褪黑激素对犬脂肪干细胞向雪旺细胞分化过程中凋亡发生的抑制作用研究

为了探讨褪黑激素(Mel)对犬脂肪干细胞(ADSCs)向雪旺细胞(SCs)诱导分化过程中凋亡发生的影响。本实验将犬ADSCs诱导分化为SCs,并使用免疫荧光法进行鉴定。采用CCK8法选取Mel的最佳浓度。应用q RT-PCR法和Western-blot法检测无Mel组和Mel处理组中ADSCs向SCs诱导过程中细胞凋亡相关基因Caspase-3、Caspase-8、Bcl-2和Bax的m RNA和蛋白表达水平。结果显示,50 nmol/L Mel处理过的细胞活力最高;ADSCs向SCs诱导后S-100和GFAP呈阳性表达,并且观察到Mel的添加并不会影响到SCs的表型特征。ADSCs向SCs诱导后Bcl-2表达量明显低于对照组,Caspase-3、Caspase-8和Bax的表达量显著高于对照组,细胞凋亡较多;在加入Mel后Bcl-2表达量显著升高,Caspase-3、Caspase-8和Bax的表达量显著下降。结果说明Mel可以显著抑制犬ADSCs向SCs诱导分化过程中的凋亡发生。     

An Unusual Cause of Headache and Sudden Death of a Young Sailor—Postmortem Computed Tomography and Histological Findings of a Fatal Retroperitoneal Malignant Mixed Germ Cell Tumor

A 26‐year‐old Caucasian sailor, with no past medical history aside from headache for the last 1 week, was found dead in his cabin. The body was stored in a refrigerator on board and disembarked for autopsy 3 days later. Autopsy showed a large, nodular, necrotic and hemorrhagic retroperitoneal mass, and smaller hemorrhagic nodules in the brain, lungs, liver, and left kidney, with the brain being markedly edematous. Both testes were descended and normal. Histologically, the retroperitoneal mass showed a malignant mixed germ cell tumor comprising choriocarcinoma, embryonal carcinoma, and teratoma components. Retroperitoneal extragonadal germ cell tumors are uncommon, and this case of a young male who presented with headache and sudden death due to metastases is extremely rare.     

精子细胞定向捕获与分离技术初步研究

目的探索并研究精子细胞定向捕获与分离技术,初步建立精子细胞特异性分离与DNA提取的方法与试剂体系。方法通过特异性定向捕获复合体（精子特异性抗体一磁性纳米微球）的制备,在一定的试剂体系环境下,实现精子细胞的定向捕获与分离。结果能够实现精子细胞的定向富集与分离,通过后续的提取过程,获得了高质量的DNA,并获得了相应的完整STR分型结果。     

混合斑中精子细胞分离及其DNA制备方法

目的尝试建立一种检测混合斑中精子细胞的方法。方法使用显微操作法捕获精子细胞,全基因组扩增(多重置换扩增)精子细胞DNA。结果对10管精斑检材的全基因组扩增,获得了高产、保真的产物。使用50μL体系对20个精子细胞直接进行全基因组扩增,省去了对起始模板的纯化过程,DNA扩增倍数达30000倍以上,片段长度大多在15 kb以上,其STRs复合扩增分型结果有可参照性。结论显微操作法可以有效捕获精子细胞,排除干扰,多重置换扩增可以提供足够量的产物用于法医DNA分析,该方法具有可行性。     

损伤皮肤中组胺荧光显微定位定量与损伤时间推断的实验研究

本文应用显微荧光分光光度计法研究大白鼠皮肤创缘组织中组胺的分布和含量,并用甲苯胺蓝法观察创缘肥大细胞形态和数量的变化。结果发现,创缘真皮乳头层出现扩散的细胞外黄色荧光和肥大细胞脱颗粒现象为生前伤的重要特点;组胺荧光分布范围及增多的程度与损伤时间密切相关。从而为损伤时间推断提供了一种新的方法。     

The probability of achieving full allelic representation for LCN-STR profiling of haploid cells

Modern forensic techniques allow DNA to be extracted from ever decreasing amounts of cellular material. Low copy number (LCN) profiling enables the production of STR profiles from small numbers of cells. Moreover, methods such as laser micro-dissection enables forensic scientists to potentially isolate individual cells for PCR. The DNA derived from haploid cells (semen) is a common source of forensic evidence in sexual assault cases. Haploid cells contain only half the DNA complement of diploid cells (3 pg compared to 6 pg). The smaller the number of cells sampled, the smaller the probability that there is a full representation of the alleles comprising the donor profile. This paper investigates the relationship between the number of cells sampled and the probability of full representation of all alleles in the donor sample. It also considers the effect of typing several loci as opposed to just a single locus.     

生脉成骨胶囊对激素性股骨头坏死髋周间充质细胞增殖的影响

目的 观察生脉成骨胶囊对激素性股骨头缺血性坏死大鼠股骨和髂骨间充质干细胞增殖及成骨细胞矿化结节生成的作用.方法 将60只SD雄性大鼠随机分为6组:空白对照组、模型组、生脉成骨预防组、阿仑磷酸钠治疗组、丹郁骨康治疗组、生脉成骨治疗组,通过测定骨髓间充质细胞的增殖能力、成骨细胞矿化结节数,观察生脉成骨胶囊对股骨和髂骨近端...     

干细胞的新来源——羊膜

为了给羊膜干细胞深入研究提供借鉴,介绍了羊膜的组织特点,综述了羊膜上皮干细胞及羊膜间充质干细胞的生物学特性及其诱导的潜能和鉴定方法,并对其在临床医学应用的前景进行了展望。提出羊膜组织来源的干细胞为干细胞的研究提供了新来源、新思路。     

车辆内接触DNA检测方法的分析比较

目的探讨汽车内接触DNA的分离方法及遗传标记分型效率。方法收集单人长期驾驶的11辆小型轿车,采用粘取法和擦拭法富集方向盘、变速杆和手刹三个部位的脱落细胞,采用磁珠法和硅胶膜法提取基因组DNA,采用GoldenEye^TM 20A和PowerPlex■Fusion进行扩增,并对检验结果进行比较分析。结果方向盘在基因座分型正确率、等位基因drop-in和drop-out基因座比率、单基因座正确率以及单基因座等位基因drop-in和drop-out率六个方面均表现最好,其次为变速杆,最差为手刹;擦拭法和粘取法之间DNA提取在获得的DNA总量和STR检测正确成功率方面无统计学差异;PPFusion与20A的总体基因座分型比较正确率无差异,但单基因座正确率优于20A,drop-out发生率低于20A,drop-in发生率高于20A。结论汽车内脱落细胞的检测可优先采集方向盘部位,根据载体质地选择擦拭法或粘取法采集脱落细胞,选用硅胶膜法或磁珠法提取DNA,PPFusion和20A两个试剂盒均可,分析结果时需特别注意drop-in和drop-out。