测定血痕MN型的血清制备

用免疫吸收方法制备的抗-M与抗-N血清,检测10天内的不相应的MN血型血痕和大约2年的相应MN型血痕,凡能获得特异性结果的抗血清才能用于血痕的MN血痕测定。否则,只能用于测定血液的MN血型。     

反相高效液相色谱法同时测定人血清中氯丙嗪与氯扎平的浓度

作者建立了同时测定人血清中氯丙嗪与氯扎平浓度的反相高效液相色谱(RP—HPLC)法。以利眠宁为内标,用环己烷—乙醚(1:1)混合溶剂提取血清中氯丙嗪和氯扎平。高浓度中毒者血清(>1ug/ml)则用乙腈沉淀后直接进行色谱测定。血清中氯丙嗪、氯扎平的方法回收率乎均各为98.5±4.0(SD)%,CV4.2%;99.9±4.1(SD)%,CV4.1%。两药在血清中的最低检测浓度为10ng/ml。本法应用于临床氯丙嗪、氯扎平合用药物的血浓度监测及司法中毒案例的分析,结果满意。     

降解生物检材遗传标记检测的研究进展

降解生物检材遗传标记的检测一直是法医学实践和科研的难点。虽然PCR-STR分析技术能在一定程度上检测降解生物检材的遗传标记,但是对于某些高度降解的生物检材如骨骼、牙齿等的PCR-STR分型仍旧十分困难,存在不稳定性和分型异常现象。本文就近几年来PCR-STR分型技术在降解生物检材的法医实践中出现的问题和解决方法作一综述。     

吸食新型毒品的特点及其防控对策

新型毒品被专家称为“21世纪毒品”,在我国蔓延迅速,大有取代传统毒品海洛因成为吸毒者最主要消费品之势,已构成对我国禁毒的新威胁,并已引起党和政府高度重视,必须采取有效的防范和控制手段,减少吸食新型毒品的人数,将禁毒人民战争进一步推向深入。     

Genetic polymorphism at 15 tetrameric short tandem repeat loci in four aboriginal tribal populations of Bengal

POPULATIONS: This study reports the genetic polymorphism observed at 15 short tandem repeat loci D3S1358, TH01, D21S11, D18S51, D5S818, D13S317, D7S820, D16S539, CSF1PO, vWA, D8S1179, TPOX, D2S1338, D19S433, and FGA in four aboriginal populations of Bengal. The analysis was performed to decipher the suitability of CODIS as well as six other highly polymorphic and unlinked markers in Forensic Testing. Studied populations include four tribes: Karmali, Kora, Maheli, and Lodha.     

Analysis of Y-STR loci in a population sample from northeast China

POPULATION: A total of 141 unrelated Chinese Han male individuals living in Liaoning in northeast China.     

Y chromosome STR haplotypes and allele frequencies in Illinois Caucasian, African American, and Hispanic males

POPULATION: Population: Illinois Caucasian ( n =117), Illinois African American ( n =218), and Illinois Hispanic ( n =68).     

Alpha-amylase kinetic test in bodily single and mixed stains

Recently, in Italy, a murder and a putative sexual violence was accomplished on a child. A bodily fluids mixture on the child's underwear between the victim (female) and the suspect (male) was ascertained by short tandem repeat (STR) DNA typing and, due to the absence of seminal fluid, saliva from the suspect and urine from the child was hypothesized. In order to investigate the possibility of specifically and rapidly detecting saliva stains both alone and mixed with other bodily fluids, we used a quantitative spectrophotometric technique, named Amylase test, for the detection of alpha-amylases. We determined alpha-amylase activity and reaction kinetic curves in several samples collected from the child's underwear. In order to confirm our intuition, we first tested saliva, perspiration, and urine, singularly and in mixtures; second, several forensic stains including saliva, perspiration, urine stains, saliva/perspiration, and saliva/urine mixture stains were tested. Evaluating alpha-amylase activity values and time-course curves' behavior of alpha-amylase reactions we were able to recognize successfully, in all cases, the presence of saliva and to distinguish it specifically from other bodily fluids containing alpha-amylase. A further confirmation of our result was provided by STR DNA typing on several areas of the underwear: a clear correlation between alpha-amylases activity and male DNA was detected on all the samples evaluated.