脾虚大鼠胃窦、十二指肠组织5-HT细胞的变化及意义

目的P：探讨脾虚大鼠胃窦，十二指肠组织5－HT细胞的变化及意义，方法：采用免疫组织化学和医学图像分析相结合的方法，测定脾虚组，正常组，四君子汤治疗组3组大鼠胃窦，十二指肠组织5－HT细胞的数目，面积和平均灰度，结果：（1）脾虚组大鼠胃窦，十二指肠组织5－HT细胞的数目，面积数值均高于正常组，平均灰度值均较正常组降低。（2）四君子汤组的上述指标测定结果与正常组无显差异，结论：胃窦，十二指肠组织5－HT细胞的分泌功能亢进可导致消化系统功能紊乱，可能是脾虚证的重要机制之一。     

Produkthaftung für fehlerhaftes Produkt mit Schutzfunktion

Der erkennende Senat folgt der hM, wonach auch die Unwirksamkeit eines Produkts, dessen Zweck darin liegt, bestimmte Rechtsgüter vor Gefahren oder Sch?den zu schützen, als Produktfehler iSd PHG anzusehen ist. Der Schaden am fehlerhaften Produkt selbst ist nach PHG nicht zu ersetzen.     

Bezugsregeln für Kinderbetreuungsgeld verfassungskonform

Dass durch die Aufhebung einer Bestimmung als gleichheitswidrig eine Gleichheitswidrigkeit an anderer Stelle herbeigeführt würde, ist kein Prozesshindernis. Dass Kinderbetreuungsgeld auch bei mehreren gleichzeitig zu betreuenden, nacheinander geborenen Kindern nur einmal (bis zum Ablauf der Bezugsdauer für das jüngste Kind) gew?hrt wird, ist verfassungsrechtlich nicht zu beanstanden. Daran ?ndert auch die Bevorzugung von Mehrlingsgeburten nichts.     

猪泛素基因与PRRSV GP5基因融合表达质粒的构建

采用RT PCR技术从长白猪的肌肉组织中扩增出了泛素 (Ubiquitin)基因 ,并将其克隆到 pGEM Teasy载体 ,经测序表明 ,所克隆的基因与GenBank中已登录的序列同源性达 99%。利用设计的融合表达引物 ,分别将泛素基因与猪生殖与呼吸综合征病毒 (PRRSV )GP5蛋白成熟肽基因进行扩增 ,用重叠区扩增基因拼接法将 2个基因片段进行拼接 ,并插入真核表达载体 pcDNA4 .0HISMAXA中 ,构建出了 pcDNA U GP5重组质粒。     

氟乙酸五氟苄基酯的气相色谱分析

目的 对氟乙酸根阴离子的衍生物-氟乙酸五氟苄基酯(PFB-MFA)进行了气相色谱分析，以满足氟乙酰胺类鼠药化验工作的需要。方法 使用GC／ECD、GC／MS Scan、GC／MS SIM对该化合物的标准溶液进行了分析，得到了各种分析方法的校正曲线及检测限。结果 三种方法测定PFB-MFA的线性范围分别为GC／ECD 0.01～0.1ng／μl、GC／MS SCAN 1～100ng／μl、GC／MS SIM 5×10~(-3)～1ng／μl。三种方法对氟乙酸根阴离子的检测限分别为：GC／ECD1.31×10~(-4)ng／μl、GC／MS SCAN 0.13ng／μl、GC／MS SIM 1.76×10~(-4)ng／μl。结论 氟乙酸根阴离子的衍生物氟乙酸五氟苄基酯在GC／ECD及GC／MS上具有一定的线性范围，有非常高的灵敏度，可用于法庭科学的定性定量分析。     

Phenolphthalein False‐Positive Reactions from Legume Root Nodules

Presumptive tests for blood play a critical role in the examination of physical evidence and in the determination of subsequent analysis. The catalytic power of hemoglobin allows colorimetric reactions employing phenolphthalein (Kastle‐Meyer test) to indicate “whether” blood is present. Consequently, DNA profiles extracted from phenolphthalein‐positive stains are presumed to be from blood on the evidentiary item and can lead to the identification of “whose” blood is present. Crushed nodules from a variety of legumes yielded phenolphthalein false‐positive reactions that were indistinguishable from true bloodstains both in color quality and in developmental time frame. Clothing and other materials stained by nodules also yielded phenolphthalein false‐positive reactivity for several years after nodule exposure. Nodules from leguminous plants contain a protein (leghemoglobin) which is structurally and functionally similar to hemoglobin. Testing of purified leghemoglobin confirmed this protein as a source of phenolphthalein reactivity. A scenario is presented showing how the presence of leghemoglobin from nodule staining can mislead investigators.     

猪繁殖与呼吸综合征病毒GP5蛋白糖基化位点缺失突变株的构建

以含有猪繁殖与呼吸综合征病毒(PRRSV)VR-2332株全长感染性cDNA克隆的质粒pVR2332为模板,通过融合PCR方法扩增GP5蛋白第34、51位天冬酰胺糖基化位点缺失的突变序列,替换pVR2332质粒中编码GP5蛋白的DNA片段,分别获得重组质粒pVR-N34A、pVR-N51A。在体外转录后转染BHK-21细胞,转染后第48小时收集细胞接种Marc-145细胞,进行突变病毒的拯救。然后通过RT-PCR扩增拯救病毒GP5蛋白的编码序列,序列分析显示构建正确。间接免疫荧光试验证明拯救病毒能够正常表达GP5蛋白。同时,中和试验结果初步表明GP5糖基化位点缺失影响PRRSV与中和抗体的反应。猪繁殖与呼吸综合征病毒GP5蛋白不同糖基化位点缺失突变株的获得将为进一步揭示GP5蛋白糖基化与诱发机体免疫反应之间的关系奠定基础。     

Expression and function of C5a receptor in a fatal anaphylaxis after honey bee sting

The mechanisms leading to death from anaphylaxis after insect sting involve antigen cross-linkage of antibody molecules that activate immunoglobulin receptors on inflammatory cells. The aim of our study was to investigate the pathomorphology and the expression of C5aR in fatal anaphylaxis in a patient after a fatal insect sting. A 38-year-old women was stung by a honeybee. C5R1 expression was detected in many dilated capillaries in the lungs. Pulmonary epithelial cells did not bind the monoclonal antibody for C5R1; however, intensive cytoplasmic staining was detected in endothelial cells. The findings of this case provide evidence for an active role of complement in fatal anaphylaxis elicited by bee venom. C5aR detection could be useful in the identification of sudden death cases because of unwitnessed fatal insect sting cases. Authors can recommend this immunohistochemical analysis on all sudden unexpected deaths outdoors where a possible bee sting might occur.     

Developmental validation of the AmpFℓSTR® Identifiler® Plus PCR Amplification Kit: an established multiplex assay with improved performance

Abstract: Analysis of length polymorphism at short tandem repeat (STR) loci utilizing multiplex polymerase chain reaction (PCR) remains the primary method for genotyping forensic samples. The AmpF?STR^® Identifiler^® Plus PCR Amplification Kit is an improved version of the AmpF?STR^® Identifiler^® PCR Amplification Kit and amplifies the core CODIS loci: D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, CSF1PO, FGA, TH01, TPOX, and vWA. Additional loci amplified in the multiplex reaction are the sex‐determinant, amelogenin, and two internationally accepted loci, D2S1338 and D19S433. While the primer sequences and dye configurations were unchanged, the AmpF?STR^® Identifiler^® Plus PCR Amplification Kit features an enhanced buffer formulation and an optimized PCR cycling protocol that increases sensitivity, provides better tolerance to PCR inhibitors, and improves performance on mixture samples. The AmpF?STR^® Identifiler^® Plus PCR Amplification Kit has been validated according to the FBI/National Standards and Scientific Working Group on DNA Analysis Methods (SWGDAM) guidelines. The validation results support the use of the AmpF?STR^® Identifiler^® Plus PCR Amplification Kit for human identity and parentage testing.