Maxwell 16裂解纯化法提取陈旧精子DNA的应用

目的利用Maxwell 16裂解纯化法从保存8年以上陈旧精斑检材中获取精子DNA。方法 8份陈旧精斑检材采用Maxwell 16裂解纯化法提取精子DNA,并采用Powerplex○R21试剂盒进行复合扩增,产物用AB3130型遗传分析仪检测,结果与常规方法进行对比。结果成功获得8份陈旧精斑检材精子STR分型。结论差异裂解配合Maxwell 16裂解在陈旧精斑检材精子DNA检验中效果明显。     

Default entitlements in personal data in the proposed Regulation: Informational self-determination off the table … and back on again?

This paper aims to assess the proposed General Data Protection Regulation through the framework of default entitlements in personal data. The notion of default entitlements comes from economic analysis of law and provides new insights into the implications of the data protection reform. While, under the principle of informational self-determination the default entitlements should lie with the individual, the Commission is shown to assign a great deal of default rights to others, including the Information Industry. This article cautions against the possibility of reducing the European system of data protection rooted in the values of individual autonomy and informational self-determination to a mere set of administrative rules channelling the flow of personal data, yet without a clear direction.     

The Linux FAT32 allocator and file creation order reconstruction

The allocation algorithm of the Linux FAT32 file system driver positions files on disk in such a way that their relative positions reveal information on the order in which these files have been created. This provides an opportunity to enrich information from (carved) file fragments with time information, even when such file fragments lack the file system metadata in which time-related information is usually to be found.Through source code analysis and experiments the behaviour of the Linux FAT allocator is examined. How an understanding of this allocator can be applied in practice is demonstrated with a case study involving a TomTom GPS car navigation device. In this case, time information played a crucial role. Large amounts of location records could be carved from this device's flash storage, yielding insight into the locations the device has visited—yet the carved records themselves offered no information on when the device had been at the locations. Still, bounds on the records' time of creation could be inferred when making use of filesystem timestamps related to neighbouring on-disk positions.Finally, we perform experiments which contrast the Linux behaviour with that of Windows 7. We show that the latter differs subtly, breaking the strong relation between creation order and position.     

State transformation goes nuclear: Chinese National Nuclear Companies’ expansion into Europe

Abstract

Most of the literature on state transformation focuses on China’s relations with African, Asian and Latin American countries and the National Oil Companies’ overseas expansion to show that China has become fragmented, decentralised and internationalised. This article contributes novel findings by focusing on China’s relations with Europe and the actions of China’s National Nuclear Companies (NNCs). It shows that NNCs, which have become relatively autonomous actors, often pursue their agendas of expansion into Europe without much coordination with, or even in contradiction to, other ministries’ agendas and interests, especially the Ministry of Foreign Affairs. Instead of being orchestrated by the central government, their expansion reflects considerable disorganisation and sometimes undermines China’s official strategy. The article demonstrates this through case studies of NNCs’ involvement in the UK and Romania.     

Ubuntuism as a Foundation of Media Ethics in Zimbabwe? Journalists’ Perspectives and Discontents

Abstract

There are debates on the relevance of Eurocentric normative frameworks for studying the media in post-colonial Africa. Emerging from these debates is a rebuttal of the dominant Western-derived paradigms for the conceptualisation of journalistic norms, values and practices. Given that the dominant Western liberal models for normative media ethics are incongruent to the needs of Africa, there is a growing call to reconceptualise media ethics anchored upon alternative epistemologies and moral foundations such as ubuntuism. Although there is existing scholarship on ubuntuism as a framework for media ethics in Africa, none of these studies has focused particularly on Zimbabwe. Using the 16 August 2019 (hereafter August 16) protests as a photojournalistic “moment” as a frame, this article explores the views and perspectives of Zimbabwean journalists on their understanding of media ethics and professionalism. Further, it probes the possibilities of ubuntuism as a moral foundation of journalistic practice in the country. Journalists’ views are diverse and contested on the nature and practice of media ethics in the country. Although ubuntuism is touted as a normative framework for media ethics, the Western liberal perspectives remain dominant. As such, post-colonial theory offers a useful approach to understanding the interconnections, contradictions and tensions underpinning media ethics in post-colonial Zimbabwe.     

"三八"纪念与延安时期的妇女运动

延安时期,中国共产党十分重视"三八"纪念,"三八"纪念是推动妇女运动的重要杠杆:优化纪念形式,提高妇女运动的效率;维护妇女权益,巩固妇女运动的基础;服务时代主题,提升妇女运动的价值.     

小学教师人格特征和学生学业成绩的相关研究

本运用心理学及教育生态学的方法对南京市五所小学毕业班教师人格特征与所教班级学生学业成绩作了相关研究,结果表明：小学教师的某些人格特征与学生的学业成绩有着较高相关,其中,兴奋性与学生的语成绩呈显负相关;聪慧性、稳定性、实验性与学生的教学成绩的相关以显示水平,小学教师的某些人格特征与学生的学业成绩的相关要高于小学教师的年龄、学历与学生的学业成绩的相关,小学教师的人格特征中聪慧性和兴奋性与学生的语     

深入认识和把握构建和谐社会的三个重大问题

构建社会主义和谐社会是党中央提出的社会主义现代化建设的战略目标和任务。怎样正确认识和把握和谐社会的理论和实践,科学地判断和认识当前我国出现的不和谐因素并采取积极的措施加以解决,具有重大现实意义。     

PR8流感病毒突变株的制备及其在鸡胚上生长特性的研究

在前期发现流感病毒WSN株的NS2或NP蛋白突变使病毒在细胞上增殖能力明显提高的基础上,研究了这些突变是否会使PR8病毒效价在鸡胚上进一步提高的可行性,通过反向遗传操作技术获得了NS2E67S和NPP277A两株PR8突变病毒。经过比较突变病毒与野生病毒在鸡胚中的增殖能力,发现NS2E67S突变病毒在鸡胚上增殖后的效价与HA抗原表达量均明显提高,而NPP277A突变病毒在鸡胚上的增殖能力不及野生PR8病毒。该研究结果将对进一步研究病毒基因变异与宿主互作机制奠定基础,有可能通过NS2E67S突变病毒对改良流感疫苗具有重要的应用价值。