低氧和氧化应激中 NO介导培养神经元损伤机理的实验研究

目的探讨低氧、氧化应激中一氧化氮（NO）和氧自由基之间关系及其对培养神经元的损伤机理。方法对培养的新生大鼠神经细胞分别进行低氧、H2O2氧化应激处理和超氧化物歧化酶（SOD）抗氧化应激处理,用比色法等检测培养上清液中NO、丙二醛（MDA）、乳酸脱氢酶（LDH）和SOD含量变化指标。结果与对照组比较,低氧组和H2O2组的NO、LDH、MDA含量均显著增高,SOD含量显著降低,NO与SOD含量变化呈负相关关系。预先给予终浓度为200U/ml的SOD处理,可使神经细胞的NO、LDH和MDA释放量明显减少。各组间NO含量与LDH、MDA含量呈正相关关系。结论低氧、氧化应激促使神经元NO产生增多,NO有增加氧自由基对神经细胞的损伤作用。SOD具有清除氧自由基和减轻NO对神经元的损伤作用。     

功能性重组乳杆菌NC8-RLP对HUVEC细胞氧化应激损伤的抑制作用

为探究功能性重组乳杆菌NC8-RLP在缓解细胞内氧化应激损伤中的作用,首先构建氧化应激细胞模型,使用不同浓度的NC8-RLP作用细胞模型,通过MTT方法检测细胞的存活率和NC8-RLP毒性作用,观察细胞形态的变化;检测细胞模型内ROS含量的变化;采用Western-boltting检测NOX2、iNOS、Nrf2蛋白表达的情况。结果显示,成功建立氧化应激细胞模型OS-HUVEC,MTT结果发现NC8-RLP对HUVEC细胞无毒性作用,且具有一定的促进细胞增殖作用,同时可减少OS-HUVEC细胞凋亡。当NC8-RLP保护OS-HUVEC模型2 h后,细胞内的ROS含量降低,iNOS、NOX2蛋白表达量与阳性对照组相比明显减少,Nrf2蛋白表达量增加。说明NC8-RLP对缓解细胞内氧化应激损伤作用有很好的调控作用。     

Occupational stress,supervisor support,job satisfaction,and work-related burnout: perceptions of Turkish National Police (TNP) members

The present study examines whether, and to what degree, occupational stress in law enforcement is associated with job satisfaction, work-related burnout, and supervisor support. A total of 538 Turkish National Police (TNP) members form seven cities in Turkey completed the study survey. The results of the study indicate that the more TNP members experience their organization as stress inducing, the lower their job satisfaction levels, and the higher their burnout levels. Perceived operational stress was found to be significantly associated with their work-related burnout, but not with their job satisfaction. This study also suggests that there is an indirect causal effect of both organizational and operational stress on job satisfaction via supervisor support as mediator. Overall, the findings of this study illustrate a need for internal policy reform and managerial change in how the executives of TNP organize their agency and policies since organizational stressors are the most prevalent factors determining the work-related wellbeing of TNP members.     

柴地合方对慢性应激大鼠大脑前额皮质和海马单胺类神经递质的影响

目的：研究柴地合方对脑组织单胺递质含量的影响,探讨其抗抑郁作用可能的机制。方法：复制大鼠慢性应激抑郁模型,慢性给予柴地合方,采用高效液相色谱系统(HPLC)加电化学检测器(ECD)检测前额皮质和海马组织中单胺类神经递质及相关代谢产物的含量。结果：慢性应激大鼠前额皮质中去甲肾上腺素(NE)、5羟色胺(5—HT)和3,4—二羟基苯乙酸(DOPAC)含量显著降低,柴地合方可以翻转这种降低;慢性应激大鼠海马中NE、5—HT含量显著降低,柴地合方可以部分逆转降低水平。结论：柴地合方可升高慢性应激模型大鼠前额皮质和海马中NE和5—HT水平,这可能是其抗抑郁的机制之一。     

警察心理压力的产生及其处理方式

警察职业的特征决定了警察的心理压力很特殊,各级政府对警力的不当使用以及社会舆论也对警察的心理产生影响。本文试从这几个方面分析警察心理压力产生的根源及应对办法。     

高等职业院校教师职业压力辨析

高职院校教师职业压力是一个现实的问题。造成高等职业院校教师职业压力的原因是多方面的,缓解教师职业压力的方法也是多种多样的。作者认为,从高等职业教育定位角度寻求缓解教师的职业压力更为重要也更为准确。     

Detection of Malingered PTSD: An Overview of Clinical,Psychometric, and Physiological Assessment: Where Do We Stand?

ABSTRACT: Posttraumatic stress disorder (PTSD) is a condition that can be easily malingered for secondary gain. For this reason, it is important for physicians to understand the phenomenology of true PTSD and indicators that suggest an individual is malingering. This paper reviews the prevalence of PTSD for both the general population and for specific events, such as rape and terrorism, to familiarize evaluators with the frequency of its occurrence. The diagnostic criteria for PTSD, as well as potential ambiguities in the criteria, such as what constitutes an exposure to a traumatic event, are reviewed. Identified risk factors are reviewed as a potential way to help differentiate true cases of PTSD from malingered cases. The question of symptom overreporting as a feature of the disease versus a sign of malingering is discussed. We then examine how the clinician can use the clinical interview (e.g., SIRS, CAPS), psychometric testing, and the patient's physiological responses to detect malingering. Particular attention is paid to research on the MMPI and the subscales of infrequency (F), infrequency-psychopathology (Fp), and infrequency-posttraumatic stress disorder (Fptsd). Research and questions regarding the accuracy of self-report questionnaires, specifically the Mississippi Scale (MSS) and the Personality Assessment Inventory (PAI), are examined. Validity, usability, and cutoff values for other psychometric tests, checklists, and physiological tests are discussed. The review includes a case, which shows how an individual used symptom checklist information to malinger PTSD and the inconsistencies in his story that the evaluator detected. We conclude with a discussion regarding future diagnostic criteria and suggestions for research, including a systematic multifaceted approach to identify malingering.     

重复热应激致公兔性腺氧化损伤的研究

为探讨热应激对雄性兔性腺的氧化损伤机制,以体重相近的110日龄雄性新西兰兔为研究对象,每天在固定时间分别以31～34℃和38～40℃进行热应激,并分别在热应激后的第15、30、45及60天处死兔,取样检测性腺组织内总抗氧化酶活性、丙二醛及蛋白质羰基含量。结果显示,随着温度升高,睾丸和附睾组织丙二醛含量增加,总抗氧化酶活性降低,蛋白质羰基含量呈上升趋势,表现出明显的温度-时间效应。表明高热可改变兔睾丸和附睾的正常生理机能,使自由基在组织内累积,引起蛋白质氧化损伤,进而降低繁殖机能。     

Witnessing an Accidental Shooting at the Police Training Academy

Abstract

After an accidental shooting at a police training academy, outcomes of the class involved (n = 45) were compared with those of several comparison classes (n = 154) immediately and one year later. Results revealed a modest impact, but an increase in PTSD symptomology was observed from academy training to the one-year follow-up. Recruits in the affected class demonstrated more prejudicial attitudes toward women in law enforcement, perhaps because the shooter was a woman. The findings suggest that mistakes made by women within law enforcement may be generalized as stereotypes, and that further research is needed on the psychological impact of becoming a police officer.     

Posttraumatic stress disorder symptom severity and fear of personal crime: exploring their interrelationship as a function of risk estimation

Both trauma psychology and criminology have studied the psychological correlates of crime victimization. While the former discipline has primarily focused on the development of posttraumatic stress disorder (PTSD) among crime victims, the latter has particularly studied the association between history of victimization and fear of crime. A major difference between both concepts is that PTSD is experienced in relation to previous victimization, while fear of crime does not necessarily follow from previous victimization and is primarily experienced in anticipation of possible future victimization. Despite their different orientations, both perspectives share one central tenet: they both argue that feelings of anxiety are accompanied by increased perceptions of risk for future victimization. Given this theoretical overlap, both types of anxiety may correlate with each other. The current study explored this topic in a sample of Dutch university students (N = 375) and found that PTSD symptom severity and fear of crime were significantly associated with each other, both in univariate and multivariate analyses. This association was stronger for participants who scored higher on perceived risk of personal crime victimization than for those who scored lower. Results were discussed in light of study limitations and directions for future research.