SYBR GreenI实时荧光PCR技术在人类DNA定量中的应用

目的建立SYBR Green I实时荧光定量PCR技术在法医物证检验中的应用。方法应用SYBR Green I实时荧光定量PCR技术对各种生物检材进行定量分析,并在此基础上进一步分析STR。结果得到了实验的各种生物检材的准确定量。结论SYBRSYBR Green I实时荧光定量PCR技术是一种高效且廉价的检测基因拷贝数的方法,具有法医学应用价值。     

线粒体DNA nt8584、nt8701单核苷酸多态性研究

目的建立一种快速、准确的线粒体DNA单核苷酸多态性位点检测方法。方法收集62份无关个体血液样本,应用荧光定量PCR技术研究线粒体DNA nt8584、nt8701位点在中国汉族人群中的多态性。结果 nt8584位点检测到10例nt8584A、52例nt8584G,nt8584A/G变异频率为16∶84;nt8701位点检测到27例nt8701A、35例nt8701G,nt8701A/G变异频率为44∶56。结论所建立的荧光定量PCR方法分型准确、耗时短,适用于法医DNA检验。     

实时荧光定量PCR检测硅藻UPA基因在溺死诊断中的研究

目的建立硅藻UPA条形码基因的实时荧光定量PCR检测方法,探讨其在溺死诊断中的应用价值。方法对Gene Bank中登录的硅藻UPA基因序列进行比对获得同源序列,据其设计硅藻门通用引物。对2种人体常见共生菌(大肠杆菌、双歧杆菌)、3种浮游细菌、15种浮游藻类、32具尸体(其中生前溺水死亡28例,死后抛尸1例,陆地死亡3例)的组织样本(肺、肝、肾)及尸体发现地水样提取DNA,采用设计的引物进行特异性、灵敏度、重复性试验,分析检材中硅藻检出情况,统计硅藻阳性率,以上述组织样本的微波消解-真空抽滤-自动扫描电镜法检测结果对建立的q PCR检测方法进行比较评估,比较该方法与PCR-毛细管电泳检测法的灵敏度。结果引物UPA99仅对硅藻标准株(针杆藻、舟形藻、直链藻、小环藻、菱形藻)DNA具有扩增,扩增产物熔解曲线平稳,峰尖且窄,熔解温度为(87±1)℃。以p MD18-T重组质粒为标准品,检测区间为1.56×10~2-1.56×10^-5ng/μL,建立实时q PCR方法的灵敏度为1.56×10^-5ng/μL,而PCR-CE方法的灵敏度为1.56×10-3ng/μL。批间及批内变异系数均低于2%,具有较高的重复性和稳定性。对于生前溺水尸体肺、肝、肾的检出率依次为89.3%,71.4%,64.3%。结论基于硅藻UPA基因设计通用引物,建立的q PCR硅藻检验法特异性高、灵敏度高、重复性好,应用于溺死诊断具有较好的应用前景。     

Use of a commercially available hydrogel as a novel DNA surface sampling tool

A common requirement in the military, law enforcement, and forensic mission space is the need to collect trace samples from surfaces using a method that not only readily captures the sample but also retains its integrity for downstream identification and characterization. Additionally, collecting samples from three-dimensional objects (e.g., shell casings) is a challenge for which there is currently no validated standardized approach. Recently, hydrogels have been shown to have the potential for surface collection of trace bacterial spores, amino acids, and DNA. To test whether these hydrogels can serve as a viable collection medium for sampling DNA from surfaces, we carried out a series of preliminary tests examining collection efficiency and suitability of hydrogel material to recover samples of diluted, dried human DNA on a smooth polycarbonate surface. The recovery of surface DNA using a commercially available hydrogel was examined, and the efficiency compared to samples collected using a standard foam collection swab. DNA collected using the hydrogel and swab methods was then examined using quantitative polymerase chain reaction (qPCR) and short tandem repeat (STR) analysis to determine whether the collection material was compatible with these downstream processes. The hydrogel material used for this study collected the experimental DNA with comparable efficiency to standard collection swabs. In addition, qPCR and STR analyses demonstrated compatibility with the hydrogel collection and extraction process. These data suggest that hydrogels have the potential to be used as sample collection materials and deserve further characterization to elucidate their utility in collection from irregularly shaped, three-dimensional surfaces/materials.     

Are there really two cultures? A pilot study on the application of qualitative and quantitative methods in political science

In their 2012 publication A Tale of Two Cultures, Gary Goertz and James Mahoney argue that empirical research in the social sciences aiming at causal inference can be differentiated into a qualitative and a quantitative methodological culture. The two cultures differ fundamentally in how researchers approach and implement empirical studies. The argument is well laid out and comprehensively illustrated, but the empirical validity of the two cultures hypothesis has not yet been evaluated systematically. This note introduces a research project that aims to test the two cultures hypothesis via an empirical analysis of how qualitative and quantitative methods are applied. To determine whether there is a qualitative and quantitative method culture, the researchers initially sampled 30 articles from three journals (Comparative Political Studies, European Journal of Political Research, World Politics) in the 2008–2012 period. Based on this dataset, no evidence was found for the existence of coherent systems of methods practices in political science.     

Time‐dependent Expression of MMP‐2 and TIMP‐2 after Rats Skeletal Muscle Contusion and Their Application to Determine Wound Age

The ability to determine vitality and estimate the survival period after a wound is critical in routine forensic practice. The mRNA levels of MMP‐2 and TIMP‐2 were examined using quantitative real‐time RT‐PCR to determine the age of a wound. Furthermore, the colocalization of them with Macrophage Marker, respectively, was detected by double immunofluorescence, and a standardized rat model of skeletal muscle contusion was established. In the antemortem contused groups, a large number of macrophages showed positive staining for MMP‐2 and TIMP‐2, and the expression of MMP‐2 and TIMP‐2 mRNA increased sharply at 3 days postinjury, with relative quantities of 5.75 and 2.98. No samples in the other groups showed relative quantities of >5.75 and 2.98; therefore, relative quantities exceeding 5.75 and 2.98 were strongly indicated 3 days after contusion. In addition, there was a significant decrease in the relative quantity in the postmortem contused groups, indicating that they were useful for diagnosing vitality.     

Re-packaging old policies? ‘Abenomics’ and the lack of an alternative growth model for Japan's political economy

Abstract

‘Abenomics’ has continued to attract the attention of both the national and international media and a broad range of scholars. There are different and contested views of Abenomics and its impact upon the Japanese economy. This article argues that those more Keynesian-style remedies that form part of Abenomics have not been able to address Japan's longer-term problem of weak demand, especially in terms of private consumption. This is in large part due to the liberalising measures that also form part of Abenomics, and which are incompatible with the Keynesian remedies pursued. Whilst Abenomics has the potential (at least in the short-to-medium term) to improve the profitability of Japanese businesses, in the absence of a corresponding move to redistribute corporate wealth to labour, Abenomics also represents a hazard to future economic growth in Japan.     

Restoring Choice: The Relationship Between Offense Seriousness,Intervening Time,and Victims’ Responses to the Offer of Restorative Interventions

This study investigates victims’ responses to the offer of involvement in a restorative intervention. It tests the hypotheses that victims’ choices are related to the seriousness of the offense (H1), and that this relationship is moderated by how long after the offense the restorative intervention is offered (H2). A cross-sectional, between-subjects design was used, drawing on data from 256 offenses collected for operational purposes by a restorative justice service. Victims of medium-seriousness offenses were most likely to choose indirect and direct reparation. Intervening time moderated the relationship between seriousness and victim choice: victims of low-seriousness offenses became more likely to choose community reparation with time, victims of medium-seriousness offenses became less likely to choose direct or indirect reparation, and there was no change with time for victims of the most serious offenses. These results suggest nonlinear relationships between seriousness, intervening time, and victim choice. Although more complex and in different directions than anticipated, they lend some support to both hypotheses. The study highlights qualitative differences between types of restorative interventions, points to a significant disparity between victims’ choices and processes the literature suggests are of most benefit, and raises questions regarding when and how restorative interventions should be offered.     

云南省生育率下降与经济增长的定量研究

应用新经济增长理论的方法,在相应假设条件下,通过设立动态人口-经济运行模型,从人口作为消费者影响资本积累和作为劳动者影响生产两方面入手,借助人口增长的模拟,可以定量分析出云南省人口控制下生育率迅速下降对云南省经济增长和人民生活提高的贡献.