冠状动脉内弹力膜缺损与冠心病猝死的关系

目的探讨冠状动脉内弹力膜(IEL)病变与冠心病猝死(SCD)的关系。方法从本单位近5年尸检案例中挑选60例病例和心脏标本,分为SCD组、对照组A和B组。每例分别在冠状动脉左前降支近段取材,常规制片,HE和双重组合特殊染色,显微镜下观察冠状动脉IEL,并用图像分析技术对特殊染色的IEL进行形态定量分析。结果60例中,SCD组(男性24例,女性6例,年龄30~83岁)的冠状动脉IEL缺失明显(缺失率=41.64%),未缺失的IEL失去原有弯曲度而变平直;对照组A(冠心病非猝死者,男性13例、女性2例,年龄41~80岁)的冠状动脉IEL缺失程度较SCD组稍轻(缺失率=30.53%);对照组B(无冠心病的其他死者,男性8例,女性7例,年龄17~54岁)的冠状动脉IEL仅有轻度缺失(缺失率=15.67%),多保持原有波浪状结构。统计学分析显示,SCD组与对照组、SCD组与对照组B、对照组A与对照组B之间IEL缺损率的差异均具有极显著性意义;SCD组与对照组A在IEL缺损率上的差异有显著性意义。结论SCD与其冠状动脉IEL缺损率的大小密切相关。     

堆型艾美球虫广东株3-1E基因的克隆与序列分析

根据GenBank中登录的堆型艾美球虫31E基因序列,设计了3条引物,以广东株堆型艾美球虫裂殖子总RNA为模板,利用反转录聚合酶链反应(RTPCR)扩增获得了31E基因部分片段,将这一片段克隆至pGEMTEasy载体中,经PCR、限制性内切酶分析和克隆片段的序列测定、比较,证实了克隆片段的可靠性。序列比较发现,所克隆的基因片段与Eimeriaacervulina美国株(US)、E.acervulinaQH株31EcDNA的核苷酸同源性分别为99.0%和99.2%,推导氨基酸的同源性分别为98.2%和97.6%。     

鸡肾型传染性支气管炎病毒的分离鉴定

从陕西省榆林市某蛋用种鸡场疑似肾型传染性支气管炎病鸡中分离到了1 株肾型IBV(定名为YL 04),并对分离病毒进行了血凝性、血凝抑制性、致病性、鸡胚矮小化、电镜特征等生物学特性鉴定及S1基因5′端的RT PCR鉴定。结果表明,该分离株经10 g/L胰酶处理后的各代病毒尿囊收集液均可凝集鸡红细胞;标准阳性血清可特异性地抑制其凝集性;可复制出与自然发病相同的病例;病毒传代物有明显的致鸡胚矮小化作用;透射电镜下可见有近似球形、直径100 nm左右的冠状病毒粒子;用RT PCR方法扩增到1 条373 bp的目的片段,其核苷酸序列与IBV CQ/01/2004株序列的同源性达98%。     

High performance liquid chromatographic determination of alcohols with reference to body distribution of methanol

A method has been developed on reverse phase high performance liquid chromatography for simultaneous determination of methyl, ethyl and isopropyl alcohols under refractive index detection using pure water as the mobile phase. A good separation has been achieved between these alcohols. Detector response was linear with a detection limit of 5 mg/100 ml. Recovery studies were performed by adding known amounts of methyl and ethyl alcohols to blood, lung and liver within the range 80–90%. The reproducibility of the results was always >90%. The quantitative distribution of methyl alcohol in postmortem body tissues and fluids has been reported in three cases of poisoning.     

Indigenismo and the Avant‐garde: Jorge Sanjinés' Early Films and the National Project

Jorge Sanjinés' 1960s films Revolución and Ukamau challenge the class and ethnic hierarchies of Bolivian society by casting the proletarian and indigenous masses as revolutionary liberators. The new national imaginary they evoke is tightly bound to the experimental cinematic techniques they employ, since their rejection of rationalist, realist aesthetics signals a partial undermining of the linear time of the modern nation. Ukamau both recalls and resists previous Bolivian indigenismo, which sought to co‐opt the Indian into a national mestizo consciousness. Its exoticist portrayal of the Indian ultimately limits its political effectiveness, but textual and contextual analyses show subversive Indian agency leaking through.     

A CROSS‐NATIONAL TEST OF BONGER'S THEORY OF CRIMINALITY AND ECONOMIC CONDITIONS*

Using international data for 100 countries, we test two hypotheses derived from Bonger's Marxian theory of crime. The analyses support the hypothesis that the degree of capitalism significantly predicts homicide rates, but they fail to confirm that the de‐moralization of the population (loss of moral feelings for others) mediates the relationship between capitalism and homicide. Although capitalism is not the best predictor among those considered, overall, the results underline the importance of Bonger's ideas because both capitalism and corruption (our indicator of de‐moralization) show reasonably strong relationships with homicide rates and compete with other variables commonly used as predictors of international homicide rates. The results confirm the usefulness of attempting to subject Marxian ideas to positivist, quantitative tests, with an eye to integrating Marxian theories with other mainstream theories, such as institutional anomie theory.     

口蹄疫病毒3D基因的真核表达及其表达产物的功能分析

利用RT-PCR技术扩增口蹄疫病毒(FMDV)编码RNA依赖的RNA聚合酶的3D基因,经测序确认后将其克隆到真核表达质粒载体pcDNA3.1( )中,重组质粒pcDNA3.1( )-3D经HindⅢ、XbaⅠ双酶切后转染至BHK-21细胞,用纯化的目的蛋白进行Western-blotting鉴定,并预测该3D基因表达产物的三级结构。结果显示,获得分子质量约55 ku的单一3D基因表达产物,其三级结构较为保守。利用RNA细胞内复制体系和荧光定量RT-PCR技术,证明3D基因表达产物在细胞内可促进口蹄疫病毒基因组的复制。     

Klagsanmerkung zwecks Realisierung des gesetzlichen Vorzugspfandrechts gem § 27 Abs 2 WEG 2002 nach Veräußerung des WE-Objekts durch den Bekl?

Die Klagsanmerkung zwecks Ausnützung des gesetzlichen Vorzugspfandrechts (hier: der Eigentümergemeinschaft) setzt nach § 27 Abs 2 WEG 2002 voraus, dass sich die Klage und der Antrag auf Klagsanmerkung gegen einen Bekl richten, der zu diesem Zeitpunkt noch als Wohnungseigentümer im Grundbuch eingetragen ist. Die bewilligte Grundbuchseintragung wirkt auf den Zeitpunkt des beim Grundbuch einlangenden bücherlichen Antrags/Grundbuchsgesuchs zurück. Deshalb schlie?t auch das zeitlich vor dem Klagsanmerkungsantrag eingebrachte, aber erst nach dessen Einlangen beim Grundbuchsgericht bewilligte Einverleibungsgesuch eines Dritten, WE im Grundbuch zu erwerben, die Klagsanmerkung gegen den Ver?u?erer aus. über den sp?ter eingebrachten Anmerkungsantrag darf vielmehr erst entschieden werden, wenn das Einverleibungsgesuch des WE-Erwerbers rechtskr?ftig bewilligt ist.