离子色谱法检验生物检材中的氟乙酰胺（氟乙酸钠）

目的建立离子色谱法定性、定量分析氟乙酰胺(氟乙酸钠)的方法。方法用离子色谱检验生物检材中的氟乙酰胺。结果在生物检材中回收率>90%,得到了氟乙酸钠和氟离子的线性范围分别为500ng/g～20ng/g和1ng/g～1μg/g的线性回归方程,检出限分别为10ng/g和1ng/g,相对偏差均小于1%。结论该方法提取方法简单,可用于办案。     

Criterion Analysis and Content Validity for Standardized Behavioral Tests in a Detector-Dog Breeding Program

Many working-dog programs assess behavior during a dog's first year of life with the aim of predicting success in the field. However, decisions about which tests to administer are frequently made on the basis of tradition or intuition. This study reports results from a survey given to U.S.A.'s Transportation Security Administration (TSA) detection-dog handlers (N = 34). We categorized and summarized handlers’ responses regarding traits they felt were important for work. We used this criterion analysis to examine the content validity of the TSA's puppy tests. Results indicate that 13 of 15 traits that are currently being measured are relevant. However, several traits not currently measured were identified as being highly important, notably “play” and off-duty “calmness.” These results provide support that the TSA tests are measuring traits relevant to operational search team performance but also highlight other traits that may be profitable to assess in this and other detection-dog programs.     

折贪污贿赂案件的侦查

贪污犯罪历来为社会大众所不齿,其严重危害一直备受古今各国所关注。我国是社会主义国家,贪污犯罪更加为我国的基本制度所不容,因此,贪污犯罪的查办工作十分重要。本文从分析贪污案件查办的必要性入手,提出侦查贪污犯罪应当遵循依法侦查、实事求是、迅速及时、重证据不轻信口供等四个原则,然后归纳了贪污案件侦查中常用的方法,对贪污案件的侦查作了初步研究。     

论刑事侦查学基础理论唯理论特征

将侦查作为一种认识活动 ,并用哲学主客体论来描述侦查活动 ,长期以来这样的思维进路未经论证便成为侦查基础理论研究的方法。从语言逻辑角度看 ,通过抽象而寻求必然、追求绝对的理论具有唯理论特征。这一思维方式是特定历史情境下政治、哲学、文化等因素相互作用的结果 ,它充分体现了这些理论探求活动的意识形态特征。因此 ,改变理论研究中的思维方式 ,建立一种体现社会发展的开放性要求的对犯罪、侦查的新的知识表述 ,不仅具有理论意义 ,更对司法制度的改革具有重要而又现实的实践价值。     

论信息化背景下的侦查工作

随着计算机技术和现代通信技术的发展，信息网络延伸至社会生活的各个角落，作为社会副产品的犯罪也随之演变出多种新的犯罪表现，使传统侦查工作在证据获取和破案模式上面临挑战。基于此，信息化背景下的侦查工作，可从侦查模式、工作方法、侦查观念和提高侦查力水平上寻求突破，以期顺应时代要求，更好地履行职责。     

街头诈骗案件的研究与对策

街头诈骗案件社会危害性较大，直接侵害人民群众的切身利益。由于案件的特殊性，侦破难度较大。现有的公安刑侦资料，又鲜有关于打击街头诈骗这一类案件的研究文献。笔者结合自身工作经验，用实证的研究方式，对街头诈骗案件的表现形式、危害性、特点、侦破对策、防范措施等问题进行一番较为系统的探讨。     

鸡肿瘤病快速鉴别诊断试剂盒的研制及其应用

建立了快速鉴别诊断鸡肿瘤病的PCR技术 ,研制鸡肿瘤病快速鉴别诊断试剂盒。应用该试剂盒在两年多时间里 ,对来源于广西养鸡业发达地区的 13 9个鸡群共 5 0 5只病、死鸡的 15 70份肿瘤及可疑肿瘤组织病料进行了检测 ,马立克氏病、网状内皮增生症和禽白血病的阳性率分别为 74.0 6%、11.49%和 6.73 % ,肿瘤病的混合发生率为 16.83 %。结果表明 ,应用PCR技术建立的鸡肿瘤病快速鉴别诊断试剂盒具有操作简便快捷、结果准确可靠、保存方便等特点 ,不仅适用于临床肿瘤病的鉴别诊断 ,而且对开展肿瘤病的流行病学调查及兽医检疫具有重要意义     

浅论对侦查行为的司法审查制度

对侦查行为由法院统一进行司法审查 ,是现代民主国家的普遍做法。我国之所以未能建立这种司法审查制度 ,主要原因在于“侦查中心主义”的诉讼构造和检察机关法律监督职能的影响以及法院的地位缺乏权威等。出于实现惩罚犯罪与保障人权的诉讼目的、贯彻决定与执行分离的原则、加强司法救济以及与国际刑事法治接轨的需要 ,我国也应当建立对侦查行为的司法审查制度 ,具体内容包括如实行司法令状制度和非法证据排除规则 ,明确规定侦查行为的可诉性等     

Screening Biological Stains with qPCR versus Lateral Flow Immunochromatographic Test Strips: A Quantitative Comparison using Analytical Figures of Merit

Biological fluid identification is an important facet of evidence examination in forensic laboratories worldwide. While identifying bodily fluids may provide insight into which downstream DNA methods to employ, these screening techniques consume a vital portion of the available evidence, are usually qualitative, and rely on visual interpretation. In contrast, qPCR yields information regarding the amount and proportion of amplifiable genetic material. In this study, dilution series of either semen or male saliva were prepared in either buffer or female blood. The samples were subjected to both lateral flow immunochromatographic test strips and qPCR analysis. Analytical figures of merit—including sensitivity, minimum distinguishable signal (MDS) and limit of detection (LOD)—were calculated and compared between methods. By applying the theory of the propagation of random errors, LODs were determined to be 0.05 μL of saliva for the RSID? Saliva cards, 0.03 μL of saliva for Quantifiler^® Duo, and 0.001 μL of semen for Quantifiler^® Duo. In conclusion, quantitative PCR was deemed a viable and effective screening method for subsequent DNA profiling due to its stability in different matrices, sensitivity, and low limits of detection.