Sukzessive Zust?ndigkeit in Mietrechtsangelegenheiten

Endet ein Verwaltungsverfahren iSd § 39 MRG mit Zurückziehung des Antrages, dann fehlt für eine nachfolgende Anrufung des Gerichtes die zwingende Verfahrensvoraussetzung, dass die Sache vorher bei der Gemeinde anh?ngig gemacht wurde.     

Begünstigte Rückzahlung nach RBG 1987 – Befreiung von den Mietzinsbildungsvorschriften nach § 16 MRG

Bei einer Entscheidung über das Fondsansuchen vor dem 1. 9. 1952 kann die Befreiung von den Mietzinsbildungen des § 16 MRG nur für solche Objekte, die mit Fondsmitteln wiederhergestellt wurden, in Anspruch genommen werden.     

简论21世纪印度的发展

印度领导人提出 :2 1世纪是印度的世纪。过去 1 0— 2 0年间 ,印度的经济、军事取得了较快发展 ,国际地位得到了很大提高。但其国内社会问题严重 ,综合国力不足 ,缺乏长远外交政策 ,安全环境得不到改善 ,这些都制约了印度的进一步发展     

金丝桃素的体外抗口蹄疫病毒活性

将体外培养的BHK21细胞感染FMDV后加入金丝桃素,日光灯下光活化1 h,通过细胞病变观察、MTT法、透射电子显微镜观察、H3 UR掺入试验,探讨了光活化金丝桃素对FMDV体外生长增殖的影响。结果表明,金丝桃素有明显的抑制FMDV致BHK21 细胞病变效应,能抑制FMDV的增殖,抑制率可达61.82%。透射电镜观察,金丝桃素组与病毒对照组相比,BHK21细胞的FMDV颗粒明显减少。证实,金丝桃素在体外有明显的抑制FMDV增殖的作用。     

Phenolphthalein False‐Positive Reactions from Legume Root Nodules

Presumptive tests for blood play a critical role in the examination of physical evidence and in the determination of subsequent analysis. The catalytic power of hemoglobin allows colorimetric reactions employing phenolphthalein (Kastle‐Meyer test) to indicate “whether” blood is present. Consequently, DNA profiles extracted from phenolphthalein‐positive stains are presumed to be from blood on the evidentiary item and can lead to the identification of “whose” blood is present. Crushed nodules from a variety of legumes yielded phenolphthalein false‐positive reactions that were indistinguishable from true bloodstains both in color quality and in developmental time frame. Clothing and other materials stained by nodules also yielded phenolphthalein false‐positive reactivity for several years after nodule exposure. Nodules from leguminous plants contain a protein (leghemoglobin) which is structurally and functionally similar to hemoglobin. Testing of purified leghemoglobin confirmed this protein as a source of phenolphthalein reactivity. A scenario is presented showing how the presence of leghemoglobin from nodule staining can mislead investigators.     

中马钦州产业园区建设21世纪海上丝绸之路先行园区的战略构想

共建21世纪海上丝绸之路的发展战略对于推动和深化中国与东盟乃至亚太地区的开放合作意义重大。中马钦州产业园区在共建21世纪海上丝绸之路发展战略中有重要位置。中马钦州产业园区将充分发挥自身优势,根据中马两国领导人为其提出的"打造中马两国投资合作旗舰项目,带动两国产业集群式发展"的战略定位,将中马钦州产业园区建设成为21世纪海上丝绸之路的先行园区。     

共同建设21世纪海上丝绸之路新格局

"一带一路"是中国国家主席习近平在2013年9月、10月分别出访中亚和东南亚国家时提出的战略构想,体现了中国在全球化背景下对构建更加开放、合作,促进共同发展的区域经济新格局的战略愿景。本文认为,共建21世纪海上丝绸之路,迫切需要各方共同努力,尽快形成共同发展理念,增进政治互信,构建面向未来的发展愿景,制定发展议程,实施联合行动方案,促进互联互通。     

Developmental validation of the AmpFℓSTR® Identifiler® Plus PCR Amplification Kit: an established multiplex assay with improved performance

Abstract: Analysis of length polymorphism at short tandem repeat (STR) loci utilizing multiplex polymerase chain reaction (PCR) remains the primary method for genotyping forensic samples. The AmpF?STR^® Identifiler^® Plus PCR Amplification Kit is an improved version of the AmpF?STR^® Identifiler^® PCR Amplification Kit and amplifies the core CODIS loci: D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA. Additional loci amplified in the multiplex reaction are the sex‐determinant, amelogenin, and two internationally accepted loci, D2S1338 and D19S433. While the primer sequences and dye configurations were unchanged, the AmpF?STR^® Identifiler^® Plus PCR Amplification Kit features an enhanced buffer formulation and an optimized PCR cycling protocol that increases sensitivity, provides better tolerance to PCR inhibitors, and improves performance on mixture samples. The AmpF?STR^® Identifiler^® Plus PCR Amplification Kit has been validated according to the FBI/National Standards and Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. The validation results support the use of the AmpF?STR^® Identifiler^® Plus PCR Amplification Kit for human identity and parentage testing.     

Y-chromosome and autosomal STR diversity in four proximate settlements in Central Anatolia

Due to the longstanding human presence in the region and the influence of social traditions, the genetic make-up of populations currently inhabiting Turkey (Anatolia) is quite complex. To characterize the patterns of genetic diversity in rural Central Anatolian villages, we analyzed samples collected at four local settlements for variation at 17 Y-chromosome STR and 15 autosomal STR loci. The resulting data reveal considerable diversity within these settlements, as well as some structure in the paternal genetic variation, with a limited number of haplotypes being shared between the communities. These findings have important implications for forensic studies of Turkish populations.     

Validation of Half‐Reaction Amplification Using Promega PowerPlex® 16*

Abstract: DNA amplification is a fundamental yet costly process used in DNA analysis. This study evaluated half‐reaction amplification (12.5, 12, and 13 uL) using the Promega Powerplex^® 16 Kit with the hope of reducing sample analysis costs by half. A sensitivity study was completed, along with the testing of various blood stain samples including those with low (<0.40 ng) and high DNA concentrations (>3.0 ng), peak height imbalances, and allelic drop‐out. Also, 467 samples submitted to the MUFSC laboratory for testing were analyzed. Results indicate that half‐reaction amplification produced higher quality profiles than full‐reactions. Average peak heights increased by 85%, peak height imbalances improved, and drop‐out was eliminated in 75.8% of samples. Only eight of 467 case samples required re‐amplification, a success rate of 94% was observed, and the repeat rate decreased significantly. Finally, a DNA input of 0.25–1.0 ng is ideal for half‐reaction amplification.