木瓜齐墩果酸和熊果酸等三萜酸类薄层色谱分离鉴别

［摘要］目的 建立木瓜齐墩果酸、熊果酸等三萜酸薄层色谱分离鉴别方法。方法 将点于硅胶G板上的样〖JP2〗品点浸入1%碘 二氯甲烷溶液中预处理,以环己烷-丙酮-乙酸乙酯-甲酸(9∶2∶1∶0.2)为展开剂。结果 木瓜中熊果酸、齐墩果酸以及3-O-乙酰熊果酸能够被很好地分离和检识。结论薄层色谱法简便、可靠,使木瓜鉴别更具客观性和专属性。     

关于《财政与金融教程》的教学思考

在财政与金融课程的教学中,要兼顾财政与金融二者的基本知识和基本理论,领会教学目的要求,把握教材结构体系;要根据教材的重点,运用科学的方法,做到理论联系实际,结合成人教育的特点尝试案例教学,学以致用.     

The use of crossover immunoelectrophoresis to detect human blood protein in soil from an ambush scene in Kosovo

This study examines the survivability of human blood proteins in soils from a year and a half old ambush scene in Kosovo. A total of 72 soil samples were collected, a number of which were directly associated with bone fragments or bullet projectiles. The samples were examined using crossover immunoelectrophoresis (CIEP) to determine the presence of blood protein and species affiliation. Human blood proteins were identified in 44 of the 72 samples (61%) with the majority of the positive observations (29 of 44) found 0.0-4.5 cm below ground surface (65%). Chi-squared and two-sample difference of proportions tests confirmed significant differences between samples with and without associated physical evidence and the presence and depth of human blood proteins. While DNA has largely replaced immunological analysis in forensic analyses, our results suggest that in particular situations, CIEP may still be a valuable tool in criminology.     

国产试剂盒Goldeneye~(TM)20A在亲权鉴定中的应用评估

目的评估GoldeneyeTM20A试剂盒在亲权鉴定中的应用价值。方法应用GoldeneyeTM20A试剂盒对289宗亲权鉴定案例中的FTA卡血样本基因组DNA进行PCR扩增,扩增产物用ABI 3130xl遗传分析仪进行毛细管电泳,GeneMapper v3.2和GeneMarker HID软件进行基因分型及统计学分析,并与IdentifilerTM、SinofilerTM、PowerPlex16 3种试剂盒进行比较。结果采用GoldeneyeTM20A试剂盒,累积非父排除概率(CPE)为0.999 999 996,累积个人识别能力(CPD)达0.999 999 999 999 999 999 999 932 44,与目前常用的3种试剂盒相比较,GoldeneyeTM20A试剂盒在不排除的案例中具有更高的CPI值;在排除的案例中具有更多的排除指标。结论国产GoldeneyeTM20A试剂盒在亲权鉴定中有较高的应用价值。     

我国数字动画产业发展思路分析

数字动画的发展对经济产生着越来越大的影响,数字动画产业已经成为未来最有潜力的行业之一.我国数字动画产业要走独立发展的路子,注意政策的配套及各部门间的配合.     

基于特征轮廓线的三维颅骨配准方法

目的针对三维颅骨模型在初始姿态和分辨率上差异较大的情况,提出一种基于特征轮廓线的颅骨配准方法。方法首先,提取三维颅骨模型的眼眶、鼻框、颞骨、上颌骨以及下颌骨等轮廓线;然后,根据轮廓线的长度以及轮廓间的最短距离判断轮廓线的类型,并建立两个待配准颅骨在轮廓线上的对应关系,从而实现颅骨的粗配准;最后,通过添加权值的方式来改进迭代最近点(iterative closest point,ICP)算法,并采用该改进的ICP算法实现颅骨的细配准,从而达到两个颅骨精确配准的目的。结果通过将一个未知颅骨与颅骨数据库中的300个颅骨的配准实验表明,提出的基于特征轮廓线的配准算法可以实现三维颅骨模型的精确配准,并在细配准阶段取得了较高的配准精度和收敛速度。结论基于特征轮廓线的配准算法是一种快速、高精度的三维颅骨模型配准方法。     

Testing Reliability of the Computational Age‐At‐Death Estimation Methods between Five Observers Using Three‐Dimensional Image Data of the Pubic Symphysis,

In an effort to standardize data collection and analysis in age estimation, a series of computational methods utilizing high‐dimensional image data of the age indicator have recently been proposed as an alternative to subjective visual, trait‐to‐phase matching techniques. To systematically quantify the reproducibility of such methods, we investigate the intrascan variability and within‐ and between‐observer reliability in initial scan data capturing and editing using 3D laser scans of the Suchey–Brooks pubic symphysis casts and five shape‐based computational methods. Our results show that (i) five observers with various training background and experience levels edited the scans consistently for all three trials and the derived shape measures and age estimates were in excellent agreement among observers, and (ii) the computational methods are robust to a measured degree of scan trimming error. This study supports the application of computational methods to 3D laser scanned images for reliable age‐at‐death estimation, with reduced subjectivity.     

Comparison of Catalytic and Immunological Amylase Tests for Identifying of Saliva from Degraded Samples

The stability of salivary α‐amylase is a critical factor in both catalytic and immunological method‐based forensic saliva identification. This study aimed to assess the sensitivity of catalytic and immunological tests on degraded saliva samples. Degraded saliva stains were prepared by microbial decomposition using humid soil. Salivary α‐amylase activity was catalytically detected both qualitatively and quantitatively using the Phadebas^® amylase test. As immunological methods, we conducted qualitative and quantitative tests using the RSID?‐saliva test and ELISA, respectively. Salivary α‐amylase activity of degraded samples (incubated at 37°C for 12 h) was significantly lower than that of controls in the quantitative tests. All the degraded samples obtained by the humid soil produced negative results in the Phadebas^® tests, but showed positive results in the RSID?‐saliva test and ELISA. These results suggest that immunological tests are effective for testing degraded saliva samples that have lost their enzymatic activity.     

Application of 3D Imaging Technology to Latent Fingermark Aging Studies

In most latent fingermark aging studies, two‐dimensional (2D) features are obtained from photo images, scans, or inked impressions. However, some relevant information is possibly being missed because fingermarks are three‐dimensional (3D) objects that age in all three dimensions. A feature that has not been carefully examined is how the height of ridges changes over time. In this report, a 3D imaging technology—called optical profilometry—is introduced as a tool for the visual examination of the aging process. Optical profilometry is a nondestructive technology that allows the visualization and data acquisition of unprocessed latent fingermarks. Detailed ridge images and spatiotemporal data were successively obtained on the x‐, y‐ and z‐axis, delivering 3D topographical information. OP was able to detect the loss of ridge heights over time. The feasibility of employing this technology to collect data on the aging process of ridges has been proven.     

10+3框架下的东北亚区域合作--兼谈对东北亚合作的几点新认识

10+3框架的产生,使东北亚次区域合作有了制度上的保障,以中、日、韩为核心的东北亚合作成了东亚区域合作的一个重要组成部分。当前,中、日、韩合作的最大障碍是中日之间缺乏互信,双方应当“以史为鉴,面向未来”,共同推动东北亚合作。东北亚合作应当坚持开放性,以 10+3为主,多渠道并进。