盐胁迫下白花前胡香豆素合成途径关键酶基因生物信息学分析

目的 系统研究白花前胡香豆素合成途径关键酶基因的蛋白结构特征,并明确关键酶基因在盐胁迫下的表达模式。方法 通过白花前胡前期转录组测序,确定前胡香豆素生物合成关键酶基因的序列,利用蛋白质结构分析预测软件对关键酶蛋白进行系统预测分析,并通过qRT-PCR分析盐胁迫下关键酶基因的变化规律。结果 关键酶基因编码蛋白都有着各自特殊的保守结构域和三级结构;0.15 mol/L NaCl处理4 h对PAL和4CL基因的表达水平影响最高,比对照组上调1倍;而COMT-S和GXM在不同处理时间下,基因表达水平均明显下降。结论 受盐胁迫后上调表达的香豆素合成途径关键酶基因,可以提高植物对逆境胁迫的耐受性。     

警察警务应激障碍及矫正

应激,是在应急事件中产生的高度紧张的情绪状态。人在应激状态,情绪反应特别迅速、猛烈,整个有机体的各部分发生显著变化,产生强烈的行为反应。超强度的应激,会对机体产生损害,降低完成任务的效率。警察在重大警务行动中,经常要面对突然发生的应急事件,这种应急事件导致超强度的应激情绪。警察如果应激能力不足,轻则会发生应激障碍,重则导致警务行动失败。     

Domestic Violence and Risk Factors among Korean Immigrant Women in the United States

Domestic Violence is a serious problem among Korean immigrant women in the United States. However, little is known about the incidence of domestic violence as well as risk factors predicting violence experienced in intimate relationships. The purpose of this study is to describe domestic violence among Korean immigrant women, including type and frequency of violence and predictive factors of domestic violence experienced by Korean immigrant women. One hundred and thirty-six Korean women completed questionnaires developed in this study. Results indicate that domestic violence is a major family problem for Korean immigrant women. Implications are discussed in terms of the need of social services for Korean immigrant women.     

简论宣传思想工作中的"十六"个要点

宣传思想工作是一项政策性很强的人的工作,也是我们党的优势和在新时期需要发扬光大的一项光荣传统。从目前的实践经验和结果看,在实际工作中,宣传思想工作应重点抓宣传、规划、投入,抓队伍、组织、领导等。     

中药提取物对应激小鼠肠道上皮内淋巴细胞和杯状细胞数量及IL-2水平的影响

为了观察中药提取物对应激小鼠肠黏膜屏障结构的影响,给10只C57BL/J健康应激小鼠连续灌服中药提取物,7d后光镜下观察小肠上皮内淋巴细胞(IEL)、杯状细胞(GC)的数量变化及测定肠黏膜IL-2的水平。结果显示,应激小鼠的肠黏膜IEL和GC的数量及肠黏膜IL-2水平下降;灌服中药提取物应激小鼠的肠黏膜IEL和GC的数量及肠黏膜IL-2水平与正常对照组无显著差异(P>0.05),而与未灌服中药提取物的应激小鼠相比,上述检测指标有显著性差异(P<0.05)。结果证实,该中药提取物在一定程度上可以增强应激小鼠小肠黏膜的屏障作用并提高小鼠免疫功能,增强抗应激能力。     

Determining Bruise Etiology in Muscle Tissue Using Finite Element Analysis

Bruising, the result of capillary failure, is a common physical exam finding due to blunt trauma and, depending on location and severity, a potential indicator of abuse. Despite its clinical relevance, few studies have investigated the etiology of capillary failure. The goal of this study was to determine whether capillaries primarily fail under shear stress or hydraulic‐induced tensile stress. An arteriole bifurcating into four capillaries was modeled using ANSYS 14.0 ^®. The capillaries were embedded in muscle tissue and a pressure of 20.4 kPa was applied. Any tensile stress exceeding 8.4 × 10⁴ Pa was considered failure. Results showed that failure occurred directly under the impact zone and where capillaries bifurcated, rather than along the line of greatest shear stress, indicating that internal tensile stress is likely the primary mode of capillary failure in bruising. These results are supported by the concept that bruising can occur via blunt trauma in which no shearing lacerations occur.     

Stress-related temporary hearing loss--evaluation of bio-humoral parameters: forensic and criminological applications

The body-alarm reaction results from the activation of hypothalamic-pituitary-adrenal axis, which can lead to physio-psychological phenomena such as an exclusion/occlusion of the sense of hearing. One hypothesis to explain this alteration consists in a hydromechanical dysfunction of the internal ear attributable to antidiuretic hormone. In this study, we evaluated the perception of acoustic stimuli administered in stressful conditions in 14 phobic patients and in 20 healthy subjects, in order to assess the influence of stress on perceiving capabilities. We also measured the concentration of salivary cortisol and IL-1β and neurovegetative parameters to objectivise and quantify the physiological reactions. Our results show a worse perception of the frequencies of the human voice under stress; these findings could have a dual value: in the legal field, concerning criminal liability, and on the operative context, regarding the efficiency of verbal communication among law enforcement officers in situations inducing intense emotional stress.     

芪红通络颗粒对大鼠脑缺血再灌注损伤保护作用及其机制研究

目的 观察芪红通络颗粒对大鼠脑缺血再灌注损伤的保护作用,并初步探讨其作用机制.方法 将SD大鼠随机分为假手术组,模型组,尼莫地平组,芪红通络颗粒高、中、低剂量(10.8、5.4、2.7 g/kg)组,采用线栓法建立大鼠局灶性脑缺血再灌注损伤模型.各组大鼠术前连续3d、术后连续2d灌胃给药,每日1次.术后72 h采用Longa法进行神经功能缺损评分,TTC染色法测定脑梗死体积,HE染色观察脑组织病理学改变,比色法测定脑组织丙二醛(malondialdehyde,MDA)含量和超氧化物歧化酶(superoxide dismutase,SOD)、谷胱甘肽过氧化物酶(glutathion peroxidase,GSH-Px)活力,Western blot法测定脑组织核因子E2相关因子2(nuclear factor erythroid-2 related factor 2,Nrf2)和血红素氧合酶-1(heme oxygenase 1,HO-1)蛋白表达水平.结果 与模型组比较,芪红通络颗粒高、中、低剂量组脑梗死体积显著缩小(P<0.05);高、中剂量组神经行为学障碍症状明显减轻(P<0.05),脑组织病理改变明显减轻;高、中、低剂量组脑组织中MDA含量明显降低,SOD和GSH-Px活力明显增加(P<0.05);高、中剂量组脑组织中Nrf2和HO-1蛋白表达水平明显升高(P<0.05).结论 芪红通络颗粒对大鼠脑缺血再灌注损伤具有较好的保护作用,其机制可能与其激活Nrf2/HO-1信号通路,抑制氧化应激反应,从而产生抗氧化作用有关.     

苓桂术甘汤含药血清对过氧化氢诱导的乳鼠原代心肌细胞氧化应激损伤及细胞凋亡的影响

目的 观察苓桂术甘汤含药血清对H2O2诱导的心肌细胞氧化应激损伤及细胞凋亡的影响。方法 建立H2O2诱导的心肌细胞氧化应激损伤模型,将心肌细胞随机分为正常对照组、H2O2模型组、空白大鼠血清（20%）组、苓桂术甘汤含药血清（5%、10%、20%）组,用苓桂术甘汤含药血清（5%、10%、20%）,分别预处理心肌细胞12 h后,加入100 μmol/L H2O2继续培养6 h,用MTT法测定心肌细胞存活率,比色法检测乳酸脱氢酶（lactate dehydrogenase,LDH）、超氧化物歧化酶（superoxide dismutase,SOD）、谷胱甘肽过氧化酶（glutathione peroxidase ,GSH- Px）活性及丙二醛（malonic dialdehyde,MDA）水平,用荧光核染色法观察细胞凋亡形态学变化,流式细胞术（Annexin Ⅴ- FITC/PI）检测细胞凋亡率,荧光探针法测定氧自由基水平。结果 与正常组比较,模型组心肌细胞活力明显降低（P<0.05）,氧自由基、LDH活性及MDA水平均显著升高（P<0.05）,SOD及GSH- Px活性均显著降低（P<0.05）,细胞核浓染数目增加,细胞凋亡率明显升高（P<0.05）;与模型组比较,苓桂术甘汤含药血清组心肌细胞活力明显升高（P<0.05）,GSH- Px、SOD活性均显著升高（P<0.05）,LDH活性,氧自由基、MDA水平均显著降低（P<0.05）,心肌细胞核浓染减少,细胞凋亡率明显降低（P<0.05）。结论 苓桂术甘汤能够清除细胞内氧自由基,抑制心肌细胞凋亡,增强细胞活性,对心肌细胞氧化应激损伤具有保护作用。