Bcl-2蛋白在外伤性癫痫病灶中的表达

目的观察Bcl-2蛋白在外伤性癫痫病灶中的表达变化,探讨其作用机制。方法收集外伤性癫痫、非外性癫痫患者及交通事故死亡者脑皮质各15例,采用免疫组织化学和荧光免疫组织化学技术,观察各实验组Bcl-2蛋白表达水平,测定阳性表达的灰度值,数据采用SPSS 12.0软件进行统计分析。结果外伤性及非外伤性癫痫组Bcl-2蛋白表达水平均高于交通事故死亡组（P〈0.05）;而非外伤性癫痫组表达明显高于外伤性癫痫组,差异亦具有显著统计学意义（P〈0.05）。结论 Bcl-2蛋白能抑制神经元凋亡,对外伤性癫痫的发病过程具有抑制作用。     

TW2法3种标准用于中枢性性早熟患儿骨龄推断的比较

目的 探讨TW系列骨龄评分法中TW2(英国人标准)、TW2-CHN(中国人标准)和TW2-JP(日本人标准)3种标准对中枢性性早熟(central precocious puberty,CPP)惠儿骨龄评价的诊断价值,以及正常值临界点的确定.方法 选取确诊为CPP的病例组61例;对照组67例,其中同龄正常儿童61例,另6例为特殊对照组.两名医师独自阅每一对象左手腕部X线片,分别用3种标准测定骨龄;将骨龄与生理年龄的差值(d)用SPSS 13.0进行ROC分析.结果 (1)两名医师骨龄测定结果的K值为0.776(u=16.128,P<0.05):(2)TW2、TW2-CHN及TW2-JP 3种标准测得的ROC曲线下面积两两之间的差异无统计学意义;(3)TW2中d≥1.15岁、TW2-CHN中d≥1.25岁与TW2-JP中d≥0.65岁为敏感度、特异度都较高的点.结论 TW2、TW2-CHN及TW2-JP 3种标准时CPP患儿骨龄评价的诊断价值都较高,其中TW2-CHN对中国儿童的诊断价值最大.     

3个miniSTR基因座荧光标记复合扩增体系的建立及应用

目的建立扩增片段小于115 bp,包括D1S1676、D6S1274和D17S1299 3个非CODIS系统的miniSTR基因座复合扩增系统,用于高度降解DNA样本的基因分型。方法采用不同荧光染料标记引物,通过PCR扩增,利用310遗传分析仪对100份成都汉族健康无关个体血样以及2份高度降解检材进行检测。结果荧光标记复合扩增D1S1676、D6S1274和D17S1299 3个miniSTR基因座,每个基因座均获得了清晰的基因型分型结果。100份样本,3个miniSTR基因座分别检出个9、9、7个等位基因和27、23、18种基因型,基因型分布均符合Hardy-Weinberg平衡。3个基因座在成都汉族人群的累积非父排除率、累积个体识别能力分别为0.9991和0.9160。结论本系统可以应用于个体识别和亲权鉴定,为DNA高度降解样本分型提供了新的方法。     

新疆维吾尔族群体D6S1043、D12S391基因座遗传多态性

本研究调查了居住在新疆境内的677个维吾尔族无关个体D6S1043、D12S391基因座的遗传多态性,现报道如下。 1材料与方法 677份无关个体血样全部来自于日常检案,采耳垂静脉血制成血痕样本保存。血样采取Chelex-100法提取。     

华东汉族人群乙醇代谢酶相关SNP位点的多态性

目的对ADH2、ADH3、ALDH2和CYP2E1基因的40个SNP位点进行群体遗传学分析,得到多态性信息。方法利用PCR和质谱技术平台对SNP位点进行分型检测,通过对中国华东地区汉族人群199个无关个体的调查,统计分析40个SNP位点的等位基因分布频率。结果 40个SNP位点中,rs698、rs2241894（ADH3基因座）,rs13306164、rs671（ALDH2基因座）和rs28371746、rs2515641（CYP2E1基因座）的小等位基因分布频率（MAF）均大于1%,其它SNP位点的MAF均小于1%。结论 ADH2、ADH3、ALDH2和CYP2E1基因的40个SNP位点中,6个位点（rs698、rs2241894、rs13306164、rs671、rs28371746和rs2515641）在华东汉族人群中具有多态性。     

C2C在线交易合同的行为制度和法律适用研究

本文主要关注于具体的C2C在线交易行为,特别强调了在线交易人充分信息披露的重要性,并对交易双方当事人如何进行合理的信息披露展开了论述。对C2C这种交易合同订立过程中所呈现出的交易特点,从现有的法规范出发,寻找与这种交易过程的契合点,达到了比较好的互动。     

Political Ideology,Political Party,and Support for Greater Federal Spending on Environmental Protection in the United States: Evidence from the General Social Surveys, 1993–2018

This paper focuses on the effects of political ideology and party affiliation on support for more government spending on environmental protection. Pooled‐sample results show that Liberals (Democrats) are more likely to support higher government spending on environmental protection than Moderates (Independents), who, in turn, are more likely to support higher spending levels than Conservatives (Republicans). The results persist even when we control for respondents' opinions concerning whether the federal government, in general, does too little or too much. When stratifying by party, ideological divisions generally narrow, while stratifying by ideology leads to slightly wider divisions between Democrats and Republicans. Together, these results suggest that when Liberals and Conservatives form opinions about government spending on the environment, party affiliation, to some degree, dampens the effects of ideology. Between 2014 and 2018 the probability of supporting more environmental spending increased, albeit slightly, for all ideologies and parties, but more so for Liberals and Democrats.     

通督调神针灸疗法治疗遗忘型轻度认知功能障碍临床观察

目的 观察通督调神针灸疗法对遗忘型轻度认知功能障碍（amnestic mild cognitive impairment,aMCI）患者认知功能的改善作用,探究其部分作用机制。方法 将60例aMCI患者随机分为治疗组和对照组,每组30例。治疗组患者接受通督调神针灸疗法,对照组患者口服尼莫地平,两组疗程均为8周。治疗前后分别采用简易精神状态量表（mini-mental state examination,MMSE）、蒙特利尔认知评估（Montreal cognitive assessment,MoCA）量表和血管性痴呆辨证量表（the syndrome differentiation scale of vascular dementia,SDSVD）评估认知功能,并检测血清超敏C-反应蛋白（high sensitivity C-reactive protein,hs-CRP）和白细胞介素-6（interleukin-6,IL-6）水平。结果 两组治疗后MMSE、MoCA评分均较治疗前显著升高（P<0.05）,血清hs-CRP和IL-6水平均较治疗前显著降低（P<0.05）,且治疗组治疗后SDSVD评分较治疗前显著降低（P<0.05）。治疗组治疗后MMSE升高值和SDSVD、IL-6降低值均显著大于对照组（P<0.05）。结论 通督调神针灸疗法可明显改善aMCI患者的认知功能,其部分机制与降低血清IL-6有关。     

刺血拔罐联合蛇伤冲剂治疗蝮蛇咬伤临床观察

目的 观察刺血拔罐联合蛇伤冲剂治疗蛇咬伤患者的临床疗效。方法 采用随机数字表法将60例蛇咬伤患者分为对照组和观察组各30例,对照组给予西医基础治疗,观察组在对照组基础上加用刺血拔罐和内服蛇伤冲剂,评估患肢肿胀、疼痛消失时间并检测治疗前后血清超敏C反应蛋白（high-sensitive C-reactive protein,hs-CRP）、白细胞介素-6（interleukin-6,IL-6）水平。结果 观察组患者治疗后肿胀、疼痛症状的改善程度优于对照组（P<0.05）;与治疗前比较,两组治疗后hs-CRP、IL-6均显著降低（P<0.05）。两组治疗后hs-CRP降低值比较,差异无统计学意义（P>0.05）,观察组IL-6降低值显著大于对照组（P<0.05）。结论 刺血拔罐联合蛇伤冲剂内服可以改善蛇咬伤后肿胀、疼痛的症状,降低血清hs-CRP、IL-6水平。     

FETAL TESTOSTERONE AND CRIMINALITY: TEST OF EVOLUTIONARY NEUROANDROGENIC THEORY

Evolutionary neuroandrogenic (ENA) theory asserts that criminality is a crude form of competitive behavior over resources, status, and mating opportunities. Theoretically, males have been selected for resource acquisitiveness as a result of female preferences for mates who are successful at resource provisioning. ENA theory also asserts that brain exposure to both prenatal and postpubertal androgens (particularly testosterone) promotes all forms of competitiveness, including those that victimize others. The present study was undertaken to test ENA theory by correlating 14 self‐reported measures of offending with a biomarker for fetal testosterone exposure based on the ratio of the 2nd and 4th digits of the right hand (r2D:4D), in a nonrepresentative sample of 445. Both Spearman correlations and negative binomial regressions produced results that largely supported the hypothesized connection between offending and high prenatal androgen exposure, even when findings were analyzed separately by sex. Also, offending was significantly associated with r2D:4D for both males and females. Overall, this study supports the view that exposing the brain to high levels of testosterone and other androgens prenatally elevates the probability of offending later in life.