2008～2009年浙江省猪圆环病毒2型全基因组序列的比较和分析

应用PCR方法对2008～2009年采集的99份疑似断奶仔猪多系统衰竭综合征病猪的脾、肺、淋巴结混合组织样本进行猪圆环病毒2型(PCV2)检测,并以组织DNA为模板进行了PCV2的全基因组扩增,克隆后进行了测序。结果显示,2008年与2009年浙江省PCV2的平均阳性率为47.5%,与2004～2006年相比基本持平。随机选取18份阳性病料进行PCV2全基因组测序分析,结果,18个毒株均属于Group1,其中5个毒株(27.8%)属于1A分支,3个毒株(16.7%)属于1B分支,9个毒株(50%)属于1C分支。表明,Group1依然是浙江省PCV2的主导基因型,且1C分支从无到有,并逐渐占据主导地位。     

猪圆环病毒2型对体外培养仔猪淋巴细胞转化能力及其活性的影响

采集5头PCV2和PRRSV血清抗体阴性的断奶仔猪血液,分离外周血淋巴细胞,分对照组、PHA组、LPS组、PCV2组、PCV2+PHA组、PCV2+LPS组进行体外培养,用MTT法检测PCV2作用2d后对仔猪外周血淋巴细胞转化能力的影响;无菌取脾制成单细胞悬液,分单独淋巴细胞(L)、淋巴细胞+PCV2(L+V)、淋巴细胞与巨噬细胞(L+M)和淋巴细胞与巨噬细胞+PCV2(L+M+V)4组进行体外培养.分别在培养后第0、6、12和24 h收获淋巴细胞,用RT-PCR法检测淋巴细胞IL-2R(α)mRNA的表达.结果显示,与对照组、PHA组和LPS组相比,PCV2组、PCV2+PHA组和PCV2+LPS组淋巴细胞的转化能力均极显著降低(P＜0.01);PCV2对单独培养的淋巴细胞IL-2R(α)表达的影响不显著(P＞0.05),而巨噬细胞能协同PCV2促进淋巴细胞IL-2R(α)的表达,尤其在攻毒后第12和第24 h极显著增加(P＜0.01).证实PCV2在攻毒后第2 d能抑制体外培养T、B淋巴细胞的转化能力,且巨噬细胞能协同PCV2促进T淋巴细胞的活化.     

EXCEL在交通事故分析中的应用

交通安全问题已引起了世界各国的重视,每个国家都在寻求和探索降低交通事故发生率的良策。我国是一个发展中国家,在相当长的一段时间内还会面临着严重的交通安全问题。EXCEL是一种简便、易操作的计算机软件,可以利用EXCEL的数据分析功能,求出道路交通事故中某些推测数据与多因素之间的比例关系,对道路交通事故分析有所帮助。     

Near‐fatal Intoxication with the “New” Synthetic Opioid U‐47700: The First Reported Case in the Czech Republic

Recreational use of the potent synthetic opioid 3,4‐ dichloro‐N‐(2‐(dimethylamino)cyclohexyl)‐N‐methylbenzamide (U‐47700) is rising, accompanied by increasingly frequent cases of serious intoxication. This article reports a case of near‐fatal U‐47700 intoxication. A man was found unconscious (with drug powder residues). After 40 h in hospital (including 12 h of supported ventilation), he recovered and was discharged. Liquid chromatography/high‐resolution mass spectrometry (LC/HRMS) or gas chromatography/mass spectrometry (GC/MS) were used to detect and quantify substances in powders, serum and urine. Powders contained U‐47700 and two synthetic cannabinoids. Serum and urine were positive for U‐47700 (351.0 ng/mL), citalopram (<LOQ), tetrahydrocannabinol (THC: 3.3 ng/mL), midazolam (<LOQ) and a novel benzodiazepine, clonazolam (6.8 ng/mL) and their metabolites but negative for synthetic cannabinoids. If potent synthetic opioids become cheaper and more easily obtainable than their classical counterparts (e.g., heroin), they will inevitably replace them and users may be exposed to elevated risks of addiction and overdose.     

The security of access to accounts under the PSD2

The revised Payment Services Directive (‘PSD2’) has been adopted to stimulate the development of an integrated internal market for payment services. In particular, it facilitates payment initiation services and account information services by granting the providers of these services access to the accounts of the payment service users. At the same time, the recitals state that the PSD2 guarantees a high level of consumer protection, security of payment transactions and protection against fraud.This paper answers the following question: To what extent does the access to accounts of the payment initiation service providers and account information service providers balance the development of the market for payment services with the security of the payment account and the privacy of the user? An analysis of the PSD2 shows that the development of the market for payment services has a higher priority. Security and privacy are ultimately subordinate.First, the PSD2 does not adequately protect the personal data of the users. The definition of ‘account information service’ is broad and covers a wide range of services. This allows the payment service providers to circumvent the limitations of the access to accounts.Next, the payment service providers have a ‘fall back option’ that allows ‘screen scraping’ if the dedicated interface is not functioning properly. Although this access is constrained by several safeguards, the fall back option gives the payment services provider unlimited access to the account of the user.Finally, the payment service providers have considerable freedom to arrange their authentication process as they see fit. The banks seem to be required to trust this process. The PSD2 and regulatory technical standards do not demand that a bank is able to verify the authentication or the integrity of the payment order.     

TGF-β1在SCN5A基因变异所致心源性猝死中调控作用

SCN5A基因变异所致心源性猝死发生机制尚不清楚。目前研究显示TGF-β1介导心肌纤维化以及离子通道重构调节机制的异常可能是SCN5A基因变异导致SUNDS发生的主要原因。本文就转化生长因子β1对SCN5A基因变异所致心源性猝死调控的影响机制研究进展进行综述,以期为心源性猝死法医学研究和实践提供参考。     

事件相关电位客观评估视敏度的法医学应用

目的研究不同视敏度水平的事件相关电位(event-related potential,ERP)成分特征,以期为视敏度的客观评定提供理论依据。方法选取16名被试进行单眼刺激。要求被试眼平视前方屏幕,注意屏幕中央的视觉刺激并对朝向不同的刺激进行计数。屏幕的中央会显示3种不同视敏度水平所对应的视标刺激图片,分别记录其ERP结果。结果 (1)阈上视敏度水平一致刺激的P_1振幅高于阈值、阈下视敏度水平一致刺激的P_1振幅,阈值和阈下视敏度水平一致刺激的P_1振幅差异无统计学意义,冲突刺激与一致刺激在3种视敏度水平的趋势类似。(2)阈上和阈值视敏度水平都出现了潜伏期在340~500 ms内的P300成分;阈下视敏度水平在相应的时间窗内无P300成分。3种视敏度水平的P_(300)振幅差异有统计学意义,振幅由大到小分别为阈上、阈值、阈下视敏度水平。结论 ERP技术可以成为法医学客观评定视敏度的新手段。     

"透痹转气法"对佐剂性关节炎大鼠的疗效及滑膜Fas、FasL和Bcl-2蛋白表达的影响

目的:观察"透痹转气法"对佐剂性关节炎(RA)大鼠的影响.方法:复制大鼠RA模型,设立模型组、透痹转气法组(TBZQF)、雷公藤(TPT)组、透痹逐邪胶囊(TBZXC)组;测定各组大鼠足跖肿胀抑制率、关节炎症指数;观察病变关节滑膜病理改变,并检测炎症关节滑膜组织中的Fas、FasL、Bcl-2蛋白表达.结果:与模型组比较,TBZQF组、TBZXC组、TPT组大鼠足跖肿胀抑制率、关节炎指数均显著降低(P＜0.05),而TBZQF组疗效比TBZXC、TPT组更显著(P＜0.05),且起效时间快,疗程短;各治疗组均能改善RA大鼠滑膜充血、水肿,减轻大鼠滑膜炎细胞浸润,而TBZQF组更为明显(P＜0.05);与模型组比较,各治疗组Fas、FasL的表达显著增加,Bcl-2的表达显著减少(P＜0.05),TBZQF与MTX组、TPT组比较,上述指标变化不显著(P＞0.05).结论:TBZQF治疗方案在改善佐剂性关节炎模型大鼠的关节炎症状、抑制炎症方面优于TPT和TBZXC的单一中药治疗,其作用机制可能是通过下调bcl-2基因表达调控Fas/FasL系统,促进增殖的滑膜细胞凋亡.     

城市规模灰色系统动力分析及预测

分析了柳州市城市规模变化趋势,运用灰色系统分析法对影响城市规模变化的驱动因素进行分析。研究表明:社会系统压力、经济发展、投资和工业发展是影响柳州市城市规模变化的主要驱动力。通过灰色预测模型GM(1,1),预测柳州市2010年的城市规模。