IgE死后生化学检测的研究现状及应用展望

过敏性休克导致死亡的案例在法医学实践中常有发生,然而由于缺少特异的病理形态学指征,导致过敏性休克死亡的法医学鉴定面临一定的困难。IgE作为介导I型超敏反应的关键介质,其死后生化学检测对过敏性休克死亡案例的法医学鉴定具有辅助作用。但在实际应用中,血液等检材常受溶血等死后变化因素影响,尤其我国尸体保存以冷冻为主,溶血程度更加严重,对IgE等生化学指标的检测造成极大干扰。本文就目前IgE死后生化学检测的研究现状、生物学检材的选取及检测方法进行综述,并对现实应用中所面临的问题进行探讨。     

Volatile Organic Compounds,Spectral Characterization and Morphology of Ammonium Nitrate Fuel Oil (ANFO) Samples

Ammonium nitrate fuel oil is an explosive mixture found in most antipersonnel landmines (APL) buried throughout the Colombian territory. During more than 50 years of internal conflict, the Colombian government has found that trained dogs are the most effective method to detect APL. However, the olfactive signature in ANFO is unknown and also if there are differences in detection related to the explosive manufacturing origin. Therefore, this work begins with the analytical validation of the method used to determine ammonia, in its derivatized form as carbamate, released by home-made ANFO using HS-SPME-GC-FID. Once validated, the method was used to identify ammonia and other organic volatile compounds present in ANFO, under laboratory and simulated field conditions. The validation process includes the evaluation of the optimum conditions for the derivation and extraction of butylcarbamate, the determination of the working ranges with linear response in FID, the limits of detection and quantification, the sensitivity, and the precision. The results of the validation established linearity and sensitivity in a concentration between 20 and 120 mg/L, as well as low limits of detection and quantification of 6.4 and 21.4 mg/L, respectively. Also, an intermediate precision of 11% for butylcarbamate with a repeatability of 8%. The validated method showed in real samples of home-made ANFO besides ammonia, the presence of low molecular methylamines, and also exhibited differences in volatile compositions according to the origin. The objective of this work is to offer a reliable analytical methodology for the extraction and analysis of volatile compounds from ANFO.     

论闭路电视监控系统信息资料在侦查中的运用

近年来,随着我国经济的飞速发展以及人民群众防范意识的不断提高,闭路电视监控系统越来越普及,并得到了广泛运用。为了使闭路电视监控系统信息资料能够更好地运用到侦查破案中去,文章从闭路电视监控系统信息资料概述、信息资料在侦查破案中的运用、如何提高信息资料的使用效率以及使用中应注意的问题四个方面进行了论述,并提出了运用闭路电视监控系统信息资料的新理念、新方法。     

Stage model for female criminals: business school students’ perceptions of white-collar offenders

We know that half of the population in Norway is female, and we know that females represent 6% of the white-collar crime prison population. In the stage model overview, we derive percentages from the literature into the gender model to explain stepwise reduction from 50% to 6%. In our empirical research, we asked two groups of business school students to come up with their own estimates for the stages in the model for female criminals. While estimates from executive students resulted in 3% women in prison, bachelor students’ estimates resulted in 10% women in prison. The most obvious discrepancy between the research literature and our two survey groups is related to relative convictions. Based on the literature, we suggested that female defendants receive more serious convictions because they may perceive and feel more guilt for a crime, for example in terms of regret, shame and depression. Thus women may have a tendency to confess more easily. Both executive students and bachelor students disagree with this estimate of 140%, as they suggest 62% and 69% respectively. One reason for their suggestion of less serious convictions for female white-collar criminals – sometimes labelled pink-collar criminals – might be that family situation and other elements are taken into account before a verdict is passed on a woman. Another substantial discrepancy is related to detection risk. The literature suggests a low detection risk for women, but may be not as low as we estimated at 30%. Both executive and bachelor students believe that the gender difference in detection likelihood is not that formidable, as they suggest 75% and 65% respectively.     

Trading in development: norms and institutions in the making/unmaking of European Union–African,Caribbean and Pacific trade and development cooperation

This paper offers a contribution to recent debates on European Union (EU) external trade and development policy, with a specific focus on the African, Caribbean and Pacific (ACP) group of countries. The question asked is why the EU encountered such difficulties in the attempt to translate its normative preferences for freer trade and closer economic integration into a series of binding agreements? Drawing on both economic constructivist and historical institutionalist insights, it is argued that the case for reform initially rested on a strong convergence between institutions and ideas, enabling the EU to discursively present desired policy reforms as necessary to satisfy World Trade Organisation trade rules. However, in due course, the institutional dynamics behind the latter began to diverge from the EU's policy preferences and blunt its norm-based argument – thus creating the space for transnational coalitions to, first, question and, ultimately, undermine aspects the EU's trade and development prospectus for the ACP.     

病毒性出血败血症病毒实时荧光RT-RPA恒温检测方法的建立

为建立一种简单、快速的病毒性出血败血症病毒(VHSV)检测方法,本研究通过比较分析VHSV N基因的保守序列,设计了多对引物和一条探针。经过筛选优化,建立了实时荧光RT-RPA检测方法。该方法在39℃恒温反应20 min即可快速、特异性地检测出VHSV,与其他水生动物病毒不发生交叉反应,该方法检测限为1.83×10^3PFU/mL,利用本研究方法对30份鲑鳟鱼临床样品以及9份鱼类疫病病原样品进行检测,检测结果与荧光定量PCR方法结果一致。上述结果表明,本研究建立的检测方法操作简单,反应灵敏,结果可靠,仪器依赖性低,适用于现场对VHSV快速检测。     

副猪嗜血杆菌抗体间接血凝检测方法的建立及应用

将副猪嗜血杆菌(Haemophilus parasuis,HPS)血清型4、5型分离菌株经超声波破碎处理后的产物致敏醛化红细胞,建立了检测HPS抗体的间接血凝试验方法。最适反应条件为绵羊红细胞30℃醛化5 h,4、5型菌株浓缩抗原以1∶8稀释致敏红细胞。免疫猪2周后检出率达89%。对15种HPS血清型阳性血清进行检测,结果均为阳性;对猪瘟病毒、口蹄疫病毒、猪圆环病毒、猪细小病毒、猪伪狂犬病病毒、猪生殖与呼吸综合征病毒、猪肺炎支原体、猪链球菌、猪肺疫巴氏杆菌、Ⅰ相支气管败血波氏杆菌及胸膜肺炎放线杆菌阳性血清进行检测,结果均为阴性。敏感性为琼脂扩散试验的16倍。对1 665份猪血清进行检测,阳性率为47.1%。结果表明,该方法敏感性较高,特异性强,重复性好,可用于疫苗免疫后抗体水平的检测及副猪嗜血杆菌的流行病学调查。     

Validation studies of the ParaDNA® Intelligence System with artificial evidence items

Short tandem repeat (STR) profiling is one of the mostly used systems for forensic applications. In certain circumstances, STR profiling is time-consuming and costly, which potentially leads to delays in criminal investigations. LGC (Laboratory of the Government Chemist, UK) Forensics has developed a robust STR profiling platform called the ParaDNA^® Intelligence Test System which can provide early tactical intelligence and aid investigators in making informed decisions on sample prioritization for detection. Here, we validated the ParaDNA intelligence test for its application in forensic cases using a range of mock evidence items following guidelines set by the Scientific Working Group on DNA Analysis Methods (SWGDAM). Specifically, we tested the sensitivity and accuracy of the ParaDNA intelligence test, as well as the success rates for detecting mock samples and for use in case scenarios. Our findings demonstrate that the ParaDNA intelligence test generates useful DNA profiles, especially for samples such as blood, saliva, and semen that contain ample DNA, indicating the benefits of including ParaDNA as a prior step in forensic STR profiling pipelines.     

重物落水后运动过程的数学模型

为更好描述重物落水后的运动过程,设计了模拟封堵溃口小型试验,首先对重物落水后运动过程的影响因素进行分析,对采集的试验数据进行了分类处理,并对典型的试验数据进行比较分析,突出主要影响因素.其次对水中的重物进行了受力分析,以大实心方体为例,初步建立重物在水中运动过程的二阶微分方程模型（模型1）,结合步长搜索法和最小二乘法确定压差阻力系数的初始范围,然后在Matlab平台下做非线性最小二乘拟合求解运动模型中压差阻力系数kx、ky;在模型1的基础上引入速度后损率kv,完善了重物在水中运动过程的二阶微分方程模型（模型2）,并对模型中2的参数进行求解.模型结果验证显示,引入速度后损率后的模型2能比较好地反映重物落水后的运动过程,并对以后的研究提供了参考.     

视频侦查技术工作的现状和思考

视频侦查技术是继刑侦、技侦、网侦之后的第四大侦查技术,给公安工作带来了新的破案技术支撑和增长点.视频侦查技术是强化社会治安控制的有效手段,对提高侦查机关打击犯罪的效能具有重大意义.尽快将这一新兴的侦查技术广泛应用到公安实战,发挥其巨大作用,是当前研究的重点.