Description of the peasants’ sexual behavior in August Wilhelm Hupel’s Topographical Messages in the context of the history of the Estonian family

The aim of this article is to introduce the work of August Wilhem Hupel, a Baltic German author of the 18th century. The article focuses on the sections in his most voluminous work Topographische Nachrichten von Lief- und Ehstland (1774–1782) that describe Estonian peasants’ sexuality. Hupel’s writings belong to the sources of the history of the Estonian family, a field that has been rediscovered at the beginning of the 21st century. Thanks to the work of Marika Mägi and Nils Blomkvist, a new paradigm for research in this field has been opened up. This article adheres to that paradigm.     

鹅IL-18基因的克隆和原核表达及其多克隆抗体的制备

参考GenBank中鸡、火鸡和鸭IL-18基因序列,设计了1对特异引物,以提取的鹅脾细胞总RNA为模板,应用RT-PCR扩增、克隆鹅IL-18基因中间的333bp序列。序列分析结果表明,其与鸭IL-18基因对应序列相似性最高,达99%。参考鸭IL-18全基因序列设计上、下游引物各3条,以梯度PCR探索扩增、克隆鹅IL-18全基因。以上述策略成功扩增、克隆了3个广东地方杂交品种鹅的IL-18基因。序列分析表明,这3个杂交品种鹅的IL-18基因序列一致,长642bp,含一个603bp的ORF,编码200个氨基酸,分子质量为23.2ku。ORFN端的30个氨基酸为前导序列,其余170个氨基酸为功能蛋白序列。在GenBank中注册了第一条鹅的IL-18基因序列,登录号为EF159728。随后,构建了鹅IL-18基因ORF的重组原核表达质粒pET-GIL-18,经诱导表达、纯化获得重组鹅IL-18融合蛋白,并以其制备了兔抗重组鹅IL-18多克隆抗体。     

The Future of Consumer Web Data: A European/US Perspective

The article considers the subject of clickstream data from aEuropean/US perspective, taking into account the Data ProtectionFramework (Data Protection Directive 95/46/EC; Directive onPrivacy and Electronic Communications 2002/58/EC) and the USlegal framework and in particular, the Wiretap Act U.S.C. 2701(2004) and related statutes. It examines the extent to whichclickstream data is considered "personal data" within the DataProtection Directive and the implications to consumers and businesses.     

Fall der "wirkungsgebundenen" einheitlichen Streitpartei im Außerstreitverfahren über einen abweichenden Aufteilungsschlüssel nach § 32 Abs 5 WEG 2002

Setzt der Au?erstreitrichter rechtsgestaltend den Aufteilungsschlüssel für die Kosten eines Personenaufzugs auf Antrag eines Wohnungseigentümers abweichend vom Anteilsverh?ltnis fest, bindet sein Sachbeschluss gem § 32 Abs 5 WEG 2002 s?mtliche zum Zeitpunkt der Rechtskraft verbücherten Wohnungseigentümer. Dies bewirkt einen Anwendungsfall der sog "wirkungsgebundenen" einheitlichen Streitpartei aller Gemeinschafter. Da es auf die tats?chliche Nutzung von Gemeinschaftsanlagen durch einen Wohnungseigentümer nicht ankommt, bildet ausschlie?lich deren objektive und nicht die subjektive Nutzungsm?glichkeit den Ma?stab für den vom Au?erstreitrichter festzusetzenden, von § 32 Abs 1 WEG 2002 abweichenden Aufteilungsschlüssel. Dessen Ermessensentscheidung setzt erhebliche Unterschiede in der objektiven Nutzungsm?glichkeit durch einen Wohnungseigentümer voraus.     

18世纪中越边境贸易中的入越华商

中越边境贸易在18世纪达到一个高峰,早期以越人来商为主的情况发生变化,华商入越成为主流。入越华商主要由广西、云南边境地区的本土边民和其他外省商人构成,他们在中越贸易的过程中,逐渐当地化。华商的贸易活动对中越两国的经济社会发展均产生了深刻影响。     

Russia,the death penalty,and Europe: the ambiguities of influence

Studies of capital punishment worldwide investigate how international influence affects the death penalty. We analyze European influence on the death penalty in Russia over the imperial, Soviet, and post-Soviet periods, using two parameters: the changing mechanisms of influence in each period and the death penalty's significance in the broader spectrum of punitive violence. On the first parameter, in the tsarist period, European influence on Russian policy was “productive” – exercised through prestige, moral suasion, and “diffusion.” In the Soviet period, European influence was blocked. In the post-Soviet period, European influence is coercive, as the Council of Europe has unsuccessfully sought to compel Russia to abolish its death penalty. On the second parameter, the death penalty in Russia has always been only one of many forms of state-sanctioned punitive killing. In consequence, the Council's involvement in Russia's death penalty has produced an incoherent policy outcome and has entangled the Council in Russia's authoritarian politics. Russia thus exemplifies the hazards of external involvement in death penalty abolition.     

指印印面特征的运用——CNAS-T18指印项目结果解析

通过对CNAS-T18指印项目实施与结果的解析,讨论了当前国内利用指印印面特征的现状：民事案件指印鉴定基础理论的不足。提出了对民事案件指印特征重新进行分类研究,以及探讨、规范利用指印印面特征步骤、方法的必要性和紧迫性。强调了指印印面特征在解决民事案件中的重要作用。     

Soil sample metagenome NGS data management for forensic investigation

Soil analysis is a valuable resource in forensic investigation. Classical forensic soil analysis involves examination of its physical characteristics and chemical composition, such as soil type, colour, particle size, shape, pH, elemental, mineral and organic content. However the limited variability of these parameters is not always allowing adequate discrimination between soil samples. As soil supports extreme diversity of microorganisms and eukaryotic communities, microbiological approaches have been proposed. Several molecular approaches for microbial DNA profiling are available; however there is a lack of published data of implementation of the next generation sequencing (NGS) approaches for forensic soil analysis.The aim of the current study was elaboration of criteria for soil metagenome data management and database searching. We used our previously sequenced collection of 11 samples collected from different environments (forests, fields, grasslands, urban park) with different flora. The single sample collection includes 9 soil samples per one sampling area (30 m × 30 m) spaced by 15 m. In the current study we concentrated mainly on 18S rRNA gene V2-V3 region for fungi however SSU rRNA region for arbuscular mycorrhizal (AMF) fungi and V2-V3 hypervariable region of 16S rRNA gene for bacterial communities were taken into account. The sequencing was performed by Roche/454 platform. For data analysis OTU based approach on mothur software and NCBI BLASTN search were used. NCBI BLASTN analysis revealed altogether 2983 AMF matches and 8997 18S matches as well as 25477 OTUs (16S) were determined. Several data filtration approaches were used for data management. We found that 18S marker results could be used to create and run a filtered database that is computationally much more efficient and flexible. Our results have broad impact; however more samples have to be analysed, additional studies performed and cooperation between soil scientists and forensic scientists is required to be able to implement these novel techniques into the routine forensic practice.     

坚定中国特色社会主义自觉自信

坚定中国特色社会主义自觉自信,源于中国特色社会主义发展成型、源于辉煌成就、源于对现实的深刻把握和自我反省、源于探索实践创新,是全体中国人民的共同心声,是中国共产党代表全体中国人民在党的十八大上向世人发出的最强音!     

坚定不移高举中国特色社会主义伟大旗帜

中国特色社会主义伟大旗帜具有完整而科学的内涵。旗帜是政治方向,道路是实现途径,理论体系是行动指南,制度是根本保障。