Description of the peasants’ sexual behavior in August Wilhelm Hupel’s Topographical Messages in the context of the history of the Estonian family

The aim of this article is to introduce the work of August Wilhem Hupel, a Baltic German author of the 18th century. The article focuses on the sections in his most voluminous work Topographische Nachrichten von Lief- und Ehstland (1774–1782) that describe Estonian peasants’ sexuality. Hupel’s writings belong to the sources of the history of the Estonian family, a field that has been rediscovered at the beginning of the 21st century. Thanks to the work of Marika Mägi and Nils Blomkvist, a new paradigm for research in this field has been opened up. This article adheres to that paradigm.     

Development and validation of the AmpFlSTR MiniFiler PCR Amplification Kit: a MiniSTR multiplex for the analysis of degraded and/or PCR inhibited DNA

DNA typing of degraded DNA samples can be a challenging task when using the current commercially available multiplex short tandem repeat (STR) analysis kits. However, the ability to type degraded DNA specimens improves by redesigning current STR marker amplicons such that smaller sized polymerase chain reaction (PCR) products are generated. In an effort to increase the amount of information derived from these types of DNA samples, the AmpFlSTR MiniFiler PCR Amplification Kit has been developed. The kit contains reagents for the amplification of eight miniSTRs which are the largest sized loci in the AmpFlSTR Identifiler PCR Amplification Kit (D7S820, D13S317, D16S539, D21S11, D2S1338, D18S51, CSF1PO, and FGA). Five of these STR loci (D16S539, D21S11, D2S1338, D18S51, and FGA) also are some of the largest loci in the AmpFlSTR SGM Plus kit. This informative nine-locus multiplex, which includes the gender-identification locus Amelogenin, has been validated according to the FBI/National Standards and SWGDAM guidelines. Our results demonstrate significant performance improvements in models of DNA degradation, PCR inhibition, and nonprobative samples when compared to the AmpFlSTR Identifiler and SGM Plus kits. These data support that the MiniFiler kit will increase the likelihood of obtaining additional STR information from forensic samples in situations in which standard STR chemistries fail to produce complete profiles.     

党的十八大以来意识形态建设的伟大创新和基本经验研究

党的十八大以来,以习近平同志为核心的党中央把建设具有强大凝聚力和引领力的社会主义意识形态摆在全局工作的重要位置,作出一系列重大决策,实施一系列重大举措,既进行了彪炳史册的创新创造,取得了极其伟大的成就,也积累了丰富的宝贵的历史经验,在实践中不断深化对意识形态建设的规律性认识,为开启全面建设社会主义现代化强国新征程提供了坚强思想保证和强大精神力量。