪化处遇同样有着相当的膨胀,此处结合第18届国际刑法学大会第一专题之讨论试图对该命题作进一步的展开。