应用反转录-聚合酶链反应检测动物组织中的口蹄疫病毒

应用反转录－聚合酶链反应（ＲＴ－ＰＣＲ）技术检测了猪和牛的扁桃体、淋巴结、脊髓、肌肉和蹄冠皮肤中的口蹄疫病毒（ＦＭＤＶ）。与接种乳鼠测毒法比较，该方法的灵敏度达０．１６ＬＤ５０～０．３２ＬＤ５０病毒量。检测人工感染后７～３５ｄ的猪组织样品７７份，２５份为阳性；接种乳鼠并盲传３代，乳鼠－间接血凝试验（ＩＨＡ）鉴定６份为阳性。检测人工感染后４～７ｄ的牛组织样品５２份，２０份为阳性；接种乳鼠－ＩＨＡ鉴定１２份为阳性。检测野外鲜肉样品４７份，１２份为阳性；任选其中８份样品接种细胞单层，盲传至第６～７代，先后有５份样品产生ＣＰＥ；其中６份样品同时接种乳鼠，盲传至第６～７代，３组乳鼠出现ＦＭＤＶ致病症状，ＩＨＡ鉴定为阳性。检测冻猪肉样品３７份，３份为阳性；冻牛肉样品９份、冻羊肉样品１０份，均为阴性     

试论毒品来源同一认定的理论基础

毒品来源同一认定是我国当前毒品检验工作中一个亟待解决的重大课题。从物证理化检验中种属认定的哲学基础上,可表明特征反映体的发掘和表达与种属认定范围之间的正相关关系,以及毒品海洛因的杂质和稀释物在新一鉴定层次中的作用。只要正确认识事物发展、变化的客观规律,充分发挥现代科学技术的作用,就一定能不断拓展我们的视野,把毒品检验的种属认定引向深入,以达到不同层次的“同一”。     

美国与日本的反毒政策可借镜之观点与对策（节选）

近年来 ,台湾面临严重的药物滥用问题 ,对社会治安、民众健康与青少年生存发展 ,均造成重大之伤害 ,实为当前政府与社会之重要课题。美日两国亦颇为毒品所苦 ,其两国对毒品之防治亦不遗余力。他山之石或可攻玉。本文旨在探讨美日两国药物滥用之防治措施 ,并借由归纳两国之防治措施以提供评估相似策略之参考     

与时俱进——理论与现实之间的张力

与时俱进,首要的是思想观念和实践上要“进”,用发展着的马克思主义指导新的实践;根据当代社会生产的特点深化对劳动和劳动价值论的认识,最广泛最充分地调动一切积极因素;强化执政党的政绩意识;以改革的精神推进党的建设。     

GC–MS studies on acylated derivatives of 3-methoxy-4-methyl- and 4-methoxy-3-methyl-phenethylamines: Regioisomers related to 3,4-MDMA

A series of side chain regioisomers of 3-methoxy-4-methyl- and 4-methoxy-4-methyl-phenethylamines have mass spectra essentially equivalent to the controlled drug substance 3,4-methylenedioxymethamphetamine (3,4-MDMA), all have molecular weight of 193 and major fragment ions in their electron ionization mass spectra at m/z 58 and 135/136. The acetyl, propionyl and trifluoroacetyl derivatives of the primary and secondary regioisomeric amines were prepared and evaluated in GC–MS studies. The mass spectra for these derivatives were significantly individualized and the resulting unique fragment ions allowed for specific side chain identification. The trifluoroacetyl derivatives provided more fragment ions for molecular individualization among these regioisomeric substances. These trifluoroacetyl derivatives showed excellent resolution on a non-polar stationary phase such as Rtx-1.     

论WTO规则与中国外资法的修订

中国加入世界贸易组织,WTO规则对中国外资法提出了挑战,三部外资法的修改正是对这种挑战的回应。这种回应在很大程度上是及时且必要的,但也存在不足之处。同时,我国外资法体系中存在的大量的鼓励性措施,也应及时加强规范。     

Drug use and pretrial misconduct in New York city

Is recent drug use significantly associated with pretrial misconduct? Does consideration of recent drug use enhance risk classification among a sample of persons who have time free pending the disposition of their cases? Using data on arrestees in Manhattan, this paper examines these issues and some related questions. To measure recent drug use, urine samples were collected from persons shortly after their arrest and tested for four drugs: heroin, cocaine, PCP, and methadone. Two measures of pretrial misconduct are considered: whether a defendant fails to appear for a scheduled court date (FTA) and whether a defendant is rearrested prior to case disposition. Censored probit models are used to estimate the statistical association between drug test results and pretrial misconduct. Results show that drug test results are significantly associated with pretrial misconduct over and above the information typically available to judges at the time release decisions are made. Some implications of these findings for pretrial decision making are discussed.     

谈互联网上毒品犯罪及对策

互联网因其现代化、科学化、高效率、高覆盖及无国界等优点,迅速走进人们的生活,同时也给不法之徒进行犯罪活动提供了契机。目前通过互联网订购、销售毒品、进行在线毒品交易、提供制毒配方等互联网上涉毒案件已出现,网上毒品犯罪已成为全球毒品犯罪的最新发展动向,引起各国政府及执法部门的高度关注。     

反相高效液相色谱法同时测定人血清中氯丙嗪与氯扎平的浓度

作者建立了同时测定人血清中氯丙嗪与氯扎平浓度的反相高效液相色谱(RP—HPLC)法。以利眠宁为内标,用环己烷—乙醚(1:1)混合溶剂提取血清中氯丙嗪和氯扎平。高浓度中毒者血清(>1ug/ml)则用乙腈沉淀后直接进行色谱测定。血清中氯丙嗪、氯扎平的方法回收率乎均各为98.5±4.0(SD)%,CV4.2%;99.9±4.1(SD)%,CV4.1%。两药在血清中的最低检测浓度为10ng/ml。本法应用于临床氯丙嗪、氯扎平合用药物的血浓度监测及司法中毒案例的分析,结果满意。     

Relationships between concentrations of cocaine and its hydrolysates in peripheral blood, heart blood, vitreous humor and urine

Cocaine is known to degrade in vivo and in vitro by several hydrolytic mechanisms. A previous study found that the initial amount of cocaine added to plasma could be accounted for by summing the molar concentrations of cocaine's hydrolysis products and the cocaine remaining after hydrolysis. The present study was undertaken to investigate whether or not relationships might exist between such molar concentration sums for different postmortem bodily fluids. Determinations of cocaine, benzoylecgonine, ecgonine methyl ester, and ecgonine were performed using liquid chromatography/mass spectrometry (LC/MS/MS) with heart blood, femoral blood, vitreous humor (VH), and urine (UR). The results demonstrate a strong correlation between blood and VH concentrations (correlation coefficients of 0.88-0.94), weak correlation between the UR and blood concentrations (correlation coefficients of 0.61-0.64), and weak correlation between UR and VH concentrations (correlation coefficient of 0.59). The results demonstrate that ecgonine is a significant hydrolysate with concentrations on the same order of magnitude as benzoylecgonine. The results are consistent with rapid distribution of the parent drug and its hydrolysates in the blood and VH. The strong correlation between the blood and VH demonstrates that VH is an important medium for toxicology testing when attempting to make a determination of cocaine intoxication.