生物样品中杀鼠迷(Coumatetralyl)液相色谱检测的实验研究

本文经实验研究,确定了在酸性条件下用氯仿作为萃取剂,萃取液经酸性氧化铝柱净化,分段收集冼脱液的方法,使杀鼠迷回收率在不低于70%的基础上,脂肪及其它杂质近乎完全清除。杀鼠迷的检测采用高效液相色谱法(HPLC),紫外检测灵敏度达10~(-9)g。并对杀鼠迷中毒家兔的尿液进行了分析,发现一次性服用20mg/kg 体重的家兔,在45h 的尿液中,尚能检出其原形物。     

浅谈查缉战术模拟实战训练的组织与实施

模拟实战训练在查缉战术教学中起着至关重要的作用,在组织模拟实战训练时要制定好计划,在实施时,注意安全,还要积极开展教学总结。     

小鹅瘟病毒荧光抗体的制备与应用

将小鹅瘟病毒 (GPV )分离毒xw株接种健康兔获得兔抗GPV超免疫血清 ,由此制备兔抗GPV荧光抗体。采用直接荧光抗体试验对人工感染雏鹅、鹅胚及自然病例进行了检测。结果表明 ,该方法可检出患病组织中的病毒抗原 ,且肠和肝的含毒量高于尿囊细胞。     

网络攻击追踪方法的技术分析与实现模型

本文分析了IP地址追踪方法,结合现有的入侵检测技术提出了网络攻击源追踪系统的模型,阐述了该系统的体系结构和各部分的主要功能,给出了利用相关性分析对攻击者的攻击路径进行回溯的基本思想,对网络安全管理具有一定的借鉴意义。     

猪附红细胞体PPA-ELISA检测方法的建立

用物理方法纯化猪附红细胞体抗原,用纯化抗原免疫兔制备超免疫血清,以辣根过氧化物酶标记葡萄球菌A蛋白作为二抗,建立了辣根过氧化物酶标记葡萄球菌A蛋白的酶联免疫吸附试验(PPA ELISA)检测方法。结果显示,用PPA ELISA方法检测猪血液中的附红细胞体,具有很高的敏感性和特异性,重复性良好,完全适用于猪附红细胞体病的实验室诊断。     

移植时间和检测方法与allo-HSCT受者的DNA嵌合率及法医学意义

影响allo-HSCT受者DNA嵌合率的因素有很多:如受者的疾病类型、移植前预处理方案、移植时病情的严重程度、造血干细胞来源、局部的GVHD、HLA是否相合等。本文主要讨论与法医学关系最为密切的2个影响因素,即移植后时间和检测方法的灵敏度。法医物证学工作者在检案实践中,对allo-HSCT受者进行亲权鉴定或个体识别时,需注意移植后时间和检测方法的灵敏度对嵌合率的影响。女性受者接受男性供者HSCT的情况下,受者毛囊中可以检出供者Y染色体DNA,检案实践中来源于犯罪现场的生物检材不能仅仅检测Y染色体特异遗传标记,以免导致错误判断。     

Seeing is believing: observer perceptions of trait trustworthiness predict perceptions of honesty in high-stakes emotional appeals

Instantaneous first impressions of facial trustworthiness influence the manner in which observers evaluate ensuing information about stranger targets [e.g. Porter, S., &; ten Brinke, L. (2009). Dangerous decisions: A theoretical framework for understanding how judges assess credibility in the courtroom. Legal and Criminological Psychology, 14, 119–134. doi:10.1348/135532508X281520]. In two studies, we examined the association between perceptions of general trustworthiness and honesty assessments in an extremely high-stakes sample – individuals publicly pleading for the return of a missing relative, half of whom had killed the missing individual. In Study 1, observers (N?=?131) provided trustworthiness ratings – either before or after viewing and evaluating the honesty of videotaped or audio-only pleas – for a still image that depicted a neutral expression on the face of each pleader. In Study 2, observers (N?=?220) evaluated the sincerity of audio pleas paired either with an untrustworthy-looking target, a trustworthy-looking target, or no target face. Collectively, our findings indicated that first impressions of trait trustworthiness form the basis of state judgments of honesty, potentially contributing to misguided credibility assessments and miscarriages of justice in the legal system.     

Deciphering the riddle of human deceit detection: groups comprising a higher number of anxious people are better at distinguishing lies from truths

Are groups superior to individuals in detecting lies, and are there certain personality traits that significantly contribute to a collective lie-detecting capability? In the current research, we compared the ability of small groups to detect deception compared with individuals, and further examined whether small groups comprising more members high in attachment anxiety would show superior performance in detecting deceit. To this end, we asked 233 participants (40 groups and 113 individuals) to watch a series of clips showing a person making either truthful or untruthful statements, and then decide whether the speaker was honest or dishonest. Results confirmed our expectations and showed superior deceit-detection abilities in small groups, and that this ability was proportionate to the number of people high in attachment anxiety in the group. These results are discussed from the perspective of social defense theory, and the utility of diverse social groups in coping with diverse threats.     

两种包装方式对小体积生物检材DNA检验的影响

目的探讨一种适用于小体积生物物证检材的包装方式。方法选择M4型螺丝钉作为研究对象,经去污消毒处理,通过手握方式转移人体脱落细胞到其表面,对螺丝钉采用两种不同的包装方式(滤纸包裹和悬空固定),经KingFisher Flex自动提取工作站进行DNA提取,从基因座等位基因检出情况、STR分型谱带峰高、均衡性等方面比较两种包装方式对螺丝钉上DNA检出率的影响。结果悬空固定包装螺丝钉实验组获得的STR分型谱带峰高、均衡性等方面均优于滤纸包裹包装实验组,其在基因座检出数量、分型完全相同样本数及有效分型样本数方面也多于滤纸包裹包装实验组。结论为降低微量生物检材DNA二次转移造成的损耗,提高小体积生物检材DNA的检出率,建议对小体积生物检材采用悬空固定方式进行物证包装。     

Detection and visualization of human tears using alternate light sources for forensic purposes

The current scientific techniques for locating body fluids focus on quick and effective methodologies for easy and reliable identification. Efficient detection and identification of body fluids play a vital role in establishing the ‘corpus delecti’ of a crime. Non-destructive techniques such as the use of Alternate Light Sources (ALS) have been exploited for crime scene searches over large areas and detection of body fluids such as blood, semen, vaginal secretions, and saliva on a range of substrates. Tears are rarely found but can be considered as potential body fluid evidence due to their unique biochemical and molecular properties. Tears are secreted in response to physical or emotional stimuli. Due to the small volume of secretions, they are often overlooked in the crime scene. Tears may be found on surfaces such as clothing, bedding, tissue, handkerchief, or balaclava. The use of ALS to locate tears on tissue paper and fabric surfaces was tested which were not apparent to the naked eye. Tears stains were successfully detected on surfaces of forensic interest with varying sample ages up to three months with a broad excitation spectrum between 254 nm and 410 nm. Dried stains on tissue paper and fabric substrates were better detected with sharp margins, clear stain pattern visibility, and fluorescence intensity in comparison with moist and fresh stains. Tears stains can hence be detected with the use of ALS and suitable filter combinations under normal conditions and do not require any specific settings to locate them. These findings are suggestive for easy and quick identification of tears on large surfaces and as a presumptive test for forensic casework evidence examination.