国产磁珠Wawasye试剂盒在法医DNA中应用

目的探讨国产磁珠Wawasye试剂盒在法医DNA检案中的应用效果。方法利用475份各类常见生物检材测试其效果,并与M48磁珠试剂盒作比较。结果国产磁珠Wawasye试剂盒与M48磁珠试剂盒在检验各类常见生物检材的结果无显著性差异。结论国产磁珠Wawasye试剂盒适用于公安机关DNA实验室。     

毕节试验区劳务经济研究

毕节试验区成立以来,中央统战部暨各民主党派团结合作共谋发展,以探索科学发展理念路径和实践为使命,把单纯为解决人口压力和吃饭问题的劳务输出转变为发展参与要素收益分配的劳务经济,取得了显著成效.通过对毕节试验区发展劳务经济的实践经验进行理论梳理和总结,为进一步发展劳务经济、推进城镇化建设、不断探索"毕节模式"推动试验区新一...     

应用型本科院校新生心理健康状况的调查与分析——以黑龙江工程学院2010级新生为例

通过采用大学生人格健康问卷(UPI)对黑龙江工程学院2010级2475名入学新生进行心理测试,从入学新生总体和男女生个体两个维度进行分析.结果表明:黑龙江工程学院入学新生的心理健康状况与国内其他类型大学UPI筛查率差异不大,但也有15.2%的学生可能存在一定心理问题或障碍.数据结果显示:被试男女生心理健康水平具有显著差...     

论我国犯罪心理测试法律规范的构建

近年来,犯罪心理测试技术越来越多地应用于我国的案件侦查和司法调查,但其法律地位不明确。实践中缺乏统一、标准化的操作规范,对犯罪心理测试结论的审查、采用规则,阻碍了犯罪心理测试技术的良好运用。应以犯罪心理测试的技术要求为基点,以其他证据的法律要求规范为参考,构建犯罪心理测试运用的程序规范和测试结论运用规则。其程序规范为：对犯罪心理测试的有限使用;测试的适用主体限制;权利告知制度;测试的不受干扰原则。     

DETECHIP®: A Sensor for Drugs of Abuse*

Abstract: The design and preliminary characterization of a novel sensor for drugs of abuse, DETECHIP^®, is described in this proof‐of‐concept note. Combining both colorimetric and fluorimetric assays, DETECHIP^® is suitable for lab and field use. More than a conventional spot test which provides a single “yes or no” answer, DETECHIP^® provides twenty responses for a more complete characterization of suspect material. This is accomplished by visually noting colorimetric and fluorescent changes of carefully selected dyes upon the addition of test analytes, including drugs of abuse, with respect to controls. Color and fluorescence changes are recorded numerically so that a 20 digit identification code can be constructed for comparison of test analytes and known compounds. DETECHIP^® is applicable to a variety of drugs, both plant‐derived and synthetic, addressing the need to use several different spot tests simultaneously for a single sample.     

Sensitivity of the Luminol Test with Blue Denim

Abstract: An article appearing in this journal in 2000 suggested that the sensitivity of the luminol test performed on denim fabric is usually no greater than at a 1:100 dilution of blood. This study shows that the luminol test may be unambiguously interpreted at substantially greater dilutions of blood. In this study, four different types of denim were tested by spraying a swatch of fabric with a typical formulation of the luminol reagent. Testing was conducted of dilutions of blood up to 1:1000, all of which showed distinct chemiluminescence. Diluted blood was applied to denim material in the form of a random number. A successful test was obtained only when a “blind” observer, i.e., an observer who was uninformed of the number, correctly reported the number.     

食品安全地方立法的重大疑难问题解析——以起草《重庆市食品安全条例》为视角

《中华人民共和国食品安全法》以及《中华人民共和国食品安全法实施条例》的颁布实施,为完善我国食品安全监管体制,改善我国食品安全现状,充分保障公众身体健康和生命安全翻开了新的篇章。笔者于2008年8月受托主持起草并参研重庆市人民政府法制办公室委托起草项目《重庆市食品安全条例》,课题组历时1年,通过深入考察调研、广泛征求意见、经过8次修改,终于完成该《条例》起草工作。该项目已于2009年9月通过立法评审并结题。根据在《条例》起草过程中遇到的重大疑难问题,在食品安全监管模式方面,建议在坚持现有的多部门分段监管的基础上,建立并完善食品安全监管部门之间的协调机制、加大机构之间的协调力度;在食品安全监管部门的职责划分方面,建议对食品安全监管部门的职责划分作出明确、清晰的细化规定;对食品安全风险监测作出了突出监管重点的制度设计,规定风险评估的情形及安全隐患的处理机制;此外应明确各监管部门对"五小"食品的监管职责,构建食品检验结果信息共享机制。     

我国环境规制与经济增长关系的区域差异分析——基于东、中、西部面板数据的实证研究

根据1998—2006年中国30个省区的面板数据,运用相关的计量经济学方法,对我国东、中、西部地区环境规制与经济增长的关系进行实证研究的结果表明,我国环境规制水平与经济增长的关系呈现出明显的区域差异性。长期内,东、中、西部地区环境规制水平与经济增长均互为因果关系,短期内东部地区环境规制水平对经济增长影响显著,中部地区两者之间关系不显著,而西部地区经济增长在一定程度上推动了环境规制水平提升。据此应当采取相应的政策措施,以促进中国各区域环境规制与经济增长的良性互动和协调发展。     

三聚氰胺对小鼠的致突变及致畸作用

选用昆明种小白鼠为研究对象,进行了三聚氰胺微核试验、精子畸形试验及致畸胎试验,以评价其致突变及致畸作用。结果显示,三聚氰胺在295.87～1 479.35mg/kg剂量范围内,小鼠骨髓嗜多染红细胞微核率及PCE/NCE比值与阴性对照组无差异性(P>0.05);三聚氰胺中剂量组(591.74mg/kg)和高剂量组(1 479.35mg/kg)小鼠精子畸形率显著高于阴性对照组(P<0.05),精子畸形类型以折尾为主;三聚氰胺在19.72～98.62mg/kg剂量范围内,对孕鼠的生殖机能以及胎鼠的生长发育、外观、骨骼和内脏畸形无影响。结果表明,在本试验设计的剂量范围内,三聚氰胺无骨髓细胞毒性及致突变作用,对小鼠无胚胎毒性及致畸作用,但有精子致畸作用。     

Comparison between Temperature Gradient Gel Electrophoresis of Bacterial 16S rDNA and Diatom Test for Diagnosis of Drowning

When a body is discovered in water, it is difficult to conclude whether the cause of death was drowning, even today. Although diatom testing by the digestive method is classical, we hypothesized that aquatic bacteria, as well as diatoms, might be detected in drowned bodies, and conducted temperature gradient gel electrophoresis (TGGE)‐targeting 16S rDNA. DNA was extracted from the site water, and from heart blood and liver samples from 27 bodies concluded as drowning deaths by autopsy and subjected to TGGE after amplification of 16S rDNA by polymerase chain reaction. We observed whether the feature point of each 16S rDNA from the site water and blood or liver samples matched. Considerably higher correspondence was observed in drowned bodies, and the rate was higher than that achieved with the digestive method. Moreover, TGGE is safer than the digestive method. Our study suggests that this method can aid diagnosis of drowning.