Comparison of two methods for isolating DNA from human skeletal remains for STR analysis

Abstract: The quality and efficiency of a standard organic DNA isolation method and a silica‐based method using the QIAGEN Blood Maxi Kit were compared to obtain human DNA and short tandem repeats (STRs) profiles from 39 exhumed bone samples for paternity testing. DNA samples were quantified by real‐time PCR, and STR profiles were obtained using the AmpFlSTR^® Identifiler^® PCR amplification kit. Overall, the silica‐based method recovered less DNA ranging from 0 to 147.7 ng/g (average 7.57 ng/g, median = 1.3 ng/g) than did the organic method ranging from 0 to 605 ng/g (average 44.27 ng/g, median = 5.8 ng/g). Complete profiles (16/16 loci tested) were obtained from 37/39 samples (95%) using the organic method and from 9/39 samples (23%) with the silica‐based method. Compared with a standard organic DNA isolation method, our results indicate that the published silica‐based method does not improve neither the quality nor the quantity of DNA for STR profiling.     

The Ratio of 6β‐Hydroxycortisol to Cortisol in Urine as a Measure of Cytochrome P450 3A Activity in Postmortem Cases

Poisoning can occur with chronic accumulation of a drug due to reduced metabolic capacity; conversely, under‐treatment may occur due to an increased metabolic rate. Over half of all drugs are metabolized by the cytochrome P450 3A complex (CYP3A). The activity of CYP3A can be assessed by the urinary ratio of 6β‐hydroxycortisol to cortisol. The aim of this study was to determine the usefulness of this ratio as a postmortem marker for determining whether altered CYP3A enzyme activity occurred prior to death. In a series of 244 postmortem cases, this ratio ranged from 0.014 to 78.6 (median 3.50). The median was significantly higher (5.14) in a subgroup of 28 cases that exhibited the presence of CYP3A‐inducing drugs. In cirrhosis, the median ratio was 1.69. This pointed to a reduced metabolic capacity of CYP3A. Thus, the ratio may constitute a rough indicator of the CYP3A metabolic capacity, which could be of value in special cases.     

Maxwell 16裂解纯化法提取陈旧精子DNA的应用

目的利用Maxwell 16裂解纯化法从保存8年以上陈旧精斑检材中获取精子DNA。方法 8份陈旧精斑检材采用Maxwell 16裂解纯化法提取精子DNA,并采用Powerplex○R21试剂盒进行复合扩增,产物用AB3130型遗传分析仪检测,结果与常规方法进行对比。结果成功获得8份陈旧精斑检材精子STR分型。结论差异裂解配合Maxwell 16裂解在陈旧精斑检材精子DNA检验中效果明显。     

The Linux FAT32 allocator and file creation order reconstruction

The allocation algorithm of the Linux FAT32 file system driver positions files on disk in such a way that their relative positions reveal information on the order in which these files have been created. This provides an opportunity to enrich information from (carved) file fragments with time information, even when such file fragments lack the file system metadata in which time-related information is usually to be found.Through source code analysis and experiments the behaviour of the Linux FAT allocator is examined. How an understanding of this allocator can be applied in practice is demonstrated with a case study involving a TomTom GPS car navigation device. In this case, time information played a crucial role. Large amounts of location records could be carved from this device's flash storage, yielding insight into the locations the device has visited—yet the carved records themselves offered no information on when the device had been at the locations. Still, bounds on the records' time of creation could be inferred when making use of filesystem timestamps related to neighbouring on-disk positions.Finally, we perform experiments which contrast the Linux behaviour with that of Windows 7. We show that the latter differs subtly, breaking the strong relation between creation order and position.     

3D打印治安管理对策研究

3D打印技术如今已受到了社会各界广泛的关注,有着良好的发展前景。然而其本身也具有着一定的治安隐患,如危险物品泛滥、特殊物品复制等。对于3D打印技术,需要进行系统的风险评估,并通过完善机制的构建进行人为控制,以保证其健康发展。这一机制的构建,除了应运用较多的高科技手段外,也应当在管理规范层面做到有序、协调。     

The High Representative and directoires in European foreign policy: the case of the nuclear negotiations with Iran

ABSTRACT

The negotiations with Iran about its nuclear programme have seen the most protracted involvement of the High Representatives of the European Union in a high-profile policy case. This article traces the evolution of the High Representatives’ participation in the negotiations, from the first contacts with the E3 (Germany, France and the United Kingdom) in 2003 to the adoption of the Joint Comprehensive Plan of Action in 2015. It focuses on the institutional role the High Representatives played in relation with the directoires leading the talks with Iran – first the E3 and, since 2006, E3/EU. In this context, it examines the personal and organisational factors that affected the influence each of the three High Representatives (Javier Solana, Catherine Ashton and Federica Mogherini) had in regard to the directoires. The analysis distinguishes specifically between the phases before and after the 2009 Lisbon Treaty. The article shows how similar personal qualities of the three High Representatives in terms of problem-solving and trust-building gave them political capital that enabled them to adopt a fairly constant role as bridge-builders within the directoires and between the directoires and other actors. The reforms of the Lisbon Treaty had only a minor impact.     

State transformation goes nuclear: Chinese National Nuclear Companies’ expansion into Europe

Abstract

Most of the literature on state transformation focuses on China’s relations with African, Asian and Latin American countries and the National Oil Companies’ overseas expansion to show that China has become fragmented, decentralised and internationalised. This article contributes novel findings by focusing on China’s relations with Europe and the actions of China’s National Nuclear Companies (NNCs). It shows that NNCs, which have become relatively autonomous actors, often pursue their agendas of expansion into Europe without much coordination with, or even in contradiction to, other ministries’ agendas and interests, especially the Ministry of Foreign Affairs. Instead of being orchestrated by the central government, their expansion reflects considerable disorganisation and sometimes undermines China’s official strategy. The article demonstrates this through case studies of NNCs’ involvement in the UK and Romania.     

Ubuntuism as a Foundation of Media Ethics in Zimbabwe? Journalists’ Perspectives and Discontents

Abstract

There are debates on the relevance of Eurocentric normative frameworks for studying the media in post-colonial Africa. Emerging from these debates is a rebuttal of the dominant Western-derived paradigms for the conceptualisation of journalistic norms, values and practices. Given that the dominant Western liberal models for normative media ethics are incongruent to the needs of Africa, there is a growing call to reconceptualise media ethics anchored upon alternative epistemologies and moral foundations such as ubuntuism. Although there is existing scholarship on ubuntuism as a framework for media ethics in Africa, none of these studies has focused particularly on Zimbabwe. Using the 16 August 2019 (hereafter August 16) protests as a photojournalistic “moment” as a frame, this article explores the views and perspectives of Zimbabwean journalists on their understanding of media ethics and professionalism. Further, it probes the possibilities of ubuntuism as a moral foundation of journalistic practice in the country. Journalists’ views are diverse and contested on the nature and practice of media ethics in the country. Although ubuntuism is touted as a normative framework for media ethics, the Western liberal perspectives remain dominant. As such, post-colonial theory offers a useful approach to understanding the interconnections, contradictions and tensions underpinning media ethics in post-colonial Zimbabwe.     

The use of 3D visualization for sustainable tourism planning

This paper describes the results of using 3D visualization (3DV) in sustainable tourism planning (STP) during the Comcot project in Estonia. Organizers were interviewed on the characteristics of 3DV for STP and on how participants were involved in discourse using 3DV. The study describes experiences from the Setu, Maidla, and Võrtsjärv lake regions of Estonia. The content analysis of the interviews suggested that the precise selection of visualization objects, detalization levels, and user interface characteristics may significantly influence the quality of STP sessions and help to optimize time, financial, and human resources allocated for a project. The coherent design of the 3DV tool and the neutral moderator positively affect the level of participant involvement in STP and support a trusted platform for community decision making.     

颅骨三维模型制作和数据库的构建

目的采用激光扫描深度图像法和CT影像法,制作颅骨模型,并建立无身源颅骨模型数据库。方法收集各省(区)送检的59例无身源颅骨样本,采用三维激光扫描仪进行颅骨深度图像的采集;采用多排螺旋CT进行颅骨断层影像数据的采集。分别经处理后建立颅骨三维模型。利用3D打印技术实现数字颅骨模型的实体复制。建立具有颅骨信息查询和三维模型显示的无身源颅骨模型数据库。结果采用深度图像法可以获得颅骨的外表面模型,且通过调整扫描仪的参数使细节特征更清晰。采用CT断层影像法可以重建颅骨的内、外部结构,且对于牙齿具有更好的重建结果。建立数据库并将59例无身源颅骨样本信息和三维模型纳入数据库。结论三维建模和3D打印技术可实现对颅骨的重建和模型复制。无身源颅骨数据库的构建,可实现存储和管理颅骨样本的各类信息和三维数据,有助于查询和信息共享,对法医学实践有积极的应用价值。