丝状霉形体山羊亚种血清抗体间接ELISA检测方法的建立

利用丝状霉形体山羊亚种标准株PG3制备抗原,经纯化后作为包被抗原建立了间接ELISA方法,用于检测丝状霉形体山羊亚种血清抗体。经方阵滴定确定最佳抗原包被浓度为1.09μg/mL,血清样品最佳稀释度为1∶64,兔抗羊IgG辣根过氧化物酶结合物的最佳稀释度为1∶40 000,抗原抗体最佳结合时间为1 h。判定标准为样品D490 nm值与标准阴性血清D490 nm值之比(P/N)≥3为阳性,≤2.5为阴性,介于二者之间的为可疑。重复性和稳定性试验证明,建立的间接ELISA的稳定性和重复性良好,与间接血凝试验相比,其敏感性较高。     

蛋鸡血清与卵黄中禽流感病毒抗体的相关性

用禽流感 (AI)H9N2油乳剂灭活疫苗免疫非免疫蛋鸡 ,并于免疫后第 0、3、5、7、9、11、13、15、17、19、2 1、30d分别测定血清及卵黄中的AI血凝抑制试验 (HI)及琼脂扩散凝集试验(AGP)抗体。结果显示 ,免疫后第 7d卵黄中即可检出HI及AGP抗体 ,以后逐渐上升 ,在免疫后第 19～ 2 1d达高峰值 ,与血清的HI抗体水平接近 ,差异不显著 (P >0 .0 5 )。表明通过对鸡蛋卵黄AIV抗体检测 ,可以进行AI的疫情监测 ,但卵黄抗体检测具有一定的滞后性     

抗α-1酸性糖蛋白血清的制备

作者用分离纯化的α-1酸性糖蛋白免疫新西兰兔,制备抗α-1酸性糖蛋白血清。鉴定结果表明,制备的抗血清与进口商品抗α-1酸性糖蛋白血清特异性相同;与人血清其他蛋白无交叉反应。琼脂双向扩散法测得抗血清效价为128,检出α-1酸性糖蛋白的最低浓度为204μg/ml。     

Ultraviolet 365 as an Alternative Light Source for Detection of Blood Serum

The use of alternative light sources (ALS) in bloodstain analysis has focused on dried (whole) blood, while information on detection of blood serum is lacking. Serum detection by ALS could provide valuable information at a crime scene, as serum may become separated from blood during clotting and cast off, especially in cases where the victim is moved. Additionally, a perpetrator may concentrate on the removal/scouring of dried blood with small amounts of serum going unnoticed, as it dries relatively clear on certain objects. In this report, the detection of human blood serum was evaluated using ultraviolet (UV) light at two different wavelengths. These results show that ultraviolet (UV) at 365 nm (UV365) was effective in the detection of even small amounts of blood plasma and serum, compared with UV at 395 nm, which was not. UV365 was also found to be useful in distinguishing blood imprints from clotting blood which had been transferred to material versus blood that had been added directly. Taken together, these results demonstrate that UV365 may be utilized as a simple, nondestructive method for blood serum detection.     

The Estimation of Postmortem Serum Urea via the Ultrafiltration of Hemolyzed Blood

Postmortem serum urea has been demonstrated as an objective indicator for the forensic diagnosis of cause of death. However, samples used in postmortem biochemical analysis are always affected by hemolysis. To investigate whether hemolysis affects the biochemical analysis of urea and to explore the feasibility of using ultrafiltration to process hemolyzed blood samples, three different levels of hemolyzed blood samples were used to assess the influence of hemolysis on postmortem biochemical analysis of urea, and two ultrafiltration methods were used to process the hemolyzed blood samples. Bias% was used to assess the interference of hemolysis. Our results showed that heavy hemolysis had a significant influence on the biochemical analysis of urea. Both ultrafiltration methods in the present study could significantly reduce the interference of hemolysis, with the |bias%| of methods A and B decreasing from 69.74% ± 99.14% to 12.18% ± 7.23% and 10.77% ± 8.09%, respectively, compared to the original serum. After regression correction, there was no significant difference between the urea concentration in the ultrafiltrates of the two ultrafiltration methods and that in the original serum, which suggested that the postmortem serum urea concentration could be estimated by the corrected urea concentration in the ultrafiltrate. The current study also provided possible pretreatment methods for postmortem biochemical analysis of other biomarkers in hemolyzed blood samples of forensic practice.     

野生鸡枞菌多糖的提取及其免疫活性分析

采用水提醇沉法获得鸡枞菌多糖,并对其总糖含量进行了测定;研究了不同剂量的鸡枞菌多糖对正常小鼠的免疫器官指数、血清溶血素水平、腹腔巨噬细胞吞噬功能及脾淋巴细胞增殖等免疫指标的影响。结果:鸡枞菌多糖得率为6.27%,总糖含量为94%;根据体重腹腔注射20mg/kg的鸡枞菌多糖可明显提高正常小鼠的溶血素水平(P<0.01)、脾指数(P<0.01)、胸腺指数(P<0.05)和巨噬细胞吞噬功能(P<0.01);体外试验中,与空白对照组相比,25μg/mL的鸡枞菌多糖可促进体外脾淋巴细胞增殖(P<0.05),12.5μg/mL鸡枞菌多糖对ConA或LPS引起的脾淋巴细胞增殖均有显著的协同作用(P<0.01)。结果表明,鸡枞菌多糖能显著增强小鼠的体液免疫和细胞免疫功能,有望成为一种新的天然免疫增强剂。     

中西医结合治疗重症蝮蛇咬伤26例

目的 回顾重症蝮蛇咬伤患者的病历资料,总结重症蝮蛇咬伤的治疗经验。 方法 对26例重症蝮蛇咬伤患者采用中西医结合疗法进行治疗。西医疗法以局部扩创排毒、注射抗蝮蛇毒血清及对症治疗为主,对危重症患者应用机械通气、血液净化、重症监护等治疗技术;中医疗法以内服院内协定方蛇伤冲剂及采用季德胜蛇药片内服外敷为主。结果 治愈22例,好转3例,无效1例,总有效率为96.1%。发生脏器功能损害26例,治疗后恢复25例。住院时间2～16 d,平均(7.72±1.35)d。结论 中西医结合治疗重症蝮蛇咬伤,治愈率高,脏器功能损害恢复好,住院时间短。     

电针大脑中动脉闭塞模型大鼠血清减轻体外新生乳鼠神经细胞的缺糖低氧损伤的作用研究

目的 观察"电针血清"对新生乳鼠皮质神经细胞糖氧剥夺/复氧(oxygen-glucose depriva-tion and reoxygenation,OGD/R)损伤的作用.方法 将成年SD大鼠随机分为对照组、模型组、电针组,采用电凝法复制大脑中动脉闭塞模型;7d后对各组大鼠进行腹主动脉取血,离心,抽取上清,分离新生...     

樗白皮提取物对小白鼠体内黄嘌呤氧化酶活性的影响

为了研究樗白皮提取物对小白鼠机体内黄嘌呤氧化酶(XOD)活性的影响,将实验动物分为5、10、30 g/kg樗白皮提取物灌喂组和正常对照组,对各组动物血清、脾、肝中XOD活性进行了测定。结果,10 g/kg樗白皮提取物能够降低小白鼠血清、脾、肝组织中的XOD活性,30 g/kg樗白皮提取物能提高上述各组织中的XOD活性,5 g/kg樗白皮提取物组小白鼠组织中的XOD活性接近正常对照组。结果表明,不同剂量的樗白皮提取物对小白鼠体内XOD活性的作用不同,低剂量时具有抗氧化作用,高剂量时表现为促氧化作用。     

人结合珠蛋白抗血清的制备

本文介绍了离子交换色谱及凝胶过滤色谱,从人血浆中初步纯化出人结合珠蛋白(HAPTOGLOBIN,HP)抗原,对家兔进行免疫制备出Hp粗抗血清,再经固相吸收精制获得特异性的抗Hp血清。