Revived and Discouraged: Evaluating Employment Barriers for Section 3 Residents With Criminal Records

Section 3 was established in the Housing and Urban Development (HUD) Act of 1968 to provide employment for public housing residents in distressed communities while rebuilding underserved neighborhoods. As a provision that recipients of HUD funding must comply with, Section 3 reporting agencies are having trouble securing employment for ex-offenders. This is problematic since low-income ex-offenders unable to secure stable employment are more likely to recidivate. Research evaluating the specific barriers to employment for Section 3 residents with criminal records and policy recommendations are sparse although the problem is prevalent in communities nationwide. This study uses San Antonio, Texas as an example for conducting a policy review to identify the barriers to employment for Section 3 ex-offenders. The results of the qualitative analysis indicate that at the national level, HUD and the Section 3 provision do not create barriers to employment but state and local policies and practices do.     

滋阴潜阳方对高血压大鼠淋巴细胞内PKA蛋白表达和cAMP水平的影响

目的:探讨高血压的神经免疫调节机制和滋阴潜阳方对高血压大鼠淋巴细胞内信号转导作用的可能环节.方法:采用免疫组织化学染色法和DP801图像分析系统检测"二肾一夹"高血压大鼠淋巴细胞内蛋白激酶A(PKA)蛋白表达.采用放射免疫法检测环磷酸腺苷(cAMP)水平的改变.结果:模型组大鼠淋巴细胞内PKA蛋白表达和cAMP水平明显增强,滋阴潜阳组对此有抑制作用.结论:滋阴潜阳方抑制淋巴细胞内PKA蛋白表达和cAMP水平可能是其发挥神经免疫调节作用的机制之一.     

Alternative direct-to-amplification cell lysis techniques for forensically relevant non-sperm cells

While efforts have been made to reduce the pervasive backlog of sexual assault evidence collection kits, the actual laboratory process remains very time-consuming due to the requirement of a differential lysis step before DNA purification, as well as intricate mixture analysis towards the end of the DNA workflow. Recently, an alternative, direct-to-amplification sperm lysis method (using 1 M NaOH) was identified. However, a direct cell lysis method for non-sperm cells has not been identified yet. Thus, the primary objective of this work was to find an alternative method that is quick, inexpensive, and does not require multiple purification steps for the lysis of non-sperm cells in sexual assault samples. In this study, vaginal swab samples were lysed with the control method, prepGEM™, as well as six alternative reagents: alkaline buffer with 25–200 mM NaOH, high-salt stain extraction buffer, modified radioimmunoprecipitation assay (RIPA) buffer, mammalian protein extraction reagent (M-PER™), digitonin buffer, and urea/thiourea buffer. Quantification using Quantifiler® Trio of vaginal and semen lysates revealed that the alkaline (25 mM NaOH) and M-PER™ methods were efficient for the lysis of vaginal epithelial cells without substantial sperm cell lysis. Following quantification, analysis of STR profiles from vaginal lysates revealed that the M-PER™ method showed promising results across all metrics examined, including the percentage of detected STR alleles, mean peak heights, peak height ratio, and interlocus balance. Thus, this method was recommended as an alternative to the traditional differential lysis method for non-sperm cells given its ability to produce amplification-ready lysates without any DNA purification step.     

浅谈数据挖掘技术在公安领域中的应用

当前,我国各类公安信息化系统在警务数据处理与查询方面发挥了重要作用,但信息的深层次挖掘很不充分,分析功能相当欠缺。通过决策树在犯罪风险模型和关联规则在公安出入境数据的挖掘,发现、提取出有价值的知识和规则,为辅助决策和参考提供科学依据。     

毛泽东统筹兼顾、协调发展思想再探讨——兼论构建和谐社会的方法论

我国社会主义建设中统筹兼顾、协调发展思想的倡导执行始于第一代党的领导集体,其中毛泽东的理论成就和历史贡献最大。本文集中阐述了毛泽东探索社会主义现代化道路中统筹兼顾、协调发展的主要思想成果,以及为我们今天构建社会主义和谐社会所提供的方法论启示。     

10+3框架下的东北亚区域合作--兼谈对东北亚合作的几点新认识

10+3框架的产生,使东北亚次区域合作有了制度上的保障,以中、日、韩为核心的东北亚合作成了东亚区域合作的一个重要组成部分。当前,中、日、韩合作的最大障碍是中日之间缺乏互信,双方应当“以史为鉴,面向未来”,共同推动东北亚合作。东北亚合作应当坚持开放性,以 10+3为主,多渠道并进。     

金融机构并购的法律支持路径

并购是当前解决我国金融机构市场退出难题的最为实际有效的办法。然而好的制度设计才能使金融机构的并购真正富有成效,金融机构并购的相关立法必须体现谁受益谁承担成本的原则,只有真正减少行政干预,让金融机构立足市场,引入民营金融机构参与竞争,并尽早出台针对金融机构切实可行的并购实施细则,金融领域的市场退出机制方能得以建立。     

我国数字动画产业发展思路分析

数字动画的发展对经济产生着越来越大的影响,数字动画产业已经成为未来最有潜力的行业之一.我国数字动画产业要走独立发展的路子,注意政策的配套及各部门间的配合.     

关于《财政与金融教程》的教学思考

在财政与金融课程的教学中,要兼顾财政与金融二者的基本知识和基本理论,领会教学目的要求,把握教材结构体系;要根据教材的重点,运用科学的方法,做到理论联系实际,结合成人教育的特点尝试案例教学,学以致用.     

口蹄疫病毒3A蛋白和FLAG标签的融合表达与血清学分析

通过对比各短肽标签,选择由8个氨基酸组成的FLAG标签弥补口蹄疫疫苗毒株OZK 3A蛋白的93～102位10个氨基酸缺失,原核表达FLAG与3A的嵌合蛋白,并命名为3AF。同时也表达了OZK毒株原始3A蛋白。分别以3A和3AF蛋白作为免疫原免疫6～8周龄雌性BALB/c小鼠,采用ELISA和Western-blot分析融合蛋白的血清学反应活性。结果表明,3A蛋白和3AF蛋白均能在大肠杆菌中有效表达,且主要以可溶性形式表达。纯化的3AF蛋白能与FLAG单抗特异性反应而未能诱导免疫小鼠产生针对该标签的抗体,体现了体外和体内的生物反应性差异。FLAG标签对3A蛋白的表达水平、表达形式等未产生显著影响。本研究为下一步FLAG标记病毒的拯救及其应用奠定了基础。